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RCB1640 : DAUDI  update : 2019/10/24
CommentBurkitt's lymphoma. Sensitive to lymphokine-activated killer cells but resistant to natural killer cells. Back up culture of ECA3011. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the website of ECACC prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Race Negloid
Gender Male
Age at sampling 16 years
Tissue blood
Case history Burkitt's lymphoma
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 46-47(50) : 46(10),48(40)
deposit info
lot info
Reference information Reference 6
User's Publication 3

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2417  Nilsson K, Giovanella, B C, Stehlin, J S, Klein G  Tumorigenicity of human hematopoietic cell lines in athymic nude mice.  Int J Cancer  1977  19:337-44  PubMed ID: 14896
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
2416  Klein E, Klein G, Nadkarni, J S, Nadkarni, J J, Wigzell H, Clifford P  Surgace IgM specificity on cells derived from a Burkitt's lymphoma.  Lancet  1967  2:1068-70  PubMed ID: 4168541
971  Klein E, Klein G, Nadkarni, J S, Nadkarni, J J, Wigzell H, Clifford P  Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.  Cancer Res  1968  28:1300-10  PubMed ID: 4174339
4975  Neumann H, Klein E, Hauck-Granoth R, Yachnin S, Ben-Bassat H.  Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells.  Proc Natl Acad Sci U S A  1976  73(5):1432-6  PubMed ID: 1064015
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  ;83(20):7898-902  PubMed ID: 3464004

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User's Publication
10069  Motoshima T, Komohara Y, Ma C, Dewi AK, Noguchi H, Yamada S, Nakayama T, Kitada S, Kawano Y, Takahashi W, Sugimoto M, Takeya M, Fujimoto N, Oda Y, Eto M.  PD-L1 expression in papillary renal cell carcinoma.  BMC Urol  2017  17:8  PubMed ID: 28086852
10269  Komohara Y, Ma C, Yano H, Pan C, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Okuno Y, Nosaka K, Shimosaki S, Morishita K, Matsuoka M, Wakayama T, Takeya M.  Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages.  J Clin Exp Hematop  2017  57:15-20  PubMed ID: 28420814
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611

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