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RCB1648 : Hep G2  update : 2019/12/16
CommentHepatocellular carcinoma. Produce alpha-fetoprotein, albumin, etc. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011430 prior to order. Information
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011430 prior to order.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 15 years
Tissue liver
Case history hepatocyte carcinoma
Classification cancer
History overseas, Dr.Knowles (Wistar Institute, Ohiladelphoa) -> ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 51-53(50) : 51(12).52(22),53(16)
Reference information Reference 3
User's Publication 31

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1155  Knowles, B B, Howe, C C, Aden, D P  Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.  Science  1980  209:497-9  PubMed ID: 6248960
4984  Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.  Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.  Carcinogenesis  1980  1(10):871-5  PubMed ID: 11219860
465  Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B  Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.  Nature  1979  282:615-6  PubMed ID: 233137

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User's Publication
11540  Ogura S, Tameda M, Sugimoto K, Ikejiri M, Usui M, Ito M, Takei Y.  A substitution in the pre-S1 promoter region is associated with the viral regulation of hepatitis B virus.  Virol. J.  2019    PubMed ID: 31046787
4214  Matsumura K, Imai H, Go Y, Kusuhara M, Yamaguchi K, Shirai T, Ohshima K.  Transcriptional activation of a chimeric retrogene PIPSL in a hominoid ancestor.  Gene  2018    PubMed ID: 30096459
4216  Feng R, Morine Y, Ikemoto T, Imura S, Iwahashi S, Saito Y, Shimada M.  Nrf2 activation drive macrophages polarization and cancer cell epithelial-mesenchymal transition during interaction.  Cell Commun. Signal  2018    PubMed ID: 30180849
9973  Kawakami K, Moritani C, Uraji M, Fujita A, Kawakami K, Hatanaka T, Suzaki E, Tsuboi S.  Hepatoprotective effects of rice-derived peptides against acetaminophen-induced damage in mice.  J Clin Biochem Nutr  2017  60:115-120  PubMed ID: 28366990
10185  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773
10595  Uemura T, Takasaka T, Igarashi K, Ikegaya H.  Spermine oxidase promotes bile canalicular lumen formation through acrolein production.  Sci Rep  2017  7:14841  PubMed ID: 29093526
10020  Moriyama K, Naito S, Wakabayashi R, Goto M, Kamiya N.  Enzymatically prepared redox-responsive hydrogels as potent matrices for hepatocellular carcinoma cell spheroid formation.  Biotechnol J  2016  11:1452-1460  PubMed ID: 27617786
4039  Oshikata-Miyazaki A, Takezawa T.  Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber.  Cytotechnology  2016  68(5):1801-11  PubMed ID: 26660096
7456  Okina Y, Takeuchi F, Yokomichi T, Takada Y, Kataoka T.  Cardenolide aglycones inhibit tumor necrosis factor α-induced expression of intercellular adhesion molecule-1 at the translation step by blocking Na?/K?-ATPase.  Biol. Pharm. Bull.  2015  38:39-47  PubMed ID: 25744456
7501  Lai YT, Sato M, Ohta S, Akamatsu K, Nakao S, Sakai Y, Ito T.  Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique.  Colloids Surf B Biointerfaces  2015  127:1-7  PubMed ID: 25635614
7574  Yuji SHIBATA, Katsuhisa YAMADA, Chiaki IMADA, Takeshi KOBAYASHI, Takeshi TERAHARA  Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan.  Deep Ocean Water Research  2015  15:117-124 
5372  Nakajima Y, Kanno T, Nagaya T, Kuribayashi K, Nakano T, Gotoh A, Nishizaki T.  Adenosine deaminase inhibitor EHNA exhibits a potent anticancer effect against malignant pleural mesothelioma.  Cell Physiol Biochem  2015  35(1):51-60  PubMed ID: 25547995
5844  Kanno T, Tanaka A, Shimizu T, Nakano T, Nishizaki T.  1-[2-(2-Methoxyphenylamino)ethylamino]-3-(naphthalene-1-yloxy)propan-2-ol as a potential anticancer drug.  Pharmacology  2013  91(5-6):339-45  PubMed ID: 23817168
7642  Tagaya M, Yamazaki T, Tsuya D, Sugimoto Y, Hanagata N, Ikoma T.  Nano/microstructural effect of hydroxyapatite nanocrystals on hepatocyte cell aggregation and adhesion.  Macromol Biosci  2011  11:1586-93  PubMed ID: 22052565
7683  Fujii T, Minagawa T, Shimizu T, Takeguchi N, Sakai H.  Inhibition of ecto-ATPase activity by curcumin in hepatocellular carcinoma HepG2 cells.  J Physiol Sci  2011  62:53-8  PubMed ID: 21932081
7988  Yasuda A, Natsume M, Osakabe N, Kawahata K, Koga J.  Cacao polyphenols influence the regulation of apolipoprotein in HepG2 and Caco2 cells.  J. Agric. Food Chem.  2011  59:1470-6  PubMed ID: 21226458
8211  Okuyama T, Yamazoe H, Mochizuki N, Khademhosseini A, Suzuki H, Fukuda J.  Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device.  J. Biosci. Bioeng.  2010  110:572-6  PubMed ID: 20591731
8234  Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J.  Cell micropatterning inside a microchannel and assays under a stable concentration gradient.  J. Biosci. Bioeng.  2010  110:230-7  PubMed ID: 20547384
8237  Sakai Y, Yoshida S, Yoshiura Y, Mori R, Tamura T, Yahiro K, Mori H, Kanemura Y, Yamasaki M, Nakazawa K.  Effect of microwell chip structure on cell microsphere production of various animal cells.  J. Biosci. Bioeng.  2010  110:223-9  PubMed ID: 20547385
8705  Hattori N, Suzuki T, Jinno S, Okeya H, Ishikawa A, Kondo C, Hayashi T, Ito M, Kanamori T, Kawai T, Noguchi T.  Methyl methacrylate activates the Gsta1 promoter.  J. Dent. Res.  2008  87:1117-21  PubMed ID: 19029078
8720  Mori R, Sakai Y, Nakazawa K.  Micropatterned organoid culture of rat hepatocytes and HepG2 cells.  J. Biosci. Bioeng.  2008  106:237-42  PubMed ID: 18929998
8765  Saigo K, Yoshida K, Ikeda R, Sakamoto Y, Murakami Y, Urashima T, Asano T, Kenmochi T, Inoue I.  Integration of hepatitis B virus DNA into the myeloid/lymphoid or mixed-lineage leukemia (MLL4) gene and rearrangements of MLL4 in human hepatocellular carcinoma.  Hum. Mutat.  2008  29:703-8  PubMed ID: 18320596
8786  Tamura T, Sakai Y, Nakazawa K.  Two-dimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip.  J Mater Sci Mater Med  2007  19:2071-7  PubMed ID: 17968500
3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080
9172  Harada K, Ohba K, Ozaki S, Isse K, Hirayama T, Wada A, Nakanuma Y.  Peptide antibiotic human beta-defensin-1 and -2 contribute to antimicrobial defense of the intrahepatic biliary tree.  Hepatology  2004  40:925-32  PubMed ID: 15382127
3793  Yoshida S, Honda A, Matsuzaki Y, Fukushima S, Tanaka N, Takagiwa A, Fujimoto Y, Miyazaki H, Salen G.  Anti-proliferative action of endogenous dehydroepiandrosterone metabolites on human cancer cell lines.  Steroids  2003  68(1):73-83  PubMed ID: 12475725
3794  Sakai Y, Fukuda O, Choi SH, Sakoda A.  Development of a biohybrid simulator for absorption and biotransformation processes in humans based on in vitro models of small intestine and liver tissues.  J Artif Organs  2003  6(4):273-81  PubMed ID: 14691670
9400  Ikeda T, Zhang J, Chano T, Mabuchi A, Fukuda A, Kawaguchi H, Nakamura K, Ikegawa S.  Identification and characterization of the human long form of Sox5 (L-SOX5) gene.  Gene  2002  298:59-68  PubMed ID: 12406576
3787  Mikiko Shimizu, Hiro-omi Tamura  Identification and Localization of Two Hydroxysteroid Sulfotransferases in the Human Brain  Journal of Health Science  2002  48:467-472 
3773  Hirano R, Igarashi O, Kondo K, Itakura H, Matsumoto A.  Regulation by long-chain fatty acids of the expression of cholesteryl ester transfer protein in HepG2 cells.  Lipids  2001  36(4):401-6  PubMed ID: 11383693
6400  Hatano H, Kawashima H, Ogose A, Hotta T, Endo N.  A PCR-ELISA assay for the detection of disseminated osteosarcoma cells in a mouse metastatic model.  J Orthop Sci  2001  6(3):269-75  PubMed ID: 11484122

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