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RCB1637 : 293  update : 2018/07/06
CommentSheared human Ad5 DNA-transformed cell line. Widely used for production of manipulated adenovirus. Back up culture of ECA2737.
特性(日) ヒト胎児腎細胞由来、アデノウイルス5型による形質転換株、HEK293細胞ともいう。ECACC由来(ECA2737)株。
寄託者特性(英)
寄託者特性(日)
使用条件a
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 ECACC
寄託日 2000
別名 HEK293
動物種 human < Mammals
属名 Homo
種名 sapiens
embryo
採取組織 kidney
細胞分類 transformed
入手歴 overseas, ECACC -> Riken cell bank
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% FBS + 0.1mM NEAA
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代または培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
アイソザイム検査 LD, NP
染色体検査 63-71(50) : /65(5),66(5),67(8),68(10),69(10),70(8)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 68件
利用者成果(日) 0件

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Reference(英)
Harrison T, Graham F, Williams J  Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.  Virology  1977  77:319-29  PubMed ID: 841862
Graham, F L, Smiley J, Russell, W C, Nairn R  Characteristics of a human cell line transformed by DNA from human adenovirus type 5.  J Gen Virol  1977  36:59-74  PubMed ID: 886304

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Sugihara, Eiji, Kanai, Masayuki, Saito, Soichiro, Nitta, Takayuki, Toyoshima, Hideo, Nakayama, Keiko, Nakayama, Keiichi I, Fukasawa, Kenji, Schwab, Manfred, Saya, Hideyuki, Miwa, Masanao  Suppression of centrosome amplification after DNA damage depends on p27 accumulation.  Cancer Res  2006  66:4020-9 
Ikeno Y, Konno N, Cheon SH, Bolchi A, Ottonello S, Kitamoto K, Arioka M  Secretory phospholipases A2 induce neurite outgrowth in PC12 cells through lysophosphatidylcholine generation and activation of G2A receptor.  J Biol Chem  2005  280:28044-52 
Nagayoshi, Ryusaku, Nagai, Taku, Matsushita, Kakushi, Sato, Katsuaki, Sunahara, Nobuhiko, Matsuda, Takemasa, Nakamura, Tadashi, Komiya, Setsuro, Onda, Masanori, Matsuyama, Takami  Effectiveness of anti-folate receptor beta antibody conjugated with truncated Pseudomonas exotoxin in the targeting of rheumatoid arthritis synovial macrophages.  Arthritis Rheum  2005  52:2666-75 
Uchida S, Yoshinaga N, Yanagihara K, Yuba E, Kataoka K, Itaka K.  Designing immunostimulatory double stranded messenger RNA with maintained translational activity through hybridization with poly A sequences for effective vaccination.  Biomaterials  2017  150:162-170  PubMed ID: 29031816
Funato H, Miyoshi C, Fujiyama T, Kanda T, Sato M, Wang Z, Ma J, Nakane S, Tomita J, Ikkyu A, Kakizaki M, Hotta-Hirashima N, Kanno S, Komiya H, Asano F, Honda T, Kim SJ, Harano K, Muramoto H, Yonezawa T, Mizuno S, Miyazaki S, Connor L, Kumar V, Miura I, Suzuki T, Watanabe A, Abe M, Sugiyama F, Takahashi S, Sakimura K, Hayashi Y, Liu Q, Kume K, Wakana S, Takahashi JS, Yanagisawa M.  Forward-genetics analysis of sleep in randomly mutagenized mice.  Nature  2016  539:378-383  PubMed ID: 27806374
Mimura Y, Kelly RM, Unwin L, Albrecht S, Jefferis R, Goodall M, Mizukami Y, Mimura-Kimura Y, Matsumoto T, Ueoka H, Rudd PM.  Enhanced sialylation of a human chimeric IgG1 variant produced in human and rodent cell lines.  J. Immunol. Methods  2015  428:30-6  PubMed ID: 26627984
Sato Y, Sasaki T, Takahashi S, Kumagai T, Nagata K.  Development of a highly reproducible system to evaluate inhibition of cytochrome P450 3A4 activity by natural medicines.  J Pharm Pharm Sci  2015  18:316-27  PubMed ID: 26626238
Shizu R, Abe T, Benoki S, Takahashi M, Kodama S, Miayata M, Matsuzawa A, Yoshinari K.  PXR stimulates growth factor-mediated hepatocyte proliferation by cross-talk with the FOXO transcription factor.  Biochem. J.  2015  473:257-66  PubMed ID: 26574435
Shimizu S, Yonezawa R, Negoro T, Yamamoto S, Numata T, Ishii M, Mori Y, Toda T.  Sensitization of H2O2-induced TRPM2 activation and subsequent interleukin-8 (CXCL8) production by intracellular Fe(2+) in human monocytic U937 cells.  Int. J. Biochem. Cell Biol.  2015  68:119-27  PubMed ID: 26386353
Nagahama M, Seike S, Shirai H, Takagishi T, Kobayashi K, Takehara M, Sakurai J.  Role of P2X7 receptor in Clostridium perfringens beta-toxin-mediated cellular injury.  Biochim. Biophys. Acta  2015  1850:2159-67  PubMed ID: 26299247
Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T.  Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.  Nat Commun  2015  6:8021  PubMed ID: 26282514
Miura R, Kooriyama T, Yoneda M, Takenaka A, Doki M, Goto Y, Sanjoba C, Endo Y, Fujiyuki T, Sugai A, Tsukiyama-Kohara K, Matsumoto Y, Sato H, Kai C.  Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs.  PLoS Negl Trop Dis  2015  9:e0003914  PubMed ID: 26162094
Ito M, Shichita T, Okada M, Komine R, Noguchi Y, Yoshimura A, Morita R.  Bruton's tyrosine kinase is essential for NLRP3 inflammasome activation and contributes to ischaemic brain injury.  Nat Commun  2015  6:7360  PubMed ID: 26059659
Kato T, Yui M, Deo VK, Park EY.  Development of Rous sarcoma Virus-like Particles Displaying hCC49 scFv for Specific Targeted Drug Delivery to Human Colon Carcinoma Cells.  Pharm. Res.  2015  32:3699-707  PubMed ID: 26047779
Nakazato R, Takarada T, Ikeno S, Nakamura S, Kutsukake T, Hinoi E, Yoneda Y.  Upregulation of Runt-Related Transcription Factor-2 Through CCAAT Enhancer Binding Protein-β Signaling Pathway in Microglial BV-2 Cells Exposed to ATP.  J. Cell. Physiol.  2015  230:2510-21  PubMed ID: 25802132
Ono A, Ito A, Suzuki T, Yamaguchi M, Kawabe Y, Kamihira M.  DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification.  J. Biosci. Bioeng.  2015  120:463-6  PubMed ID: 25772439
Kamada Y, Kusakabe T, Sugimoto Y.  Amyloidogenic lysozymes accumulate in the endoplasmic reticulum accompanied by the augmentation of ER stress signals.  Biochim. Biophys. Acta  2015  1850:1107-19  PubMed ID: 25659958
Tamura M, Kunihiro S, Hamashima Y, Yoshioka Y, Tone S, Kameda K.  An improved superoxide-generating nanodevice for oxidative stress studies in cultured cells.  Biotechnology Reports  2015  6:45-50 
Gono T, Takarada T, Fukumori R, Kawaguchi Y, Kaneko H, Hanaoka M, Katsumata Y, Yoneda Y, Yamanaka H.  NR2-reactive antibody decreases cell viability through augmentation of Ca(2+) influx in systemic lupus erythematosus.  Arthritis Rheum.  2011  63:3952-9  PubMed ID: 22012858
Chang KL, Higuchi Y, Kawakami S, Yamashita F, Hashida M.  Development of lysine-histidine dendron modified chitosan for improving transfection efficiency in HEK293 cells.  J Control Release  2011  156:195-202  PubMed ID: 21802461
Takahashi Y, Shimokawa N, Esmaeili-Mahani S, Morita A, Masuda H, Iwasaki T, Tamura J, Haglund K, Koibuchi N.  Ligand-induced downregulation of TrkA is partly regulated through ubiquitination by Cbl.  FEBS Lett.  2011  585:1741-7  PubMed ID: 21570973
Watanabe M, Itoh K.  Characterization of a novel posttranslational modification in neuronal nitric oxide synthase by small ubiquitin-related modifier-1.  Biochim. Biophys. Acta  2011  1814:900-7  PubMed ID: 21545853
Okano I, Miyazato M, Kangawa K.  A guanosine 3',5'-cyclic monophosphate (cGMP) reporter system based on the G-kinase/CREB/CRE signal transduction pathway.  Biochem. Biophys. Res. Commun.  2011  407:236-41  PubMed ID: 21382339
Jin ZB, Okamoto S, Osakada F, Homma K, Assawachananont J, Hirami Y, Iwata T, Takahashi M.  Modeling retinal degeneration using patient-specific induced pluripotent stem cells.  PLoS ONE  2011  6:e17084  PubMed ID: 21347327
Ohtani M, Miyadai T.  Functional analysis of fish BCL-6 and Blimp-1 in vitro: transcriptional repressors for B-cell terminal differentiation in fugu (Takifugu rubripes).  Mol. Immunol.  2011  48:818-25  PubMed ID: 21216469
Kohno M, Horibe T, Haramoto M, Yano Y, Ohara K, Nakajima O, Matsuzaki K, Kawakami K.  A novel hybrid peptide targeting EGFR-expressing cancers.  Eur. J. Cancer  2010  47:773-83  PubMed ID: 21112771
Sugimoto M, Sasaki S, Watanabe T, Nishimura S, Ideta A, Yamazaki M, Matsuda K, Yuzaki M, Sakimura K, Aoyagi Y, Sugimoto Y.  Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate.  PLoS ONE  2010  5:e13817  PubMed ID: 21072200
Hashimoto O, Yonezawa T, Sugiyama Y, Kawaminami M, Hasegawa Y.  Molecular cloning and expression of canine prolactin gene.  Exp. Anim.  2010  59:643-6  PubMed ID: 21030793
Kido S, Kuriwaka-Kido R, Umino-Miyatani Y, Endo I, Inoue D, Taniguchi H, Inoue Y, Imamura T, Matsumoto T.  Mechanical stress activates Smad pathway through PKCδ to enhance interleukin-11 gene transcription in osteoblasts.  PLoS ONE  2010  5:  PubMed ID: 20927330
Ishihara K, Okuyama S, Kumano S, Iida K, Hamana H, Murakoshi M, Kobayashi T, Usami S, Ikeda K, Haga Y, Tsumoto K, Nakamura H, Hirasawa N, Wada H.  Salicylate restores transport function and anion exchanger activity of missense pendrin mutations.  Hear. Res.  2010  270:110-8  PubMed ID: 20826203
Fukumori R, Takarada T, Nakamichi N, Kambe Y, Kawagoe H, Nakazato R, Yoneda Y.  Requirement of both NR3A and NR3B subunits for dominant negative properties on Ca2+ mobilization mediated by acquired N-methyl-D-aspartate receptor channels into mitochondria.  Neurochem. Int.  2010  57:730-7  PubMed ID: 20813147
Kotani H, Tanabe H, Mizukami H, Makishima M, Inoue M.  Identification of a naturally occurring rexinoid, honokiol, that activates the retinoid X receptor.  J. Nat. Prod.  2010  73:1332-6  PubMed ID: 20695472
Chang KL, Higuchi Y, Kawakami S, Yamashita F, Hashida M.  Efficient gene transfection by histidine-modified chitosan through enhancement of endosomal escape.  Bioconjug. Chem.  2010  21:1087-95  PubMed ID: 20499901
Yokoi H, Mizukami H, Nagatsu A, Tanabe H, Inoue M.  Hydroxy monounsaturated fatty acids as agonists for peroxisome proliferator-activated receptors.  Biol. Pharm. Bull.  2010  33:854-61  PubMed ID: 20460766
Feng GG, Li C, Huang L, Tsunekawa K, Sato Y, Fujiwara Y, Komatsu T, Honda T, Fan JH, Goto H, Koide T, Hasegawa T, Ishikawa N.  Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor necrosis factor-induced apoptosis.  Biochem. Biophys. Res. Commun.  2010  394:153-7  PubMed ID: 20193664
Ikari A, Sanada A, Okude C, Sawada H, Yamazaki Y, Sugatani J, Miwa M.  Up-regulation of TRPM6 transcriptional activity by AP-1 in renal epithelial cells.  J. Cell. Physiol.  2009  222:481-7  PubMed ID: 19937979
Ishii M, Kobayashi S, Ohkura M, Yamamoto R, Shimizu S, Kiuchi Y.  Inhibitory effect of lomerizine, a prophylactic drug for migraines, on serotonin-induced contraction of the basilar artery.  J. Pharmacol. Sci.  2009  111:221-5  PubMed ID: 19783864
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K.  Equatorin: identification and characterization of the epitope of the MN9 antibody in the mouse.  Biol. Reprod.  2009  81:889-97  PubMed ID: 19605790
Munekata K, Sakamoto K.  Forkhead transcription factor Foxo1 is essential for adipocyte differentiation.  In Vitro Cell. Dev. Biol. Anim.  2009  45:642-51  PubMed ID: 19585174
Idogawa M, Sasaki Y, Suzuki H, Mita H, Imai K, Shinomura Y, Tokino T.  A single recombinant adenovirus expressing p53 and p21-targeting artificial microRNAs efficiently induces apoptosis in human cancer cells.  Clin. Cancer Res.  2009  15:3725-32  PubMed ID: 19458054
Takarada T, Takahata Y, Iemata M, Hinoi E, Uno K, Hirai T, Yamamoto T, Yoneda Y.  Interference with cellular differentiation by D-serine through antagonism at N-methyl-D-aspartate receptors composed of NR1 and NR3A subunits in chondrocytes.  J. Cell. Physiol.  2009  220:756-64  PubMed ID: 19452450
Takarada T, Hojo H, Iemata M, Sahara K, Kodama A, Nakamura N, Hinoi E, Yoneda Y.  Interference by adrenaline with chondrogenic differentiation through suppression of gene transactivation mediated by Sox9 family members.  Bone  2009  45:568-78  PubMed ID: 19446054
Shinkai Y, Sumi D, Toyama T, Kaji T, Kumagai Y.  Role of aquaporin 9 in cellular accumulation of arsenic and its cytotoxicity in primary mouse hepatocytes.  Toxicol. Appl. Pharmacol.  2009  237:232-6  PubMed ID: 19341753
Miki Y, Itoh T, Hirano K, Eda S, Hayashi A, Yamanaka M, Beppu M.  Clearance of oxidatively damaged cells by macrophages: recognition of glycoprotein clusters by macrophage-surface nucleolin as early apoptotic cells.  Biol. Pharm. Bull.  2009  32:564-72  PubMed ID: 19336885
Uehara Y, Mochizuki M, Matsuno K, Haino T, Asai A.  Novel high-throughput screening system for identifying STAT3-SH2 antagonists.  Biochem. Biophys. Res. Commun.  2009  380:627-31  PubMed ID: 19285012
Okamura H, Amorim BR, Wang J, Yoshida K, Haneji T.  Calcineurin regulates phosphorylation status of transcription factor osterix.  Biochem. Biophys. Res. Commun.  2008  379:440-4  PubMed ID: 19114031
Suzuki T, Sakurai F, Nakamura S, Kouyama E, Kawabata K, Kondoh M, Yagi K, Mizuguchi H.  miR-122a-regulated expression of a suicide gene prevents hepatotoxicity without altering antitumor effects in suicide gene therapy.  Mol. Ther.  2008  16:1719-26  PubMed ID: 18665157
Tao Y, Nomura M, Kitabatake N, Tani F.  Mouse CD40-transfected cell lines cannot exhibit the binding and RANTES-stimulating activity of exogenous heat shock protein 70.  Mol. Immunol.  2006  44:1262-73  PubMed ID: 16930707
Kubo Y, Akiyama M, Fukada Y, Okano T.  Molecular cloning, mRNA expression, and immunocytochemical localization of a putative blue-light photoreceptor CRY4 in the chicken pineal gland.  J. Neurochem.  2006  97:1155-65  PubMed ID: 16686694
Sugihara E, Kanai M, Saito S, Nitta T, Toyoshima H, Nakayama K, Nakayama KI, Fukasawa K, Schwab M, Saya H, Miwa M.  Suppression of centrosome amplification after DNA damage depends on p27 accumulation.  Cancer Res.  2006  66:4020-9  PubMed ID: 16618721
Matsui K, Horiuchi S, Sando S, Sera T, Aoyama Y.  RNAi silencing of exogenous and endogenous reporter genes using a macrocyclic octaamine as a "compact" siRNA carrier. Studies on the nonsilenced residual activity.  Bioconjug. Chem.  2006  17:132-8  PubMed ID: 16417261
Nagayoshi R, Nagai T, Matsushita K, Sato K, Sunahara N, Matsuda T, Nakamura T, Komiya S, Onda M, Matsuyama T.  Effectiveness of anti-folate receptor beta antibody conjugated with truncated Pseudomonas exotoxin in the targeting of rheumatoid arthritis synovial macrophages.  Arthritis Rheum.  2005  52:2666-75  PubMed ID: 16142741
Ariyasu T, Matsumoto S, Kyono F, Hanaya T, Arai S, Ikeda M, Kurimoto M.  Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose.  In Vitro Cell. Dev. Biol. Anim.  2003  39:80-8  PubMed ID: 12892531
Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781
Toda T, Yamamoto S, Yonezawa R, Mori Y, Shimizu S.  Inhibitory effects of Tyrphostin AG-related compounds on oxidative stress-sensitive transient receptor potential channel activation.  Eur. J. Pharmacol.  2016  786:19-28  PubMed ID: 27238971
Tohno M, Shinkai H, Toki D, Okumura N, Tajima K, Uenishi H.  Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.  Immunogenetics  2016  68:693-701  PubMed ID: 27236661
Moriwaki S, Into T, Suzuki K, Miyauchi M, Takata T, Shibayama K, Niida S.  γ-Glutamyltranspeptidase is an endogenous activator of Toll-like receptor 4-mediated osteoclastogenesis.  Sci Rep  2016  6:35930  PubMed ID: 27775020
Furukawa C, Fujii N, Manabe A, Matsunaga T, Endo S, Hasegawa H, Ito Y, Yamaguchi M, Yamazaki Y, Ikari A.  Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Expression by Tumor Necrosis Factor-α in the Presence of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor.  J. Cell. Physiol.  2017  232:2841-2850  PubMed ID: 27925186
Imaizumi Y, Goda T, Schaffhauser DF, Okada JI, Matsumoto A, Miyahara Y.  Proton-sensing transistor systems for detecting ion leakage from plasma membranes under chemical stimuli.  Acta Biomater  2017  50:502-509  PubMed ID: 27956364
Moriwaki S, Into T, Suzuki K, Miyauchi M, Takata T, Shibayama K, Niida S.  γ-Glutamyltranspeptidase is an endogenous activator of Toll-like receptor 4-mediated osteoclastogenesis.  Sci Rep  2016  6:35930  PubMed ID: 27775020
Satou K, Mochimaru Y, Nakakura T, Kusada T, Negishi J, Musha S, Yoshimura N, Kato Y, Tomura H.  Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase.  J. Reprod. Dev.  2017  63:199-204  PubMed ID: 28090002
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Endo-Umeda K, Aoyama A, Shimizu M, Ishikawa M, Hashimoto Y, Yamada S, Makishima M.  1α-Hydroxy derivatives of 7-dehydrocholesterol are selective liver X receptor modulators.  J. Steroid Biochem. Mol. Biol.  2017  172:136-148  PubMed ID: 28736297
Kitamura H, Ishino T, Shimamoto Y, Okabe J, Miyamoto T, Takahashi E, Miyoshi I.  Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells.  Mediators Inflamm.  2017  2017:6909415  PubMed ID: 29138532
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Nakamura M, Fujita K, Toyoda Y, Takada T, Hasegawa H, Ichida K.  Investigation of the transport of xanthine dehydrogenase inhibitors by the urate transporter ABCG2.  Drug Metab. Pharmacokinet.  2018  33:77-81  PubMed ID: 29342419
  One-pot Synthesis of Water Soluble Highly Fluorescent Silica Nanoparticles        PubMed ID:

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