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RCB1695 : RMS-YM
update : 2020/01/14
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| Comment | Embryonal rhabdomyosarcoma cell line derived from human. Transplantable in nude mouse. |
| Comment from the depositor | |
| Terms and conditions | Basically, there is no restriction regarding academic use. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Naoe, Tomoki
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| Originator |
Kubo, Kazuaki
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| Year of deposit |
2000
|
| Animal |
human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Male
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| Age at sampling |
2 years
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| Tissue |
urachus
|
| Case history |
embryonal rhabdomyosarcoma
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| Classification |
cancer
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| Year of origin |
1988
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|
Lifespan |
infinite
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| Morphology |
fibroblast-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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RPMI1640 + 20mM HEPES + 10% FBS (heat inactivated) + 0.1mM NEAA
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| Antibiotics |
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Free
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|
Passage method |
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0.25% trypsin + 0.02% EDTA
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| STR (human) |
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OK
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| Isozyme |
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LD, NP
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| Chromosome mode |
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52-55(50) : 52(2),53(18),54(26),55(4)
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| Reference information |
Reference |
1
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| User's Publication |
2
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| User's Publication |
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9540
Zenitani M, Nojiri T, Uehara S, Miura K, Hosoda H, Kimura T, Nakahata K, Miyazato M, Okuyama H, Kangawa K.
C-type natriuretic peptide in combination with sildenafil attenuates proliferation of rhabdomyosarcoma cells.
Cancer Med
2016
5:795-805
PubMed ID: 26816265
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7155
Kuda M, Kohashi K, Yamada Y, Maekawa A, Kinoshita Y, Nakatsura T, Iwamoto Y, Taguchi T, Oda Y.
FOXM1 expression in rhabdomyosarcoma: a novel prognostic factor and therapeutic target.
Tumour Biol.
2015
37:5213-23
PubMed ID: 26553361
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