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RCB1367 : HuH-6  update : 2018/07/06
CommentHepatoblastoma. Said albumin and alpha-fetoprotein producing.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Dr.Sakaguchi, Masakiyo
Fax. +81-86-235-7400
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Huh, Nam, Ho
Originator Doi, Isao
Year of deposit 1997
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 1 year
Tissue liver
Classification cancer
Year of origin 1974
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 80-92(50) : 80(4),81(4),82(7),83(6),84(3),85(4)/
Images
deposit info
lot info
Reference information Reference 3
User's Publication 11


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Reference
140  Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.  Doi I  Gann  1976  67:1-10  PubMed ID: 57894
2578  Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.  Nakabayashi H, Taketa K, Yamane T, Miyazaki M, Miyano K, Sato J  Gann  1984  75:151-8  PubMed ID: 6203805
135  Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.  Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J  Cancer Res  1982  42:3858-63  PubMed ID: 6286115

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User's Publication
3077  Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.  Nakatsu N, Yoshida Y, Yamazaki K, Nakamura T, Dan S, Fukui Y, Yamori T  Mol Cancer Ther  2005  4:399-412  PubMed ID: 15767549
3471  Affinity chromatographic determination of D-aspartic in the liver cell lines.  Hayakawa K, Nagamine T, Li X-K, Katsumata N, Ogata T, Tanaka T  Trends in Chromatography  2005  1:105-110 
2660  Size-exclusion chromatography of biological samples which contain extremely alkaline proteins.  Hayakawa, Kou, Guo, Lei, Terentyeva, Elena A, Li, Xiao Kang, Kimura, Hiromitsu, Hirano, Masahiko, Yoshikawa, Kazuyuki, Yoshinaga, Teruo, Nagamine, Takeaki, Katsumata, Noriyuki, Tanaka, Toshiaki  J Biochem Biophys Methods  2003  56:153-63  PubMed ID: 12834974
3182  SOX9-dependent and -independent transcriptional regulation of human cartilage link protein.  Kou, Ikuyo, Ikegawa, Shiro  J Biol Chem  2004  279:50942-8  PubMed ID: 15456769
3342  The combination of SOX5, SOX6, and SOX9 (the SOX trio) provides signals sufficient for induction of permanent cartilage.  Ikeda, Toshiyuki, Kamekura, Satoru, Mabuchi, Akihiko, Kou, Ikuyo, Seki, Shoji, Takato, Tsuyoshi, Nakamura, Kozo, Kawaguchi, Hiroshi, Ikegawa, Shiro, Chung, Ung-il  Arthritis Rheum  2004  50:3561-73  PubMed ID: 15529345
7481  Prognostic significance of aminopeptidase-N (CD13) in hepatoblastoma.  Saida S, Watanabe K, Kato I, Fujino H, Umeda K, Okamoto S, Uemoto S, Hishiki T, Yoshida H, Tanaka S, Adachi S, Niwa A, Nakahata T, Heike T.  Pediatr Int  2015  57:558-66  PubMed ID: 25682862
7757  Involvement of insulin-like growth factor-binding protein-3 in the effects of histone deacetylase inhibitor MS-275 in hepatoma cells.  Lin WH, Martin JL, Marsh DJ, Jack MM, Baxter RC.  J. Biol. Chem.  2011  286:29540-7  PubMed ID: 21737444
10671  A Novel Cell Line Based Orthotopic Xenograft Mouse Model That Recapitulates Human Hepatoblastoma.  Woodfield SE, Shi Y, Patel RH, Jin J, Major A, Sarabia SF, Starosolski Z, Zorman B, Gupta SS, Chen Z, Ibarra AM, Bissig KD, Ghaghada KB, Sumazin P, López-Terrada D, Vasudevan SA.  Sci Rep  2017  7:17751  PubMed ID: 29259231
11250  Targeting LRH‑1 in hepatoblastoma cell lines causes decreased proliferation.  Jin J, Jin J, Woodfield SE, Patel RH, Jin NG, Shi Y, Liu B, Sun W, Chen X, Yu Y, Vasudevan SA.  Oncol. Rep.  2019    PubMed ID: 30320362
6009  Characterization of HuH6, Hep3B, HepG2 and HLE liver cancer cell lines by WNT/β - catenin pathway, microRNA expression and protein expression profile.  Di Masi A, Viganotti M, Antoccia A, Magrelli A, Salvatore M, Azzalin G, Tosto F, Lorenzetti S, Maranghi F, Mantovani A, Macino G, Tanzarella C, Taruscio D.  Cell Mol Biol (Noisy-le-grand)  2010  56 Suppl:OL1299-317  PubMed ID: 20937217
2758  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014



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