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RCB1284 : B16 melanoma/lacZ  update : 2018/07/06
CommentMouse melanoma expressing E.coli lac Z. Strongly positive with beta-galactosidase.
Comment from the depositor
Use conditiona
Use condition descriptionBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Hamada, Hirofumi
Year of deposit 1996
Another name B16/lacZ
Animal mouse < Mammals
Strain name C57BL/6
Tissue skin
Case history melanoma
Classification cancer
Recombinant recombinant
Exogene MFGlacZ
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 1


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Reference
Nakamura Y, Wakimoto H, Abe J, Kanegae Y, Saito I, Aoyagi M, Hirakawa K, Hamada H  Adoptive immunotherapy with murine tumor-specific T lymphocytes engineered to secrete interleukin 2.  Cancer Res  1994  54:5757-60 

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User's Publication
Arena F, Singh JB, Gianolio E, Stefania R, Aime S.  β-Gal gene expression MRI reporter in melanoma tumor cells. Design, synthesis, and in vitro and in vivo testing of a Gd(III) containing probe forming a high relaxivity, melanin-like structure upon β-Gal enzymatic activation.  Bioconjug. Chem.  2011  22:2625-35  PubMed ID: 22035020



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