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Cell No. : Cell Name
RCB1312 : C57BL/6J-emb update : 2022/09/07 |
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| Comment | Mouse(C57BL/6J) embryonic fibroblasts cultured under the 3T3 schedule. | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Ohno, Tadao | ||
| Originator | Saijo, Kaoru | |||
| Year of deposit | 1997 | |||
| Animal | _mouse < Mammals | |||
| Strain name | C57BL/6J | |||
| Gender | Unknown | |||
| Age at sampling | embryo | |||
| Tissue | embryo/fetus, whole | |||
| Classification | semi-normal | |||
| Year of origin | 1997 | Lifespan | finite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_5U84 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM (low glucose) + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage cell No | 3 x 10 5 cells/60 mm dish |
| SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 0 | ||
| User's Publication | 2 | |||
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