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RCB1512 : MH7A  update : 2018/07/06
CommentRheumatoid fibroblast-like synoviocyte transformed with SV40 T antigen. Respond to IL-1beta.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Kissei Pharmaceutical Co., Ltd.

4365-1 Kashihara, Hodaka, Azumino, Nagano 399-8304 Japan
Dr.R & D, Planning
Fax. +81-263-82-8826
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor R & D, Planning
Originator Miyazawa, Keiji
Year of deposit 1999
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 53 years
Tissue synovial
Case history Rheumatoid Arthritis (RA)
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 66-82(50) : /73(6),74(4),75(6),76(3),77(7),78(5)/
deposit info
lot info
Reference information Reference 2
User's Publication 26

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2339  Miyazawa K, Mori A, Yamamoto K, Okudaira H  Transcriptional roles of CCAAT/enhancer binding protein-beta, nuclear factor-kappaB, and C-promoter binding factor 1 in interleukin (IL)-1beta-induced IL-6 synthesis by human rheumatoid fibroblast-like synoviocytes.  J Biol Chem  1998  273:7620-7  PubMed ID: 9516466
2338  Miyazawa K, Mori A, Okudaira H  Establishment and Characterization of a Novel Human Rheumatoid Fibroblast-Like Synoviocyte Line, MH7A, Immortalized with SV40 T Antigen  J Biochem  1998  124:1153-1162  PubMed ID: 9832620

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User's Publication
11298  Ma J, Wang X, Lv T, Liu J, Ren Y, Zhang J, Zhang Y.  Effects of Ghrelin on the Apoptosis of Rheumatoid Arthritis Fibroblast-Like Synoviocyte MH7A Cells.  Biol. Pharm. Bull.  2019    PubMed ID: 30429411
11434  Hayakawa K, Kawasaki M, Hirai T, Yoshida Y, Tsushima H, Fujishiro M, Ikeda K, Morimoto S, Takamori K, Sekigawa I.  MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-κB Signaling.  Int J Mol Sci  2019    PubMed ID: 30769772
11529  Meng D, Li J, Li H, Wang K.  Salvianolic acid B remits LPS-induced injury by up-regulating miR-142-3p in MH7A cells.  Biomed. Pharmacother.  2019    PubMed ID: 31022596
11688  Wen-Xiang Cheng, Huan Huang, Jian-Hai Chen, Tian-Tian Zhang, Guo-Yuan Zhu, Zheng-Tan Zheng, Jie-Tao Lin, Yi-Ping Hu, Yong Zhang, Xue-Ling Bai, Yan Wang, Zhan-Wang Xu, Bing Song, Yi-Ying Mao, Fei Yang, Peng Zhang  Genistein inhibits angiogenesis developed during rheumatoid arthritis through the IL-6/JAK2/STAT3/VEGF signalling pathway  Journal of Orthopaedic Translation  2019   
10840  Jia W, Wu W, Yang D, Xiao C, Huang M, Long F, Su Z, Qin M, Liu X, Zhu YZ.  GATA4 regulates angiogenesis and persistence of inflammation in rheumatoid arthritis.  Cell Death Dis  2018  9:503  PubMed ID: 29717129
11091  Takeno M, Kitagawa S, Yamanaka J, Teramoto M, Tomita H, Shirai N, Itoh S, Hida S, Hayakawa K, Onozaki K, Takii T.  5-Hydroxy-2-methylpyridine Isolated from Cigarette Smoke Condensate Aggravates Collagen-Induced Arthritis in Mice.  Biol. Pharm. Bull.  2018  41(6):877-884  PubMed ID: 29863076
11169  Wang X, Wang X, Sun J, Fu S.  An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome?protamine-DNA-siRNA complex with cell permeable peptides.  Int. J. Mol. Med.  2018  42(5):2393-2402  PubMed ID: 30106091
11203  Zhong F, Xu J, Yang X, Zhang Q, Gao Z, Deng Y, Zhang L, Yu C.  miR-145 eliminates lipopolysaccharides-induced inflammatory injury in human fibroblast-like synoviocyte MH7A cells.  J. Cell. Biochem.  2018    PubMed ID: 30191608
4296  He L, Liu C, Sun C, Wang J, Zhi K, Sun D, Wang H, Wang Q, Lin N.  Wu-Tou Decoction Inhibits Angiogenesis in Experimental Arthritis by Targeting VEGFR2 Signaling Pathway.  Rejuvenation Res  2018    PubMed ID: 29385909
4297  Wu J, Fan W, Ma L, Geng X.  miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/β-catenin pathway.  Drug Des Devel Ther  2018    PubMed ID: 30349197
10252  Lee JW, Lee J, Um SH, Moon EY.  Synovial cell death is regulated by TNF-α-induced expression of B-cell activating factor through an ERK-dependent increase in hypoxia-inducible factor-1α.  Cell Death Dis  2017  8:e2727  PubMed ID: 28383556
10404  Tsushima H, Morimoto S, Fujishiro M, Yoshida Y, Hayakawa K, Hirai T, Miyashita T, Ikeda K, Yamaji K, Takamori K, Takasaki Y, Sekigawa I, Tamura N.  Kinase inhibitors of the IGF-1R as a potential therapeutic agent for rheumatoid arthritis.  Autoimmunity  2017  50:329-335  PubMed ID: 28682648
10932    Role of SNF5 in rheumatoid arthritis by up-regulation of p16 and inactivation of JNK 1 pathway  Turkish Journal of Biology  2017  41: 535-542 
9866  Wu WJ, Jia WW, Liu XH, Pan LL, Zhang QY, Yang D, Shen XY, Liu L, Zhu YZ.  S-propargyl-cysteine attenuates inflammatory response in rheumatoid arthritis by modulating the Nrf2-ARE signaling pathway.  Redox Biol  2016  10:157-167  PubMed ID: 27744121
9879  Someya A, Ikegami T, Sakamoto K, Nagaoka I.  Glucosamine Downregulates the IL-1β-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms.  PLoS ONE  2016  11:e0165158  PubMed ID: 27776166
9157  Seiji Shibasaki, Miki Karasaki, Shunsuke Aburaya, Hironobu Morisaka, Yumiko Takeda, Wataru Aoki, Sachie Kitano, Masayasu Kitano, Mitsuyoshi Ueda, Hajime Sano, Tsuyoshi Iwasaki  A Comparative Proteomics Study of a Synovial Cell Line Stimulated With TNF-α  FEBS Open Bio  2016  6 (5), 418-24  PubMed ID: 27419047
7143  Imamura H, Yoshina S, Ikari K, Miyazawa K, Momohara S, Mitani S.  Impaired NFKBIE gene function decreases cellular uptake of methotrexate by down-regulating SLC19A1 expression in a human rheumatoid arthritis cell line.  Mod Rheumatol  2015  26:507-16  PubMed ID: 26587663
7144  Miyashita T, Morimoto S, Fujishiro M, Hayakawa K, Suzuki S, Ikeda K, Miyazawa K, Morioka M, Takamori K, Ogawa H, Sekigawa I, Takasaki Y.  Inhibition of each module of connective tissue growth factor as a potential therapeutic target for rheumatoid arthritis.  Autoimmunity  2015  49:109-14  PubMed ID: 26584911
7188  Jia Q, Cheng W, Yue Y, Hu Y, Zhang J, Pan X, Xu Z, Zhang P.  Cucurbitacin E inhibits TNF-α-induced inflammatory cytokine production in human synoviocyte MH7A cells via suppression of PI3K/Akt/NF-κB pathways.  Int. Immunopharmacol.  2015  29:884-890  PubMed ID: 26453509
7598  Fujishiro M, Nozawa K, Kawasaki M, Yamaguchi A, Iwabuchi K, Yanagida M, Suzuki F, Miyazawa K, Fukui H, Kaneko K, Ogawa H, Takamori K, Takasaki Y, Sekigawa I.  Regenerating gene (REG) 1 alpha promotes pannus progression in patients with rheumatoid arthritis.  Mod Rheumatol  2011  22:228-37  PubMed ID: 22203215
7631  Igarashi H, Yahagi A, Saika T, Hashimoto J, Tomita T, Yoshikawa H, Ishihara K.  A pro-inflammatory role for A20 and ABIN family proteins in human fibroblast-like synoviocytes in rheumatoid arthritis.  Immunol. Lett.  2011  141:246-53  PubMed ID: 22093807
7779  Seong AR, Yoo JY, Choi K, Lee MH, Lee YH, Lee J, Jun W, Kim S, Yoon HG.  Delphinidin, a specific inhibitor of histone acetyltransferase, suppresses inflammatory signaling via prevention of NF-κB acetylation in fibroblast-like synoviocyte MH7A cells.  Biochem. Biophys. Res. Commun.  2011  410:581-6  PubMed ID: 21683061
7800  Tanaka Y, Matsumoto I, Iwanami K, Inoue A, Minami R, Umeda N, Kanamori A, Ochiai N, Miyazawa K, Sugihara M, Hayashi T, Goto D, Ito S, Sumida T.  Six-transmembrane epithelial antigen of prostate4 (STEAP4) is a tumor necrosis factor alpha-induced protein that regulates IL-6, IL-8, and cell proliferation in synovium from patients with rheumatoid arthritis.  Mod Rheumatol  2011  22:128-36  PubMed ID: 21633911
7997  Bader RA, Silvers AL, Zhang N.  Polysialic acid-based micelles for encapsulation of hydrophobic drugs.  Biomacromolecules  2011  12:314-20  PubMed ID: 21218771
8289  Bader RA, Wagoner KL.  Modulation of the response of rheumatoid arthritis synovial fibroblasts to proinflammatory stimulants with cyclic tensile strain.  Cytokine  2010  51:35-41  PubMed ID: 20399680
8449  Nozawa K, Fujishiro M, Kawasaki M, Kaneko H, Iwabuchi K, Yanagida M, Suzuki F, Miyazawa K, Takasaki Y, Ogawa H, Takamori K, Sekigawa I.  Connective tissue growth factor promotes articular damage by increased osteoclastogenesis in patients with rheumatoid arthritis.  Arthritis Res. Ther.  2009  11:R174  PubMed ID: 19922639

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