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RCB1635 : K-562  update : 2018/07/06
CommentHuman CML derived cell line. Deposited directly from the institution that has established this cell.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ichiki, T. Albert
Originator Lozzio, C.B. & Lozzio, B.B.
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Case history chrinic myelogenous leukemia
Classification cancer
History overseas
Lifespan infinite
Morphology lymphoblast-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives MEM + 15% FBS + 0.1mM NEAA
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5 x 10^(4) cells/ml
Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 5% Glycerol
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 62-67(50) : /63(3),64(8),65(33),66(4),67(1)
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  ;83(20):7898-902  PubMed ID: 3464004
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644

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