Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1514 : HOCE  update : 2023/05/24
CommentStopped distribution due to the limited proliferation capacity. Human cell line derived from choriocarcinoma. Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Ishiwata Obstetrics & Gynecology Hospital
1-4-21 Kami-mito, Mito-city 310-0041, Japan
Dr.ISHIWATA Isamu
Fax. +81-29-221-1012



English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, I. & Ishikawa, H.
Year of deposit 1999
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 34 years
Tissue uterine
Disease name choriocarcinoma with pregnancy
Classification cancer
Year of origin 1995
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4840
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin or (0.25% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 2-8 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 61-83(50) : /77(4), 78(8), 79(5), 80(7), 81(9)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


To topTop
Reference

To topTop
User's Publication



Back Back Return Top Page