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RCB1464 : DT40  update : 2018/07/06
CommentChicken B cell line transformed by avian leukosis virus. c-myc and IgM expressing.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kurosaki, Tomohiro
Originator Humphies, H. Eric.
Year of deposit 1988
Animal avian < Birds
Tissue B cell
Case history avian leukosis virus (ALV)-induced lymphomas
Classification transformed
History Takeda, Syunnichi ( Kyouto Univ.)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus Avian Influenza Virus (-)
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images
deposit info
lot info
Reference information Reference 4
User's Publication 17


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Reference
2740  Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells.  Yamaguchi-Iwai Y, Sonoda E, Sasaki MS, Morrison C, Haraguchi T, Hiraoka Y, Yamashita YM, Yagi T, Takata M, Price C, Kakazu N, Takeda S  EMBO J  1999  18:6619-29  PubMed ID: 10581236
2741  The controlling role of ATM in homologous recombinational repair of DNA damage.  Morrison C, Sonoda E, Takao N, Shinohara A, Yamamoto K, Takeda S  EMBO J  2000  19:463-71  PubMed ID: 10654944
2285  Cell lines derived from avian lymphomas exhibit two distinct phenotypes.  Baba, T W, Giroir, B P, Humphries, E H  Virology  1985  144:139-51  PubMed ID: 2998040
4960  Light chain gene conversion continues at high rate in an ALV-induced cell line.  Buerstedde JM, Reynaud CA, Humphries EH, Olson W, Ewert DL, Weill JC.  EMBO J  1990  9(3):921-7  PubMed ID: 2155784

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User's Publication
2876  Reversible switching of immunoglobulin hypermutation machinery in a chicken B cell line.  Kanayama N, Todo K, Reth M, Ohmori H  Biochem Biophys Res Commun  2005  327:70-5  PubMed ID: 15629431
3086  Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair.  Yin, Hong Lan, Suzuki, Yuka, Matsumoto, Yoshihisa, Tomita, Masanori, Furusawa, Yoshiya, Enomoto, Atsushi, Morita, Akinori, Aoki, Mizuho, Yatagai, Fumio, Suzuki, Takahiko, Hosoi, Yoshio, Ohtomo, Kuni, Suzuki, Norio  Radiat Res  2004  162:433-41  PubMed ID: 15447039
3371  The role of canonical transient receptor potential 7 in B-cell receptor-activated channels.  Lievremont, Jean-Philippe, Numaga, Takuro, Vazquez, Guillermo, Lemonnier, Loic, Hara, Yuji, Mori, Emiko, Trebak, Mohamed, Moss, Stephen E, Bird, Gary S, Mori, Yasuo, Putney, James W Jr  J Biol Chem  2005  280:35346-51  PubMed ID: 16123040
7115  Rac1/p21-activated kinase pathway controls retinoblastoma protein phosphorylation and E2F transcription factor activation in B lymphocytes.  Zaldua N, Llavero F, Artaso A, Galvez P, Lacerda HM, Parada LA, Zugaza JL.  FEBS J.  2015  283:647-61  PubMed ID: 26663827
8442  Lysine acetylation regulates Bruton's tyrosine kinase in B cell activation.  Liu Z, Mai A, Sun J.  J. Immunol.  2009  184:244-54  PubMed ID: 19949111
8598  Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.  Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.  PLoS Genet.  2009  5:e1000461  PubMed ID: 19390601
8809  A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics.  Honda H, Ichiyanagi K, Suzuki J, Ono T, Koyama H, Kajikawa M, Okada N.  Gene  2007  395:116-24  PubMed ID: 17434692
9254  In vivo transfer of a neuronal nitric oxide synthase expression vector into the rat bladder by electroporation.  Iwashita H, Yoshida M, Nishi T, Otani M, Ueda S.  BJU Int.  2004  93:1098-103  PubMed ID: 15142171
9376  A calmodulin/inositol 1,4,5-trisphosphate (IP3) receptor-binding region targets TRPC3 to the plasma membrane in a calmodulin/IP3 receptor-independent process.  Wedel BJ, Vazquez G, McKay RR, St J Bird G, Putney JW Jr.  J. Biol. Chem.  2003  278:25758-65  PubMed ID: 12730194
9379  Expression level of the canonical transient receptor potential 3 (TRPC3) channel determines its mechanism of activation.  Vazquez G, Wedel BJ, Trebak M, St John Bird G, Putney JW Jr.  J. Biol. Chem.  2003  278:21649-54  PubMed ID: 12686562
9416  An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes.  Vazquez G, Wedel BJ, Bird GS, Joseph SK, Putney JW.  EMBO J.  2002  21:4531-8  PubMed ID: 12198155
9434  Comparison of human TRPC3 channels in receptor-activated and store-operated modes. Differential sensitivity to channel blockers suggests fundamental differences in channel composition.  Trebak M, Bird GS, McKay RR, Putney JW Jr.  J. Biol. Chem.  2002  277:21617-23  PubMed ID: 11943785
9450  Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes.  Vazquez G, Lievremont JP, St J Bird G, Putney JW Jr.  Proc. Natl. Acad. Sci. U.S.A.  2001  98:11777-82  PubMed ID: 11553786
9452  Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry.  Broad LM, Braun FJ, Lievremont JP, Bird GS, Kurosaki T, Putney JW Jr.  J. Biol. Chem.  2001  276:15945-52  PubMed ID: 11278938
10136  SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus.  Kawaguchi Y, Nariki H, Kawamoto N, Kanehiro Y, Miyazaki S, Suzuki M, Magari M, Tokumitsu H, Kanayama N.  Biochem. Biophys. Res. Commun.  2017  485:261-266  PubMed ID: 28235482
10282  Regulation of axon arborization pattern in the developing chick ciliary ganglion: Possible involvement of caspase 3.  Katow H, Kanaya T, Ogawa T, Egawa R, Yawo H.  Dev. Growth Differ.  2017  59:115-128  PubMed ID: 28430358
4398  NVP-LAQ824 is a potent novel histone deacetylase inhibitor with significant activity against multiple myeloma.  Catley L, Weisberg E, Tai YT, Atadja P, Remiszewski S, Hideshima T, Mitsiades N, Shringarpure R, LeBlanc R, Chauhan D, Munshi NC, Schlossman R, Richardson P, Griffin J, Anderson KC.  Blood  2003  102(7):2615-22  PubMed ID: 12816865



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