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RCB1638 : Hepa 1-6  update : 2019/10/24
CommentDerived from BW7756 tumor in a C57L mouse. Back up culture of ECA3041. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the website of ECACC prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 2000
Animal mouse < Mammals
Strain name C57L
Tissue liver
Case history hepatopma
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 22


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Reference
2679  Darlington, G J  Liver cell lines.  Methods Enzymol  1987  151:19-38  PubMed ID: 3431441
2494  Darlington, G J, Bernhard, H P, Miller, R A, Ruddle, F H  Expression of liver phenotypes in cultured mouse hepatoma cells.  J Natl Cancer Inst  1980  64:809-19  PubMed ID: 6102619

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User's Publication
3356  Kubota, Koichi  A novel functional T cell hybridoma recognizes macrophage cell death induced by bacteria: a possible role for innate lymphocytes in bacterial infection.  J Immunol  2006  176:7576-88  PubMed ID: 16751404
2637  Kasahara M, Watanabe Y, Sumasu M, Nagata T  A family of MHC class I-like genes located in the vicinity of the mouse leukocyte receptor complex.  Proc Natl Acad Sci U S A  2002  99:13687-92  PubMed ID: 12370446
7067  Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M.  Fluorescence probes to detect lipid-derived radicals.  Nat. Chem. Biol.  2016  12:608-13  PubMed ID: 27294322
7330  Uyama T, Kawai K, Kono N, Watanabe M, Tsuboi K, Inoue T, Araki N, Arai H, Ueda N.  Interaction of Phospholipase A/Acyltransferase-3 with Pex19p: A POSSIBLE INVOLVEMENT IN THE DOWN-REGULATION OF PEROXISOMES.  J. Biol. Chem.  2015  290:17520-34  PubMed ID: 26018079
7395  Mutai H, Miya F, Fujii M, Tsunoda T, Matsunaga T.  Attenuation of progressive hearing loss in DBA/2J mice by reagents that affect epigenetic modifications is associated with up-regulation of the zinc importer Zip4.  PLoS ONE  2015  10:e0124301  PubMed ID: 25875282
8206  Matsubara S, Takahashi T, Kimura AP.  Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression.  Gene  2010  465:17-29  PubMed ID: 20600704
8276  Miyayama T, Hiraoka D, Kawaji F, Nakamura E, Suzuki N, Ogra Y.  Roles of COMM-domain-containing 1 in stability and recruitment of the copper-transporting ATPase in a mouse hepatoma cell line.  Biochem. J.  2010  429:53-61  PubMed ID: 20433422
8994  Ogawa S, Tagawa Y, Kamiyoshi A, Suzuki A, Nakayama J, Hashikura Y, Miyagawa S.  Crucial roles of mesodermal cell lineages in a murine embryonic stem cell-derived in vitro liver organogenesis system.  Stem Cells  2005  23:903-13  PubMed ID: 16043458
9007  Emoto M, Miki M, Sarker AH, Nakamura T, Seki Y, Seki S, Ikeda S.  Structure and transcription promoter activity of mouse flap endonuclease 1 gene: alternative splicing and bidirectional promoter.  Gene  2005  357:47-54  PubMed ID: 15967598
9733  Takahashi T, Sugawara W, Takiguchi Y, Takizawa K, Nakabayashi A, Nakamura M, Nagano-Ito M, Ichikawa S.  Identification of Plants That Inhibit Lipid Droplet Formation in Liver Cells: Rubus suavissimus Leaf Extract Protects Mice from High-Fat Diet-Induced Fatty Liver by Directly Affecting Liver Cells.  Evid Based Complement Alternat Med  2016  2016:4282758  PubMed ID: 27429636
10033  Nishihara M, Kanda GN, Suzuki T, Yamakado S, Harashima H, Kamiya H.  Enhanced transgene expression by plasmid-specific recruitment of histone acetyltransferase.  J. Biosci. Bioeng.  2017  123:277-280  PubMed ID: 27746157
10211  Fujita K, Iguchi Y, Une M, Watanabe S.  Ursodeoxycholic Acid Suppresses Lipogenesis in Mouse Liver: Possible Role of the Decrease in β-Muricholic Acid, a Farnesoid X Receptor Antagonist.  Lipids  2017  52:335-344  PubMed ID: 28315136
10364  Lee J, Homma T, Fujii J.  Mice in the early stage of liver steatosis caused by a high fat diet are resistant to thioacetamide-induced hepatotoxicity and oxidative stress.  Toxicol. Lett.  2017  277:92-103  PubMed ID: 28642009
10525  Mizunoe Y, Kobayashi M, Sudo Y, Watanabe S, Yasukawa H, Natori D, Hoshino A, Negishi A, Okita N, Komatsu M, Higami Y.  Trehalose protects against oxidative stress by regulating the Keap1-Nrf2 and autophagy pathways.  Redox Biol  2018  15:115-124  PubMed ID: 29241092
10583  Lee J, Kang ES, Kobayashi S, Homma T, Sato H, Seo HG, Fujii J.  The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.  Exp. Cell Res.  2017  361:178-191  PubMed ID: 29079265
10795  Matsushita J, Okamura K, Nakabayashi K, Suzuki T, Horibe Y, Kawai T, Sakurai T, Yamashita S, Higami Y, Ichihara G, Hata K, Nohara K.  The DNA methylation profile of liver tumors in C3H mice and identification of differentially methylated regions involved in the regulation of tumorigenic genes.  BMC Cancer  2018  18:317  PubMed ID: 29566670
11103  Tsurudome Y, Koyanagi S, Kanemitsu T, Katamune C, Oda M, Kanado Y, Kato M, Morita A, Tahara Y, Matsunaga N, Shibata S, Ohdo S.  Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function.  Sci Rep  2018    PubMed ID: 29899468
11100  Nakano R, Ohira M, Yano T, Imaoka Y, Tanaka Y, Ohdan H.  Hepatic irradiation persistently eliminates liver resident NK cells.  PLoS ONE  2018    PubMed ID: 29897952
11157  Germond A, Ichimura T, Chiu LD, Fujita K, Watanabe TM, Fujita H.  Cell type discrimination based on image features of molecular component distribution.  Sci Rep  2018    PubMed ID: 30082723
4217  Shinto S, Matsuoka Y, Yamato M, Yamada KI.  Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death.  J Clin Biochem Nutr  2018    PubMed ID: 29610552
2705  Kushida S, Peng, B G, Uchimura E, Kuang M, Huang L, Miwa M, Ohno T  A tumour vaccine of fixed tumour fragments in a controlled-release vehicle with cytokines for therapy of hepatoma in mice.  Dig Liver Dis  2004  36:478-85  PubMed ID: 15285528
3336  Kuang M, Liu, S Q, Saijo K, Uchimura E, Huang L, Leong, K W, Lu, M D, Huang, J F, Ohno T  Microwave tumour coagulation plus in situ treatment with cytokine-microparticles: induction of potent anti-residual tumour immunity.  Int J Hyperthermia  2005  21:247-57  PubMed ID: 16019851



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