Cell No. : Cell Name RCB1376 : 2-7  update : 2023/09/27
Comment			CITES. A copy of CAG-loxP-neopA-loxP-LacZ was inserted. Cre will delete neopA and induce LacZ. Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES). Origin animal species details
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Saito, Izumi
		Originator	Kanegae, Yumi
		Year of deposit	1997
		Animal	_monkey < Mammals
		Strain name	African green Origin animal species details
		Genus	Cercopithecus
		Species	aethiops
		Tissue	kidney
		Classification	transformed
		Recombinant	recombinant
		Exogene	pCAGGS
		History	originator -> Saito, Izumi
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_T366
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS + 1mg/ml G418
		Antibiotics		Free
		Passage method		(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	1
		User's Publication	1

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Reference

2217  Kanegae Y, Takamori K, Sato Y, Lee G, Nakai M, Saito I  Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase.  Gene  1996  181:207-12  PubMed ID: 8973332   DOI: 10.1016/s0378-1119(96)00516-1

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User's Publication

16156  Baba Y, Nakano M, Yamada Y, Saito I, Kanegae Y.  Practical range of effective dose for Cre recombinase-expressing recombinant adenovirus without cell toxicity in mammalian cells  Microbiol Immunol  2005  49(6):559-70  PubMed ID: 15965304   DOI: 10.1111/j.1348-0421.2005.tb03753.x