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RCB1681 : GS-HepG2  update : 2018/08/01
CommentA subline of HepG2. Expressing abundant glutamine synthetase. Information
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Omasa, Takeshi
Originator Omasa, T. & Suga, K.
Year of deposit 2000
Original cell Hep G2
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue liver
Case history hepatocyte carcinoma
Classification cancer
Recombinant recombinant
Exogene pBK-CMV-GS
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RDF (Glutamine(-)) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 46-52(50) : 46(2),47(2),48(6),49(10),50(16),51(13)/
Images
deposit info
lot info
Reference information Reference 6
User's Publication 2


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Reference
2830    Enisawa, S. et al  Jpn J Artif  1996  25:399-402 
465  Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.  Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B  Nature  1979  282:615-6  PubMed ID: 233137
1155  Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.  Knowles, B B, Howe, C C, Aden, D P  Science  1980  209:497-9  PubMed ID: 6248960
4984  Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.  Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.  Carcinogenesis  1980  1(10):871-5  PubMed ID: 11219860
2831  An attempt to add biological functions by genetic engineering in order to produce high-performance bioreactor cells for hybrid artificial liver: transfection of glutamine synthetase into Chinese hamster ovary (CHO) cell.  Enosawa S, Suzuki S, Fujino M, Amemiya H, Omasa T, Urayama S, Tanimura N, Suga K.  Cell Transplant  1997  6(5):537-40  PubMed ID: 9331509
2832  Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system  Takeshi Omasa et al  Enzyme and Microbial Technology  2004  35:519-524 

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User's Publication
2076  Examination of 7-ethoxycoumarin deethylation and ammonia removal activities in 31 hepatocyte cell lines.  Enosawa S, Suzuki S, Kakefuda T, Amemiya H  Cell Transplant  1996  5:S39-40  PubMed ID: 8889228
9019  Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system.  Omasa T, Kim K, Hiramatsu S, Katakura Y, Kishimoto M, Enosawa S, Ohtake H.  Biotechnol. Prog.  2005  21:161-7  PubMed ID: 15903254



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