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Cell No. : Cell Name
RCB1367 : HuH-6  update : 2024/10/04
CommentHepatoblastoma. Said albumin and alpha-fetoprotein producing.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Dr.SAKAGUCHI Masakiyo
Fax. +81-86-235-7400
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Huh, Nam, Ho
Originator Doi, Isao
Year of deposit 1997
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 1 year
Tissue liver
Classification cancer
Year of origin 1974
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4381
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 80-92(50) : 80(4),81(4),82(7),83(6),84(3),85(4)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 42


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Reference
3427  H Nakabayashi, K Taketa, T Yamane, M Oda, J Sato  Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines p  Cancer Res  1985  45(12 Pt 1):6379-83  PubMed ID: 2415243  
2578  Nakabayashi H, Taketa K, Yamane T, Miyazaki M, Miyano K, Sato J  Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.  Gann  1984  75:151-8  PubMed ID: 6203805  
135  Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J  Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.  Cancer Res  1982  42:3858-63  PubMed ID: 6286115  
140  Doi I  Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.  Gann  1976  67:1-10  PubMed ID: 57894  

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User's Publication
21541  Li F, Liu P, Mi W, Li L, Anderson NM, Lesner NP, Burrows M, Plesset J, Majer A, Wang G, Li J, Zhu L, Keith B, Simon MC.  Blocking methionine catabolism induces senescence and confers vulnerability to GSK3 inhibition in liver cancer.  Nat Cancer  2024    PubMed ID: 38168934   DOI: 10.1038/s43018-023-00671-3
22304  Lee SCS, Pyo AHA, Mohammadi H, Zhang J, Dvorkin-Gheva A, Malbeteau L, Chung S, Khan S, Ciudad MT, Rondeau V, Cairns RA, Kislinger T, McGaha TL, Wouters BG, Reisz JA, Culp-Hill R, D'Alessandro A, Jones CL, Koritzinsky M.  Cysteamine dioxygenase (ADO) governs cancer cell mitochondrial redox homeostasis through proline metabolism  Sci Adv  2024  10(40):eadq0355  PubMed ID: 39356760   DOI: 10.1126/sciadv.adq0355
20598  Sakaguchi H, Tsuchiya H, Kitagawa Y, Tanino T, Yoshida K, Uchida N, Shiota G.  NEAT1 Confers Radioresistance to Hepatocellular Carcinoma Cells by Inducing Autophagy through GABARAP.  Int J Mol Sci  2022  23(2)  PubMed ID: 35054896   DOI: 10.3390/ijms23020711
12538  Kaneya Y, Takata H, Wada R, Kure S, Ishino K, Kudo M, Kondo R, Taniai N, Ohashi R, Yoshida H, Naito Z.  Inhibitor for protein disulfide-isomerase family A member 3 enhances the antiproliferative effect of inhibitor for mechanistic target of rapamycin in liver cancer: An in vitro study on combination treatment with everolimus and 16F16  Oncol Lett  2021  21(1):28  PubMed ID: 33240434   DOI: 10.3892/ol.2020.12289
20042  Woodfield SE, Shi Y, Patel RH, Chen Z, Shah AP, Whitlock RS, Ibarra AM, Larson SR, Sarabia SF, Badachhape A, Starosolski Z, Ghaghada KB, Sumazin P, Annis DA, López-Terrada D, Vasudevan SA.  MDM4 inhibition: a novel therapeutic strategy to reactivate p53 in hepatoblastoma.  Sci Rep  2021  11(1):2967  PubMed ID: 33536467   DOI: 10.1038/s41598-021-82542-4
18208  Qianyu Zhang, Liqun Wang, Cailing Gan, Yan Yu, Yali Li, Yuanle Deng, Hongyao Liu, Jia You, Wenya Yin  Cryptotanshinone Induces Apoptosis and Inhibits Migration and Invasion in Human Hepatocellular Carcinoma Cells In Vitro  Natural Product Communications  2020  15:1934578X1989957    DOI: 10.1177/1934578x19899570
13053  Kim DH, Kim WD, Kim SK, Moon DH, Lee SJ.  TGF-β1-mediated repression of SLC7A11 drives vulnerability to GPX4 inhibition in hepatocellular carcinoma cells.  Cell Death Dis  2020    PubMed ID: 32471991   DOI: 10.1038/s41419-020-2618-6
13067  Zheng J, Guo X, Shioya A, Yoshioka T, Matsumoto K, Hiraki T, Kusano H, Oyama T, Kurose N, Yamaguchi R, Uramoto H, Ieiri S, Okajima H, Kohno M, Yamada S.  Peroxiredoxin 4 promotes embryonal hepatoblastoma cell migration but induces fetal cell differentiation.  Am J Transl Res  2020    PubMed ID: 32655804  
13103  Sekiguchi M, Seki M, Kawai T, Yoshida K, Yoshida M, Isobe T, Hoshino N, Shirai R, Tanaka M, Souzaki R, Watanabe K, Arakawa Y, Nannya Y, Suzuki H, Fujii Y, Kataoka K, Shiraishi Y, Chiba K, Tanaka H, Shimamura T, Sato Y, Sato-Otsubo A, Kimura S, Kubota Y, Hiwatari M, Koh K, Hayashi Y, Kanamori Y, Kasahara M, Kohashi K, Kato M, Yoshioka T, Matsumoto K, Oka A, Taguchi T, Sanada M, Tanaka Y, Miyano S, Hata K, Ogawa S, Takita J.  Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets.  NPJ Precis Oncol  2020    PubMed ID: 32656360   DOI: 10.1038/s41698-020-0125-y
13191  Xu DQ, Yuan XJ, Hirayama M, Toyoda H.  Huaier Extract Induces Apoptosis in Hepatoblastoma Cells Via the MEK/ERK Signaling Pathway.  In Vivo  2020    PubMed ID: 32871763   DOI: 10.21873/invivo.12051
11250  Jin J, Jin J, Woodfield SE, Patel RH, Jin NG, Shi Y, Liu B, Sun W, Chen X, Yu Y, Vasudevan SA.  Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation.  Oncol. Rep.  2019    PubMed ID: 30320362   DOI: 10.3892/or.2018.6793
9813  Dina Kats, Cora A Ricker, Noah E Berlow, Bénédicte Noblet, Delphine Nicolle, Katell Mevel, Sophie Branchereau, Jean-Gabriel Judde, Cody D Stiverson, Christina L Stiverson, Matthew N Svalina, Teagan Settelmeyer, Kevin Matlock, Melvin Lathara, Charlotte Mussini, James I Geller, Christopher Noakes, Ido Sloma, Narendra Bharathy, Stefano Cairo, Charles Keller  Volasertib Preclinical Activity in High-Risk Hepatoblastoma  Oncotarget   2019  10(60):6403-6417  PubMed ID: 31741706   DOI: 10.18632/oncotarget.27237
10671  Woodfield SE, Shi Y, Patel RH, Jin J, Major A, Sarabia SF, Starosolski Z, Zorman B, Gupta SS, Chen Z, Ibarra AM, Bissig KD, Ghaghada KB, Sumazin P, López-Terrada D, Vasudevan SA.  A Novel Cell Line Based Orthotopic Xenograft Mouse Model That Recapitulates Human Hepatoblastoma.  Sci Rep  2017  7:17751  PubMed ID: 29259231   DOI: 10.1038/s41598-017-17665-8
14715  Goto H, Kitagawa N, Sekiguchi H, Miyagi Y, Keino D, Sugiyama M, Sarashina T, Miyagawa N, Yokosuka T, Hamanoue S, Iwasaki F, Shiomi M, Goto S, Tanaka Y.  The Collagen Gel Droplet-embedded Culture Drug Sensitivity Test in Relapsed Hepatoblastoma  J Pediatr Hematol Oncol  2017  39(5):395-401  PubMed ID: 28562516   DOI: 10.1097/MPH.0000000000000865
19032  Dauch D, Rudalska R, Cossa G, Nault JC, Kang TW, Wuestefeld T, Hohmeyer A, Imbeaud S, Yevsa T, Hoenicke L, Pantsar T, Bozko P, Malek NP, Longerich T, Laufer S, Poso A, Zucman-Rossi J, Eilers M, Zender L.  A MYC-aurora kinase A protein complex represents an actionable drug target in p53-altered liver cancer  Nat Med  2016  22(7):744-53  PubMed ID: 27213815   DOI: 10.1038/nm.4107
12915  Bissig-Choisat B, Kettlun-Leyton C, Legras XD, Zorman B, Barzi M, Chen LL, Amin MD, Huang YH, Pautler RG, Hampton OA, Prakash MM, Yang D, Borowiak M, Muzny D, Doddapaneni HV, Hu J, Shi Y, Gaber MW, Hicks MJ, Thompson PA, Lu Y, Mills GB, Finegold M, Goss JA, Parsons DW, Vasudevan SA, Sumazin P, López-Terrada D, Bissig KD.  Novel patient-derived xenograft and cell line models for therapeutic testing of pediatric liver cancer  J Hepatol  2016  65(2):325-33  PubMed ID: 27117591   DOI: 10.1016/j.jhep.2016.04.009
7481  Saida S, Watanabe K, Kato I, Fujino H, Umeda K, Okamoto S, Uemoto S, Hishiki T, Yoshida H, Tanaka S, Adachi S, Niwa A, Nakahata T, Heike T.  Prognostic significance of aminopeptidase-N (CD13) in hepatoblastoma.  Pediatr Int  2015  57:558-66  PubMed ID: 25682862   DOI: 10.1111/ped.12597
14012  Gao L, Ge C, Fang T, Zhao F, Chen T, Yao M, Li J, Li H.  ANGPTL2 promotes tumor metastasis in hepatocellular carcinoma  J Gastroenterol Hepatol  2015  30(2):396-404  PubMed ID: 25090954   DOI: 10.1111/jgh.12702
15187  Evason KJ, Francisco MT, Juric V, Balakrishnan S, Lopez Pazmino Mdel P, Gordan JD, Kakar S, Spitsbergen J, Goga A, Stainier DY.  Identification of Chemical Inhibitors of β-Catenin-Driven Liver Tumorigenesis in Zebrafish  PLoS Genet  2015  11(7):e1005305  PubMed ID: 26134322   DOI: 10.1371/journal.pgen.1005305
18954  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Sueishi M.  Short hairpin RNA of frizzled-2 suppresses the proliferation of hepatocellular carcinoma cells  Oncol Lett  2014  8(4):1519-1522  PubMed ID: 25202360   DOI: 10.3892/ol.2014.2408
9195  Luca Quagliata , Matthias S Matter, Salvatore Piscuoglio, Leila Arabi, Christian Ruiz, Alfredo Procino, Michal Kovac, Francesca Moretti, Zuzanna Makowska, Tujana Boldanova, Jesper B Andersen, Monika Hämmerle, Luigi Tornillo, Markus H Heim, Sven Diederichs, Clemente Cillo, Luigi M Terracciano  Long Noncoding RNA HOTTIP/HOXA13 Expression Is Associated With Disease Progression and Predicts Outcome in Hepatocellular Carcinoma Patients  Hepatology  2014  59 (3), 911-23  PubMed ID: 24114970   DOI: 10.1002/hep.26740
18958  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Niclosamide suppresses Hepatoma cell proliferation via the Wnt pathway  Onco Targets Ther  2013  6:1685-93  PubMed ID: 24273411   DOI: 10.2147/OTT.S50065
7757  Lin WH, Martin JL, Marsh DJ, Jack MM, Baxter RC.  Involvement of insulin-like growth factor-binding protein-3 in the effects of histone deacetylase inhibitor MS-275 in hepatoma cells.  J. Biol. Chem.  2011  286:29540-7  PubMed ID: 21737444   DOI: 10.1074/jbc.M111.263111
6009  Di Masi A, Viganotti M, Antoccia A, Magrelli A, Salvatore M, Azzalin G, Tosto F, Lorenzetti S, Maranghi F, Mantovani A, Macino G, Tanzarella C, Taruscio D.  Characterization of HuH6, Hep3B, HepG2 and HLE liver cancer cell lines by WNT/β - catenin pathway, microRNA expression and protein expression profile.  Cell Mol Biol (Noisy-le-grand)  2010  56 Suppl:OL1299-317  PubMed ID: 20937217  
16969  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Sorafenib suppresses the cell cycle and induces the apoptosis of hepatocellular carcinoma cell lines in serum-free media  Exp Ther Med  2010  1(5):863-866  PubMed ID: 22993610   DOI: 10.3892/etm.2010.131
16903  Fujimoto T, Tomizawa M, Yokosuka O.  SiRNA of frizzled-9 suppresses proliferation and motility of hepatoma cells  Int J Oncol  2009  35(4):861-6.  PubMed ID: 19724923   DOI: 10.3892/ijo_00000400
18303  Dharel N, Kato N, Muroyama R, Taniguchi H, Otsuka M, Wang Y, Jazag A, Shao RX, Chang JH, Adler MK, Kawabe T, Omata M.  Potential contribution of tumor suppressor p53 in the host defense against hepatitis C virus  Hepatology   2008  47(4):1136-49  PubMed ID: 18220274   DOI: 10.1002/hep.22176
18311  Mitsuoka K, Miyoshi S, Kato Y, Murakami Y, Utsumi R, Kubo Y, Noda A, Nakamura Y, Nishimura S, Tsuji A.  Cancer detection using a PET tracer, 11C-glycylsarcosine, targeted to H+/peptide transporter  J Nucl Med  2008  49(4):615-22  PubMed ID: 18344442   DOI: 10.2967/jnumed.107.048231
18314  Tanaka Y, Kanai F, Tada M, Tateishi R, Sanada M, Nannya Y, Ohta M, Asaoka Y, Seto M, Shiina S, Yoshida H, Kawabe T, Yokosuka O, Ogawa S, Omata M.  Gain of GRHL2 is associated with early recurrence of hepatocellular carcinoma  J Hepatol  2008  49(5):746-57.  PubMed ID: 18752864   DOI: 10.1016/j.jhep.2008.06.019
18678  Kadenhe-Chiweshe A, Papa J, McCrudden KW, Frischer J, Bae JO, Huang J, Fisher J, Lefkowitch JH, Feirt N, Rudge J, Holash J, Yancopoulos GD, Kandel JJ, Yamashiro DJ.  Sustained VEGF blockade results in microenvironmental sequestration of VEGF by tumors and persistent VEGF receptor-2 activation  Mol Cancer Res  2008  6(1):1-9  PubMed ID: 18234958   DOI: 10.1158/1541-7786.MCR-07-0101
18987  Tomizawa M, Saisho H.  Insulin-like growth factor (IGF)-II regulates CCAAT/enhancer binding protein alpha expression via phosphatidyl-inositol 3 kinase in human hepatoblastoma cell lines  J Cell Biochem  2007  102(1):161-70  PubMed ID: 17372916   DOI: 10.1002/jcb.21293
3077  Nakatsu N, Yoshida Y, Yamazaki K, Nakamura T, Dan S, Fukui Y, Yamori T  Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.  Mol Cancer Ther  2005  4:399-412  PubMed ID: 15767549   DOI: 10.1158/1535-7163.MCT-04-0234
3471  Hayakawa K, Nagamine T, Li X-K, Katsumata N, Ogata T, Tanaka T  Affinity chromatographic determination of D-aspartic in the liver cell lines.  Trends in Chromatography  2005  1:105-110   
3182  Kou, Ikuyo, Ikegawa, Shiro  SOX9-dependent and -independent transcriptional regulation of human cartilage link protein.  J Biol Chem  2004  279:50942-8  PubMed ID: 15456769   DOI: 10.1074/jbc.M406786200
2660  Hayakawa, Kou, Guo, Lei, Terentyeva, Elena A, Li, Xiao Kang, Kimura, Hiromitsu, Hirano, Masahiko, Yoshikawa, Kazuyuki, Yoshinaga, Teruo, Nagamine, Takeaki, Katsumata, Noriyuki, Tanaka, Toshiaki  Size-exclusion chromatography of biological samples which contain extremely alkaline proteins.  J Biochem Biophys Methods  2003  56:153-63  PubMed ID: 12834974   DOI: 10.1016/s0165-022x(03)00056-3
17310  McCrudden KW, Hopkins B, Frischer J, Novikov A, Huang J, Kadenhe A, New T, Yokoi A, Yamashiro DJ, Kandel JJ, Middlesworth W.  Anti-VEGF antibody in experimental hepatoblastoma: suppression of tumor growth and altered angiogenesis  J Pediatr Surg  2003  38(3)  PubMed ID: 12632340   DOI: 10.1053/jpsu.2003.50099
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014   DOI: 10.1111/j.1349-7006.2002.tb02174.x
1307  Jun You, Masamichi Kamihira, Shinji Iijima  Enhancement of transfection efficiency using ligand-modified lipid vesicles  Journal of Fermentation and Bioengineering  1998  85:525-528    DOI: 10.1016/s0922-338x(98)80075-5
2115  Kosaka T, Tsuboi S, Fukaya K, Pu H, Ohno T, Tsuji T, Miyazaki M, Namba M  Spheroid cultures of human hepatoblastoma cells (HuH-6 line) and their application for cytotoxicity assay of alcohols.  Acta Med Okayama  1996  50:61-6  PubMed ID: 8744930   DOI: 10.18926/AMO/30490
2123  Kosaka T, Fukaya K, Tsuboi S, Pu H, Ohno T, Tsuji T, Namba M  Comparison of various methods of assaying the cytotoxic effects of ethanol on human hepatoblastoma cells (HUH-6 line).  Acta Med Okayama  1996  50:151-6  PubMed ID: 8805855   DOI: 10.18926/AMO/30502
1859  Gu.,Y., Tokiwa T., Kino K., Ohno T. and Namba M.  Cytotoxicity Test of Methanol Using Various Human Hepatoma Cell Lines  Alternatives to Animal Testing and Experimentation(AATEX)  1993  2:105-108   
1744  Gu.,Y., Miyazaki, M., Ohno, T. and Namba M.  Cytotoxicity Test of Ethanol Using Various Human Hepatoma and Other Cell Lines  Alternatives to Animal Testing and Experimentation(AATEX)  1993  2:1-5   


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