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細胞番号 : 細胞名
RCB1313 : RERF-LC-KJ  update : 2022/07/28
細胞特性(Comment:英)Japanese lung adenocarcinoma, highly metastatic in SCID mice. Also refer to RCB0444 RCRF-LC-AI.
細胞特性(日)日本人肺腺癌。SCIDマウスに移植後、高頻度に転移を起こす。RCRF-LC-AI(扁平上皮癌)と間違えないように。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Kyoizumi, Seishi
樹立者 Terada, Seigo
寄託日 1997
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Male
78 years
採取組織 lung
病名 adenocarcinoma
転移 Yes
細胞分類 cancer
初代培養日 1992
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_1654
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/7-10 days, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
染色体検査 59-70(50) : /64(7),65(5),66(6),67(6),68(8),69(8)/
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 35件
利用者成果(日) 0件

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Reference(英)
2209  Teraoka S, Kyoizumi S, Seyama T, Yamakido M, Akiyama M  A Novel SCID Mouse Model for Studying Spontaneous Metastasis of Human Lung Cancer to Human Tissue  Jpn J Cancer Res  1995  86:419-423  PubMed ID: 7790314   DOI: 10.1111/j.1349-7006.1995.tb03073.x
2208  Teraoka, S., Kyoizumi, S., Seyama, T., Yamakido, M., and Akiyama, M.  SCID mice model for the in vivo study of human oncothetapy: Studies on the growth and metastasis of human lung cancer  Int J Oncol  1994  5:501-508  PubMed ID: 21559605  

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Reference(日)

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利用者成果(英)
20595  Matsushige T, Sakabe T, Umekita Y.  Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line.  Yonago Acta Med  2022  65(1):44-52  PubMed ID: 35221759   DOI: 10.33160/yam.2022.02.006
13332  Hase H, Aoki M, Matsumoto K, Nakai S, Nagata T, Takeda A, Ueda K, Minami K, Kitae K, Jingushi K, Ueda Y, Yamamoto M, Furukawa T, Sato M, Tsujikawa K.  Cancer type‑SLCO1B3 promotes epithelial‑mesenchymal transition resulting in the tumour progression of non‑small cell lung cancer.  Oncol Rep  2021    PubMed ID: 33155667   DOI: 10.3892/or.2020.7839
20304  Nakasone S, Suzuki A, Okazaki H, Onodera K, Zenkoh J, Ishii G, Suzuki Y, Tsuboi M, Tsuchihara K.  Predictive markers based on transcriptome modules for vinorelbine-based adjuvant chemotherapy for lung adenocarcinoma patients.  Lung Cancer  2021  158:115-125  PubMed ID: 34157583   DOI: 10.1016/j.lungcan.2021.06.011
13773  Saigusa D, Motoike IN, Saito S, Zorzi M, Aoki Y, Kitamura H, Suzuki M, Katsuoka F, Ishii H, Kinoshita K, Motohashi H, Yamamoto M.  Impacts of NRF2 activation in non-small-cell lung cancer cell lines on extracellular metabolites  Cancer Sci  2020  111(2):667-678  PubMed ID: 31828882   DOI: 10.1111/cas.14278
11336  Kosibaty Z, Murata Y, Minami Y, Dai T, Kano J, Matsuoka R, Nakano N, Noguchi M.  Cytoplasmic expression of epithelial cell transforming sequence 2 in lung adenocarcinoma and its implications for malignant progression.  Lab. Invest.  2019    PubMed ID: 30542068   DOI: 10.1038/s41374-018-0142-4
11379  Omoto K, Matsuda R, Nakai Y, Tatsumi Y, Nakazawa T, Tanaka Y, Shida Y, Murakami T, Nishimura F, Nakagawa I, Motoyama Y, Nakamura M, Fujimoto K, Hiroyuki N.  Expression of peptide transporter 1 has a positive correlation in protoporphyrin IX accumulation induced by 5-aminolevulinic acid with photodynamic detection of non-small cell lung cancer and metastatic brain tumor specimens originating from non-small cell lung cancer.  Photodiagnosis Photodyn Ther  2019    PubMed ID: 30639584   DOI: 10.1016/j.pdpdt.2019.01.009
14822  Hayashi T, Desmeules P, Smith RS, Drilon A, Somwar R, Ladanyi M.  RASA1 and NF1 are Preferentially Co-Mutated and Define A Distinct Genetic Subset of Smoking-Associated Non-Small Cell Lung Carcinomas Sensitive to MEK Inhibition  Clin Cancer Res  2018  24(6):1436-1447  PubMed ID: 29127119   DOI: 10.1158/1078-0432.CCR-17-2343
7050  Kajiura K, Masuda K, Naruto T, Kohmoto T, Watabnabe M, Tsuboi M, Takizawa H, Kondo K, Tangoku A, Imoto I.  Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma.  Oncotarget  2017  8:2890-2905  PubMed ID: 27926516   DOI: 10.18632/oncotarget.13761
10280  Seike M, Kim CH, Zou F, Noro R, Chiba M, Ishikawa A, Κunugi S, Kubota K, Gemma A.  AXL and GAS6 co-expression in lung adenocarcinoma as a prognostic classifier.  Oncol. Rep.  2017  37:3261-3269  PubMed ID: 28440492   DOI: 10.3892/or.2017.5594
10922  Shigeaki Umeda et al  Loss of IL-24 expression facilitates invasion in lung adenocarcinoma   Int J Clin Exp Pathol  2017  10:173-185   
14355  Okita R, Maeda A, Shimizu K, Nojima Y, Saisho S, Nakata M.  PD-L1 overexpression is partially regulated by EGFR/HER2 signaling and associated with poor prognosis in patients with non-small-cell lung cancer  Cancer Immunol Immunother   2017  66(7):865-876  PubMed ID: 28341875   DOI: 10.1007/s00262-017-1986-y
9522  Nishijima N, Seike M, Soeno C, Chiba M, Miyanaga A, Noro R, Sugano T, Matsumoto M, Kubota K, Gemma A.  miR-200/ZEB axis regulates sensitivity to nintedanib in non-small cell lung cancer cells.  Int. J. Oncol.  2016  48:937-44  PubMed ID: 26783187   DOI: 10.3892/ijo.2016.3331
12967  Okita R, Yukawa T, Nojima Y, Maeda A, Saisho S, Shimizu K, Nakata M.  MHC class I chain-related molecule A and B expression is upregulated by cisplatin and associated with good prognosis in patients with non-small cell lung cancer  Cancer Immunol Immunother  2016  65(5):499-509.  PubMed ID: 26940474   DOI: 10.1007/s00262-016-1814-9
13810  Chen S, Huang L, Sun K, Wu D, Li M, Li M, Zhong B, Chen M, Zhang S.  Enhancer of zeste homolog 2 as an independent prognostic marker for cancer: a meta-analysis  PLoS One  2015  10(5):e0125480  PubMed ID: 25974088   DOI: 10.1371/journal.pone.0125480
14314  Okita R, Wolf D, Yasuda K, Maeda A, Yukawa T, Saisho S, Shimizu K, Yamaguchi Y, Oka M, Nakayama E, Lundqvist A, Kiessling R, Seliger B, Nakata M.  Contrasting Effects of the Cytotoxic Anticancer Drug Gemcitabine and the EGFR Tyrosine Kinase Inhibitor Gefitinib on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells  PLoS One  2015  10(10):e0139809  PubMed ID: 26439264   DOI: 10.1371/journal.pone.0139809
6398  Nagano K, Yamashita T, Inoue M, Higashisaka K, Yoshioka Y, Abe Y, Mukai Y, Kamada H, Tsutsumi Y, Tsunoda S  Eph receptor A10 has a potential as a target for a prostate cancer therapy.  Biochem Biophys Res Commun  2014  450(1):545-9  PubMed ID: 24924629   DOI: 10.1016/j.bbrc.2014.06.007
13754  Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T.  Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma  Int J Oncol   2014  44(4):1118-30  PubMed ID: 24481417   DOI: 10.3892/ijo.2014.2278
15845  Kitamura K, Seike M, Okano T, Matsuda K, Miyanaga A, Mizutani H, Noro R, Minegishi Y, Kubota K, Gemma A.  MiR-134/487b/655 cluster regulates TGF-β-induced epithelial-mesenchymal transition and drug resistance to gefitinib by targeting MAGI2 in lung adenocarcinoma cells  Mol Cancer Ther  2014  13(2):444-53  PubMed ID: 24258346   DOI: 10.1158/1535-7163.MCT-13-0448
7714  Huqun, Ishikawa R, Zhang J, Miyazawa H, Goto Y, Shimizu Y, Hagiwara K, Koyama N.  Enhancer of zeste homolog 2 is a novel prognostic biomarker in nonsmall cell lung cancer.  Cancer  2012  118:1599-606  PubMed ID: 21837672   DOI: 10.1002/cncr.26441
16490  Shiba-Ishii A, Noguchi M.  Aberrant stratifin overexpression is regulated by tumor-associated CpG demethylation in lung adenocarcinoma  Am J Pathol  2012  180(4):1653-62  PubMed ID: 22310466   DOI: 10.1016/j.ajpath.2011.12.014
16538  Shimomura M, Yaoi T, Itoh K, Kato D, Terauchi K, Shimada J, Fushiki S.  Drug resistance to paclitaxel is not only associated with ABCB1 mRNA expression but also with drug accumulation in intracellular compartments in human lung cancer cell lines  Int J Oncol  2012  40(4):995-1004  PubMed ID: 22179563   DOI: 10.3892/ijo.2011.1297
16488  Shiba-Ishii A, Kano J, Morishita Y, Sato Y, Minami Y, Noguchi M.  High expression of stratifin is a universal abnormality during the course of malignant progression of early-stage lung adenocarcinoma  Int J Cancer  2011  129(10):2445-53  PubMed ID: 21207417   DOI: 10.1002/ijc.25907
5870  Takahashi N, Fukushima T, Yorita K, Tanaka H, Chijiiwa K, Kataoka H.  Dickkopf-1 is overexpressed in human pancreatic ductal adenocarcinoma cells and is involved in invasive growth.  Int J Cancer.  2010  126(7):1611-20  PubMed ID: 19711349   DOI: 10.1002/ijc.24865
15847  Mizutani H, Okano T, Minegishi Y, Matsuda K, Sudoh J, Kitamura K, Noro R, Soeno C, Yoshimura A, Seike M, Gemma A.  HSP27 modulates epithelial to mesenchymal transition of lung cancer cells in a Smad-independent manner  Oncol Lett  2010  1(6):1011-1016  PubMed ID: 22870103   DOI: 10.3892/ol.2010.190
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
13878  Tanaka Y, Goto K, Matsumoto Y, Ueoka R.  Remarkably high inhibitory effects of docosahexaenoic acid incorporated into hybrid liposomes on the growth of tumor cells along with apoptosis  Int J Pharm  2008  359(1-2):264-71  PubMed ID: 18511224   DOI: 10.1016/j.ijpharm.2008.03.045
16576  Maekawa S, Iwasaki A, Shirakusa T, Kawakami T, Yanagisawa J, Tanaka T, Shibaguchi H, Kinugasa T, Kuroki M, Kuroki M.  Association between the expression of chemokine receptors CCR7 and CXCR3, and lymph node metastatic potential in lung adenocarcinoma  Oncol Rep  2008  19(6):1461-8  PubMed ID: 18497951  
17238  Matsuda Y, Yamamoto T, Kudo M, Kawahara K, Kawamoto M, Nakajima Y, Koizumi K, Nakazawa N, Ishiwata T, Naito Z.  Expression and roles of lumican in lung adenocarcinoma and squamous cell carcinoma  Int J Oncol  2008  33(6):1177-85  PubMed ID: 19020750  
3061  Nagai, Yoshiaki, Miyazawa, Hitoshi, Tanaka, Tomoaki, Udagawa, Kiyoshi, Kato, Motoyasu, Fukuyama, Shunichiro, Yokote, Akemi, Kobayashi, Kunihiko, Kanazawa, Minoru, Hagiwara, Koichi, Hagiwara K  Genetic heterogeneity of the epidermal growth factor receptor in non-small cell lung cancer cell lines revealed by a rapid and sensitive detection system, the peptide nucleic acid-locked nucleic acid PCR clamp.  Cancer Res  2005  65:7276-82  PubMed ID: 16105816   DOI: 10.1158/0008-5472.CAN-05-0331
13836  Murai Y, Hayashi S, Takahashi H, Tsuneyama K, Takano Y.  Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines  Pathol Res Pract  2005  201(2):109-15  PubMed ID: 15901131   DOI: 10.1016/j.prp.2005.01.002
16489  Seike M, Kondo T, Fujii K, Okano T, Yamada T, Matsuno Y, Gemma A, Kudoh S, Hirohashi S.  Proteomic signatures for histological types of lung cancer  Proteomics  2005  5(11):2939-48  PubMed ID: 15996008   DOI: 10.1002/pmic.200401166
3338  Seike, Masahiro, Kondo, Tadashi, Fujii, Kazuyasu, Yamada, Tesshi, Gemma, Akihiko, Kudoh, Shoji, Hirohashi, Setsuo  Proteomic signature of human cancer cells.  Proteomics  2004  4:2776-88  PubMed ID: 15352251   DOI: 10.1002/pmic.200300795
12847  Ishii T, Teramoto S, Matsuse T.  GSTP1 affects chemoresistance against camptothecin in human lung adenocarcinoma cells  Cancer Lett  2004  216(1):89-102  PubMed ID: 15500952   DOI: 10.1016/j.canlet.2004.05.018
16491  Nakanishi H, Suda T, Katoh M, Watanabe A, Igishi T, Kodani M, Matsumoto S, Nakamoto M, Shigeoka Y, Okabe T, Oshimura M, Shimizu E.  Loss of imprinting of PEG1/MEST in lung cancer cell lines  Oncol Rep  2004  12(6):1273-8  PubMed ID: 15547750  
16486  Mase K, Iijima T, Nakamura N, Takeuchi T, Onizuka M, Mitsui T, Noguchi M.  Intrabronchial orthotopic propagation of human lung adenocarcinoma--characterizations of tumorigenicity, invasion and metastasis  Lung Cancer  2002  36(3):271-6  PubMed ID: 12009237   DOI: 10.1016/s0169-5002(02)00004-1

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