細胞番号 : 細胞名
RCB1538 : 32Dcl3
update : 2024/01/17
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| 細胞特性(Comment:英) | Subclone of 32D cell line. Differentiate to mature granulocytes by G-CSF. Conditioned medium. |
| 細胞特性(日) | 32D細胞亜株。G-CSFにより成熟顆粒球に分化する。WEHI-3細胞のConditioned mediumを必要とする。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Kamesaki, Hiroshi
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| 寄託日 |
1999
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| 元の細胞 |
32D
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| 動物種 |
_mouse
< Mammals
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| 動物系統名 |
C3H/HeJ
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| 採取組織 |
bone marrow
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| 病名 |
myeloid precursor cell
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| 細胞分類 |
transformed
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|
細胞寿命 |
infinite
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| 細胞形態 |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_0119
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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| 培地 |
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IMDM (4mM L-Glutamine) + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium)
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| 培養方法 |
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浮遊細胞の培養に関する一般的な注意
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| 抗生物質 |
|
Free
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|
継代方法 |
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dilution
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|
継代密度 |
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1 : 5 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
|
37
℃
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| 二酸化炭素濃度 |
|
5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| マウス系統検査 |
|
OK
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| アイソザイム検査 |
|
LD, NP
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
9件
|
| Reference(日) |
0件
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| 利用者成果(英) |
5件
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| 利用者成果(日) |
0件
|
| Reference(英) |
|
5470
Patel G, Kreider B, Rovera G, Reddy EP.
v-myb blocks granulocyte colony-stimulating factor-induced myeloid cell differentiation but not proliferation.
Mol Cell Biol
1993
13(4):2269-76
PubMed ID: 7681145
DOI: 10.1128/mcb.13.4.2269
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2259
Kreider BL, Benezra R, Rovera G, Kadesch T.
Inhibition of myeloid differentiation by the helix-loop-helix protein Id.
Science
1992
255(5052):1700-2
PubMed ID: 1372755
DOI: 10.1126/science.1372755
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5468
Friedman AD, Krieder BL, Venturelli D, Rovera G.
Transcriptional regulation of two myeloid-specific genes, myeloperoxidase and lactoferrin, during differentiation of the murine cell line 32D C13.
Blood
1991
78(9):2426-32
PubMed ID: 1718497
|
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2260
Kreider BL, Phillips PD, Prystowsky MB, Shirsat N, Pierce JH, Tushinski R, Rovera G.
Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell.
Mol Cell Biol
1990
10(9):4846-53
PubMed ID: 1697033
DOI: 10.1128/mcb.10.9.4846
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5466
Migliaccio G, Migliaccio AR, Kreider BL, Rovera G, Adamson JW.
Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.
J Cell Biol
1989
109(2):833-41
PubMed ID: 2668305
DOI: 10.1083/jcb.109.2.833
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4924
Tweardy DJ1, Caracciolo D, Valtieri M, Rovera G.
Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.
Ann N Y Acad Sci
1987
511:30-8
PubMed ID: 2830826
DOI: 10.1111/j.1749-6632.1987.tb36235.x
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4925
Rovera G, Valtieri M, Mavilio F, Reddy EP.
Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line.
Oncogene
1987
1(1):29-35
PubMed ID: 2449644
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2770
Valtieri M, Tweardy DJ, Caracciolo D, Johnson K, Mavilio F, Altmann S, Santoli D, Rovera G.
Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.
J Immunol
1987
138(11):3829-35
PubMed ID: 2438328
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|
1979
Greenberger, J S, Sakakeeny, M A, Humphries, R K, Eaves, C J, Eckner, R J
Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.
Proc Natl Acad Sci U S A
1983
80:2931-5
PubMed ID: 6574462
DOI: 10.1073/pnas.80.10.2931
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| 利用者成果(英) |
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4869
Kitagawa J, Hara T, Tsurumi H, Kanemura N, Kasahara S, Shimizu M, Moriwaki H.
Cell cycle-dependent priming action of granulocyte colony-stimulating factor (G-CSF) enhances in vitro apoptosis induction by cytarabine and etoposide in leukemia cell lines.
J Clin Exp Hematop
2010
50(2):99-105
PubMed ID: 21123967
DOI: 10.3960/jslrt.50.99
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18476
Aoki N, Kimura Y, Kimura S, Nagato T, Azumi M, Kobayashi H, Sato K, Tateno M.
Expression and functional role of MDL-1 (CLEC5A) in mouse myeloid lineage cells
J Leukoc Biol
2009
85(3):508-17
PubMed ID: 19074552
DOI: 10.1189/jlb.0508329
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18167
Jun-ichi Kitagawa, Takeshi Hara, Hisashi Tsurumi, Nobuhiro Kanemura, Masahito Shimizu, Hisataka Moriwai
Priming Effect with G-CSF Enhances In Vitro Apoptosis by Treatment with Ara-C and VP-16 in Leukemic Cell Line
Blood
2007
110:4333-4333
DOI: 10.1182/blood.v110.11.4333.4333
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18674
Yamaguchi M, Fujimura K, Toga H, Khwaja A, Okamura N, Chopra R.
Shwachman-Diamond syndrome is not necessary for the terminal maturation of neutrophils but is important for maintaining viability of granulocyte precursors
Exp Hematol
2007
35(4):579-86
PubMed ID: 17379068
DOI: 10.1016/j.exphem.2006.12.010
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4892
Kumagai H1, Oki T, Tamitsu K, Feng SZ, Ono M, Nakajima H, Bao YC, Kawakami Y, Nagayoshi K, Copeland NG, Gilbert DJ, Jenkins NA, Kawakami T, Kitamura T.
Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells.
Biochem Biophys Res Commun
2003
307(3):719-29
PubMed ID: 12893283
DOI: 10.1016/s0006-291x(03)01245-2
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