Cell No. : Cell Name RCB1692 : Tpit/E  update : 2023/01/06
Comment			Vascular endothelial cell-like cell line derived from an anterior pituitary gland of a transgenic mouse expressing temperature sensitive SV40 large T antigen. Producing endothelin.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Inoue, Kinji
		Originator	Inoue, Kinji
		Year of deposit	2000
		Animal	_mouse < Mammals
		Strain name	C57BL
		Gender	Unknown
		Age at sampling	1 month
		Tissue	anterior pituitary gland
		Classification	semi-normal
		Recombinant	recombinant
		Exogene	large T
		Year of origin	1995
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_U899
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
		Antibiotics		Free
		Passage method		(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
	Culture information	Passage ratio		1 : 4-8 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		33 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Reference information		Reference	2
		User's Publication	1

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Reference

6664  Saishu Yoshida, Masashi Higuchi, Hiroki Ueharu, Naoto Nishimura, Mitsuyoshi Tsuda, Hideji Yako, Mo Chen, Hideo Mitsuishi, Yoshiya Sano, Takako Kato, Yukio Kato  Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF  J Reprod Dev  2014  60(4):295-303  PubMed ID: 24881870   DOI: 10.1262/jrd.2014-031

2683  Chen L, Maruyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y, Inoue K.  Cytological characterization of a pituitary folliculo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion  Endocrinology  2000  141:3603-3610  PubMed ID: 11014214   DOI: 10.1210/endo.141.10.7710

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User's Publication

17938  Yoshiura K, Nishishita T, Nakaoka T, Yamashita N, Yamashita N.  Inhibition of B16 melanoma growth and metastasis in C57BL mice by vaccination with a syngeneic endothelial cell line  J Exp Clin Cancer Res  2009  28(1):13  PubMed ID: 19183492   DOI: 10.1186/1756-9966-28-13