Cell No. : Cell Name
RCB1370 : NBT-T2
update : 2025/07/25
|
| Comment | Stopped distribution temporarily.A subline of NBT-II. Almost non-invasive bladder carcinoma. Relatively high cadherin expression. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishi, Nozomu
|
| Originator |
Nishi, Nozomu
|
| Year of deposit |
1997
|
| Animal |
_rat
< Mammals
|
| Strain name |
Wistar
|
| Genus |
Rattus
|
| Species |
norvegicus
|
| Gender |
Male
|
| Tissue |
bladder
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_4675
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, AST, MPI, PepB
|
| Reference information |
Reference |
2
|
| User's Publication |
9
|
| User's Publication |
|
22610
Kobayashi S, Kashiwagi H, Kobayashi K, Kohzaki M.
4,4'-methylenebis(2-chloroaniline) induces chromosome aneuploidy associated with premature chromatid separation in mammalian cells: A possible carcinogenic mechanism.
Ecotoxicol Environ Saf
2025
293:117981
PubMed ID: 40073788
DOI: 10.1016/j.ecoenv.2025.117981
|
|
13594
Hanif N, Murni A, Tanaka J.
Sangiangols A and B, Two New Dolabellanes from an Indonesian Marine Soft Coral, Anthelia sp
Molecules
2020
25(17):3803
PubMed ID: 32825594
DOI: 10.3390/molecules25173803
|
|
19455
Trianto A, Hermawan I, Suzuka T, Tanaka J.
Two new cytotoxic candidaspongiolides from an indonesian sponge
ISRN Pharm
2011
2011:852619
PubMed ID: 22389864
DOI: 10.5402/2011/852619
|
|
19456
Uddin MH, Hanif N, Trianto A, Agarie Y, Higa T, Tanaka J.
Four new polyoxygenated gorgosterols from the gorgonian Isis hippuris
Nat Prod Res
2011
25(6):585-91
PubMed ID: 21409719
DOI: 10.1080/14786419.2010.485303
|
|
19457
Hermawan I, de Voogd NJ, Tanaka J.
An acetylenic alkaloid from the calcareous sponge Leucetta sp
Mar Drugs
2011
9(3):382-6
PubMed ID: 21556167
DOI: 10.3390/md9030382
|
|
19458
Mohammad Helal Uddin, Michael Chandro Roy, Junichi Tanaka
Cytotoxic cholic acid type sterones from a marine soft coral Paraminabea sp.
Chemistry of Natural Compounds
2011
47:64-67
DOI: 10.1007/s10600-011-9831-6
|
|
16166
Trianto A, Hermawan I, de Voogd NJ, Tanaka J.
Halioxepine, a new meroditerpene from an Indonesian sponge Haliclona sp
Chem Pharm Bull (Tokyo)
2011
59(10):1311-3
PubMed ID: 21963646
DOI: 10.1248/cpb.59.1311
|
|
19337
Aratake S, Trianto A, Hanif N, de Voogd NJ, Tanaka J.
A new polyunsaturated brominated fatty acid from a Haliclona sponge
Mar Drugs
2009
7(4):523-7
PubMed ID: 20098595
DOI: 10.3390/md7040523
|
|
16072
Nagasaki A, Inotsume K, Kanada M, Uyeda TQ.
Phospholipase D is essential for keratocyte-like migration of NBT-II cells
Cell Struct Funct
2008
33(1):27-33
PubMed ID: 18344600
DOI: 10.1247/csf.07042
|
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