Cell No. : Cell Name RCB1731 : TM-31  update : 2023/01/06
Comment			Human cell line derived from astrocytoma developed in a patient with NF-1 (neurofibromatosis type 1)
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kurimoto, Masanori
		Originator	Kurimoto, Masanori
		Year of deposit	2001
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	42 years
		Tissue	brain
		Disease name	malignant astrocytoma
		Classification	cancer
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_6735
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		72-153(50) : /74(8),75(7),76(7),77(8),78(1),142(1)/
		STR(human)		OK
Reference information		Reference	1
		User's Publication	2

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Reference

2694  Kurimoto M, Hirashima Y, Ogiichi T, Hamada H, Kamiyama H, Endo S  Establishment and characterization of a novel malignant astrocytoma cell line derived from a tumor removed in a patient with neurofibromatosis type 1.  J Neurosurg  2001  94:301-8  PubMed ID: 11213969   DOI: 10.3171/jns.2001.94.2.0301

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User's Publication

19506  Dougherty J, Harvey K, Liou A, Labella K, Moran D, Brosius S, De Raedt T.  Identification of therapeutic sensitivities in a spheroid drug combination screen of Neurofibromatosis Type I associated High Grade Gliomas  PLoS One  2023  18(2):e0277305  PubMed ID: 36730269   DOI: 10.1371/journal.pone.0277305

4672  Ohka F, Shinjo K, Deguchi S, Matsui Y, Okuno Y, Katsushima K, Suzuki M, Kato A, Ogiso N, Yamamichi A, Aoki K, Suzuki H, Sato S, Arul Rayan N, Prabhakar S, Göke J, Shimamura T, Maruyama R, Takahashi S, Suzumura A, Kimura H, Wakabayashi T, Zong H, Natsume A, Kondo Y.  Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-wild-type Diffuse Astrocytic Gliomas.  Cancer Res  2019  79(19):4814-4827  PubMed ID: 31431463   DOI: 10.1158/0008-5472.CAN-19-1272