Cell No. : Cell Name
RCB1435 : KE-97
update : 2024/01/17
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| Comment | Stopped distribution. Until 2022, this cell line has been distributed as “Human cell line derived from intraperitoneal metastasis of gastric cancer”. However, this cell line was proved to be derived from B cells, and thus we stopped the distribution. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. Commercial use is prohibited by the DEPOSITOR. |
| Remarks | |
| Order Form |
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Uesugi, Hidenaga
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| Originator |
Yamashita, Kazuya
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| Year of deposit |
1998
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Male
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| Age at sampling |
52 years
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| Tissue |
stomach, mesenterium meta
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| Classification |
cancer
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| Year of origin |
1989
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Lifespan |
infinite
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| Morphology |
other
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| Cellosaurus(Expasy) |
CVCL_3386
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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pipetting
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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46-48(50) : 46(3),47(41),48(6)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
11
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| User's Publication |
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16129
Nagamura Y, Miyazaki M, Nagano Y, Tomiyama A, Ohki R, Yanagihara K, Sakai R, Yamaguchi H.
SHP2 as a Potential Therapeutic Target in Diffuse-Type Gastric Carcinoma Addicted to Receptor Tyrosine Kinase Signaling
Cancers (Basel)
2021
13(17):4309
PubMed ID: 34503119
DOI: 10.3390/cancers13174309
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11505
Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.
Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.
Biochem Biophys Rep
2019
18:100632
PubMed ID: 30993217
DOI: 10.1016/j.bbrep.2019.100632
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10662
Miyamoto S, Nagamura Y, Nakabo A, Okabe A, Yanagihara K, Fukami K, Sakai R, Yamaguchi H.
Aberrant alternative splicing of RHOA is associated with loss of its expression and activity in diffuse-type gastric carcinoma cells.
Biochem. Biophys. Res. Commun.
2018
495:1942-1947
PubMed ID: 29247652
DOI: 10.1016/j.bbrc.2017.12.067
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10586
Ishii S, Yamashita K, Harada H, Ushiku H, Tanaka T, Nishizawa N, Yokoi K, Washio M, Ema A, Mieno H, Moriya H, Hosoda K, Waraya M, Katoh H, Watanabe M.
The H19-PEG10/IGF2BP3 axis promotes gastric cancer progression in patients with high lymph node ratios.
Oncotarget
2017
8:74567-74581
PubMed ID: 29088808
DOI: 10.18632/oncotarget.20209
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5812
Hirashita Y et al
Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.
Cancer Sci
2016
107(12):1919-1928
PubMed ID: 27699948
DOI: 10.1111/cas.13094
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12639
Yamashita K, Kuno A, Matsuda A, Ikehata Y, Katada N, Hirabayashi J, Narimatsu H, Watanabe M.
Lectin microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer
Gastric Cancer
2016
19(2):531-54
PubMed ID: 25840959
DOI: 10.1007/s10120-015-0491-2
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5837
Liu J et al
Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat Commun
2014
5:3830
PubMed ID: 24807215
DOI: 10.1038/ncomms4830
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5302
Yamaguchi H, Takanashi M, Yoshida N, Ito Y, Kamata R, Fukami K, Yanagihara K, Sakai R.
Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors.
Cancer Sci
2014
105(5):528-36
PubMed ID: 24612061
DOI: 10.1111/cas.12387
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5845
Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.
Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.
PLoS One
2013
8(2):e56766
PubMed ID: 23451082
DOI: 10.1371/journal.pone.0056766
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15081
Awazu Y, Nakamura K, Mizutani A, Kakoi Y, Iwata H, Yamasaki S, Miyamoto N, Imamura S, Miki H, Hori A.
A novel inhibitor of c-Met and VEGF receptor tyrosine kinases with a broad spectrum of in vivo antitumor activities
Mol Cancer Ther
2013
12(6):913-24
PubMed ID: 23548264
DOI: 10.1158/1535-7163.MCT-12-1011
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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