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RCB1591 : SRA 01/04  update : 2018/07/06
Commentalpha, beta-crystaline expressing human lense epithelial cell line. plasmid DNA: pRSV-B-T Ag.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ibaraki, Nobuhiro
Originator Ibaraki, Nobuhiro
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 3 months
Tissue eye, lens
Classification transformed
Recombinant recombinant
Exogene pRSV-B-T Ag
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 20% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Chromosome mode 55-60(50) : 55(2),56(9),57(12),58(15),59(10),60(2)
Images
deposit info
lot info
Reference information Reference 4
User's Publication 4


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Reference
2409  Ibaraki N, Chen, S C, Lin, L R, Okamoto H, Pipas, J M, Reddy, V N  Human Lens Epithelial Cell Line  Exp Cell Res  1998  67:577-585  PubMed ID: 9878220
4969  Oharazawa H, Ibaraki N, Lin LR, Reddy VN.  The effects of extracellular matrix on cell attachment, proliferation and migration in a human lens epithelial cell line.  Exp Eye Res  1999  69(6):603-10  PubMed ID: 10620389
4970  Oharazawa H, Ibaraki N, Ohara K, Reddy VN.  Inhibitory effects of Arg-Gly-Asp (RGD) peptide on cell attachment and migration in a human lens epithelial cell line.  Ophthalmic Res  2005  37(4):191-6  PubMed ID: 15990462
4971  Yamashiro Y1, Sasaki H, Ibaraki N, Nagai K, Kawakami Y, Yaguchi H, Fujita N, Osada H, Sasaki K.  Cyclin-dependent kinase inhibitor p16 and p21 expression, and cell cycle change in human lens epithelial cell line SRA 01/04 following contact inhibition in normal culture.  Ophthalmic Res  2011  46(1):38-43  PubMed ID: 21212707

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User's Publication
8076  Tang X, Yao K, Zhang L, Yang Y, Yao H.  Honokiol inhibits H(2)O(2)-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways.  Eur. J. Pharmacol.  2010  650:72-8  PubMed ID: 20965163
9519  Zhu K, Lv Y, Cheng Q, Hua J, Zeng Q.  Extremely Low Frequency Magnetic Fields Do Not Induce DNA Damage in Human Lens Epithelial Cells In Vitro.  Anat Rec (Hoboken)  2016  299:688-97  PubMed ID: 27079842
10720  Shentu XC, Ping XY, Cheng YL, Zhang X, Tang YL, Tang XJ.  Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide.  Int J Ophthalmol  2018  11:12-17  PubMed ID: 29375984
11257  Yu X, Ping X, Zhang X, Cui Y, Yang H, Tang X, Tang Y, Shentu X.  The impact of GJA8 SNPs on susceptibility to age-related cataract.  Hum. Genet.  2018    PubMed ID: 30349978



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