Cell No. : Cell Name RCB1465 : SHIP^(-) DT40  update : 2024/11/13
Comment			SHIP deficient DT40 cells
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kurosaki, Tomohiro
		Originator	Kurosaki, Tomohiro
		Year of deposit	1998
		Another name	H7-11
		Original cell	DT40
		Animal	_avian < Birds
		Tissue	B cell
		Classification	mutant
		Recombinant	recombinant
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_2G93
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage cell No		1.5-2 x 10 5 cells/ml
		Passage ratio		1 : 20 split
		SC frequency		Subculture : every 2 days
		Temperature		39.5 ℃
		CO2 concentration		5 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Others		Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information		Reference	3
		User's Publication	2

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Reference

2336  Maeda A, Kurosaki M, Ono M, Takai T, Kurosaki T.  Requirement of SH2-containing protein tyrosine phosphatases SHP-1 and SHP-2 for paired immunoglobulin-like receptor B (PIR-B)-mediated inhibitory signal.  J Exp Med  1998  187(8):1355-60  PubMed ID: 9547347   DOI: 10.1084/jem.187.8.1355

2280  Ono 1, Okada H, Bolland S, Yanagi S, Kurosaki T, Ravetch JV.  Deletion of SHIP or SHP-1 reveals two distinct pathways for inhibitory signaling.  Cell  1997  90(2):293-301  PubMed ID: 9244303   DOI: 10.1016/s0092-8674(00)80337-2

2284  Takata M, Sabe H, Hata A, Inazu T, Homma Y, Nukada T, Yamamura H, Kurosaki T.  Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways.  EMBO J  1994  13(6):1341-9  PubMed ID: 8137818   DOI: 10.1002/j.1460-2075.1994.tb06387.x

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User's Publication

8598  Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.  Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.  PLoS Genet.  2009  5:e1000461  PubMed ID: 19390601   DOI: 10.1371/journal.pgen.1000461

16949  Henshall TL, Jones KL, Wilkinson R, Jackson DE.  Src homology 2 domain-containing protein-tyrosine phosphatases, SHP-1 and SHP-2, are required for platelet endothelial cell adhesion molecule-1/CD31-mediated inhibitory signaling  J Immunol  2001  166(5):3098-106  PubMed ID: 11207261   DOI: 10.4049/jimmunol.166.5.3098