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RCB1709 : GE1  update : 2018/08/01
CommentMouse-derived gingival epithelial cell line. Derived from SV40-Large T antigen transgenic mouse. Application consideration
Comment from the depositor
Restriction (Category)f
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.2811(J Oral Pathol Med 2001;30:296-304) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Hatakeyama, Setsuko
Originator Hatakeyama, Setsuko
Year of deposit 2000
Animal mouse < Mammals
Strain name C57BL/6
Gender Female
Age at sampling 2.5 months
Tissue gingival kerarinocyte
Classification transformed
Recombinant recombinant
Exogene pSVtsA58
Year of origin 1996
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives SFM-101 + 1% FBS + 10ng/ml mouse EGF
Antibiotics Free
Passage method (0.24% trypsin + 0.08% EDTA) or (0.25% trypsin + 0.02% EDTA), washed twice with medium
Culture information Passage cell No 4 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 8

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2811  Hatakeyama S, Ohara-Nemoto Y, Yanai N, Obinata M, Hayashi S, Satoh M  Establishment of gingival epithelial cell lines from transgenic mice harboring temperature sensitive simian virus 40 large T-antigen gene.  J Oral Pathol Med  2001  30:296-304 

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User's Publication
7446  Kuramitsu-Fujimoto S, Ariyoshi W, Saito N, Okinaga T, Kamo M, Ishisaki A, Takata T, Yamaguchi K, Nishihara T.  Novel biological activity of ameloblastin in enamel matrix derivative.  J Appl Oral Sci  2015  23:49-55  PubMed ID: 25760267
8104  Hatakeyama S, Mizusawa N, Tsutsumi R, Yoshimoto K, Mizuki H, Yasumoto S, Sato S, Takeda Y.  Establishment of human dental epithelial cell lines expressing ameloblastin and enamelin by transfection of hTERT and cdk4 cDNAs.  J. Oral Pathol. Med.  2010  40:227-34  PubMed ID: 20923449
8401  Akihiro Toshinaga, Ryuji Hosokawa, Toshinori Okinaga, Chihiro Masaki, Toshiyuki Tsujisawa, Tatsuji Nishihara  Inflammatory response in epithelial cells induced by mechanical stress is suppressed by hyaluronic acid  Inflammation and Regeneration  2010  30:120-127 
9628  Iwanabe Y, Masaki C, Tamura A, Tsuka S, Mukaibo T, Kondo Y, Hosokawa R.  The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells.  J Prosthodont Res  2016  60:308-314  PubMed ID: 27026212
9777  Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Matsumura H, Izawa T, Tanaka E, Ganss B, Ogata Y.  Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFβ1 in mouse gingival epithelial cells.  Apoptosis  2016  21:1057-70  PubMed ID: 27502207
10818  Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Ganss B, Ogata Y.  TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells.  J Oral Sci  2018    PubMed ID: 29657250
11191  Noda K, Yamazaki M, Iwai Y, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y.  IL-1β and TNF-α regulate mouse amelotin gene transcription in gingival epithelial cells.  J Oral Sci  2018    PubMed ID: 30158339
11321  Nakayama Y, Tsuruya Y, Noda K, Yamazaki-Takai M, Iwai Y, Ganss B, Ogata Y.  Negative feedback by SNAI2 regulates TGFβ1-induced amelotin gene transcription in epithelial-mesenchymal transition.  J. Cell. Physiol.  2019    PubMed ID: 30488439

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