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RCB1647 : RAJI  update : 2019/10/24
CommentBurkitt's lymphoma. Sensitive to LAK cells but resistant to NK cells. Back up of culture ECA2673. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the website of ECACC prior to order.Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the website of ECACC prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 11 years
Tissue maxilla
Case history Burkitt's lymphoma
Classification cancer
History overseas, ECACC -> RIKEN Cell bank
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP, AST
Chromosome mode 46-50(50) : 46(4),47(13),48(16),49(15),50(2)
Images
deposit info
lot info
Reference information Reference 5
User's Publication 8


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Reference
2472      Trans N Y Acad Sci  1966  29:61 
972  Pulvertaft,R.J.V.  Cytology of Burkitt's tumor (African lymphoma).  Lancet  1964  1:238-240  PubMed ID: 14086209
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
479  Epstein, M A, Achong, B G, Barr, Y M, Zajac B, Henle G, Henle W  Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).  J Natl Cancer Inst  1966  37:547-59  PubMed ID: 4288580
4739  Huang CC, Minowada J.  Differential effects of infection with herpes simplex virus on the chromosomes of human hematopoietic cell lines.  Cancer Res  1972  32(6):1218-25  PubMed ID: 4337885

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User's Publication
3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080
2845  Shirakawa, Jun, Shibuya, Kazuko, Shibuya, Akira  Requirement of the serine at residue 329 for lipid raft recruitment of DNAM-1 (CD226).  Int Immunol  2005  17:217-23  PubMed ID: 15684041
3379  Kochi, Yuta, Yamada, Ryo, Suzuki, Akari, Harley, John B, Shirasawa, Senji, Sawada, Tetsuji, Bae, Sang-Cheol, Tokuhiro, Shinya, Chang, Xiaotian, Sekine, Akihiro, Takahashi, Atsushi, Tsunoda, Tatsuhiko, Ohnishi, Yozo, Kaufman, Kenneth M, Kang, Changsoo Paul, Kang, Changwon, Otsubo, Shigeru, Yumura, Wako, Mimori, Akio, Koike, Takao, Nakamura, Yusuke, Sasazuki, Takehiko, Yamamoto, Kazuhiko  A functional variant in FCRL3, encoding Fc receptor-like 3, is associated with rheumatoid arthritis and several autoimmunities.  Nat Genet  2005  37:478-85  PubMed ID: 15838509
8171  Hsum YW, Yew WT, Hong PL, Soo KK, Hoon LS, Chieng YC, Mooi LY.  Cancer chemopreventive activity of maslinic acid: suppression of COX-2 expression and inhibition of NF-κB and AP-1 activation in Raji cells.  Planta Med.  2010  77:152-7  PubMed ID: 20669087
8486  Hazawa M, Takahashi K, Wada K, Mori T, Kawahara N, Kashiwakura I.  Structure-activity relationships between the Aconitum C20-diterpenoid alkaloid derivatives and the growth suppressive activities of Non-Hodgkin's lymphoma Raji cells and human hematopoietic stem/progenitor cells.  Invest New Drugs  2009  29:1-8  PubMed ID: 19784550
8561  Takahashi-Makise N, Suzu S, Hiyoshi M, Ohsugi T, Katano H, Umezawa K, Okada S.  Biscoclaurine alkaloid cepharanthine inhibits the growth of primary effusion lymphoma in vitro and in vivo and induces apoptosis via suppression of the NF-kappaB pathway.  Int. J. Cancer  2009  125:1464-72  PubMed ID: 19521981
10269  Komohara Y, Ma C, Yano H, Pan C, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Okuno Y, Nosaka K, Shimosaki S, Morishita K, Matsuoka M, Wakayama T, Takeya M.  Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages.  J Clin Exp Hematop  2017  57:15-20  PubMed ID: 28420814
11256  Maruhashi T, Okazaki IM, Sugiura D, Takahashi S, Maeda TK, Shimizu K, Okazaki T.  LAG-3 inhibits the activation of CD4+ T cells that recognize stable pMHCII through its conformation-dependent recognition of pMHCII.  Nat. Immunol.  2018    PubMed ID: 30349037



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