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RCB1695 : RMS-YM  update : 2020/01/14
CommentEmbryonal rhabdomyosarcoma cell line derived from human. Transplantable in nude mouse.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Naoe, Tomoki
Originator Kubo, Kazuaki
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 2 years
Tissue urachus
Case history embryonal rhabdomyosarcoma
Classification cancer
Year of origin 1988
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 20mM HEPES + 10% FBS (heat inactivated) + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Chromosome mode 52-55(50) : 52(2),53(18),54(26),55(4)
Reference information Reference 1
User's Publication 2

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2833  Kubo K, Naoe T, Utsumi KR, Ishiguro Y, Ueda K, Shiku H, Yamada K  Cytogenetic and cellular characteristics of a human embryonal rhabdomyosarcoma cell line, RMS-YM.  Br J Cancer  1991  63:879-84  PubMed ID: 1906291

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User's Publication
9540  Zenitani M, Nojiri T, Uehara S, Miura K, Hosoda H, Kimura T, Nakahata K, Miyazato M, Okuyama H, Kangawa K.  C-type natriuretic peptide in combination with sildenafil attenuates proliferation of rhabdomyosarcoma cells.  Cancer Med  2016  5:795-805  PubMed ID: 26816265
7155  Kuda M, Kohashi K, Yamada Y, Maekawa A, Kinoshita Y, Nakatsura T, Iwamoto Y, Taguchi T, Oda Y.  FOXM1 expression in rhabdomyosarcoma: a novel prognostic factor and therapeutic target.  Tumour Biol.  2015  37:5213-23  PubMed ID: 26553361

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