Cell No. : Cell Name
RCB1537 : MLC-7
update : 2022/04/05
|
| Comment | Bone marrow stroma cell line, depositing calcified matrix on the culture surface. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Sakiyama, Hisako
|
| Originator |
Sakiyama, Hisako
|
| Year of deposit |
1999
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/He
|
| Tissue |
bone marrow
|
| Classification |
semi-normal
|
| Year of origin |
1994
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_2I00
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
McCoy's 5A + 20% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
pipetting
|
Culture information
|
Passage cell No |
|
1 x 10 6 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP, PepB
|
| Reference information |
Reference |
0
|
| User's Publication |
2
|
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