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RCB1283 : B16 melanoma  update : 2018/08/01
CommentMouse melanoma producing melanin. Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Hamada, Hirofumi
Originator Hamada, Hirofumi
Year of deposit 1996
Another name B16/parent
Animal mouse < Mammals
Strain name C57BL/6
Tissue skin
Case history melanoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 25

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547  Riley,V.  Enzymatic determination of transmissible replicating factors associated with mouse tumors.  Ann N Y Acad Sci  1963  100:762-790  PubMed ID: 13973915
587  Silagi S  Control of pigment production in mouse melanoma cells in vitro. Evocation and maintenance.  J Cell Biol  1969  43:263-74  PubMed ID: 4981070
2131  Nakamura Y, Wakimoto H, Abe J, Kanegae Y, Saito I, Aoyagi M, Hirakawa K, Hamada H  Adoptive immunotherapy with murine tumor-specific T lymphocytes engineered to secrete interleukin 2.  Cancer Res  1994  54:5757-60  PubMed ID: 7525049

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User's Publication
3410  Tamboli, Irfan Y, Prager, Kai, Barth, Esther, Heneka, Michael, Sandhoff, Konrad, Walter, Jochen  Inhibition of glycosphingolipid biosynthesis reduces secretion of the beta-amyloid precursor protein and amyloid beta-peptide.  J Biol Chem  2005  280:28110-7  PubMed ID: 15923191
7522  ET Arung, W Suwinarti, M Hendra, S Supomo, IW Kusuma, DCN Puteri, HA Eroglu, Y Kim, K Shimizu, H Ishikawa  Determination of Antioxidant and Anti-Melanogenesis Activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] Fruit Extract.  Tropical Journal of Pharmaceutical Research  2015  14:41-46 
7888  Fujii T, Ikeda K, Saito M.  Inhibitory effect of rose hip (Rosa canina L.) on melanogenesis in mouse melanoma cells and on pigmentation in brown guinea pigs.  Biosci. Biotechnol. Biochem.  2011  75:489-95  PubMed ID: 21389613
7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194
8326  Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata R.  Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. [corrected]  Transgenic Res.  2010  19:1109-17  PubMed ID: 20333465
8504  Yoshii Y, Waki A, Furukawa T, Kiyono Y, Mori T, Yoshii H, Kudo T, Okazawa H, Welch MJ, Fujibayashi Y.  Tumor uptake of radiolabeled acetate reflects the expression of cytosolic acetyl-CoA synthetase: implications for the mechanism of acetate PET.  Nucl. Med. Biol.  2009  36:771-7  PubMed ID: 19720289
8576  Yoshii Y, Furukawa T, Yoshii H, Mori T, Kiyono Y, Waki A, Kobayashi M, Tsujikawa T, Kudo T, Okazawa H, Yonekura Y, Fujibayashi Y.  Cytosolic acetyl-CoA synthetase affected tumor cell survival under hypoxia: the possible function in tumor acetyl-CoA/acetate metabolism.  Cancer Sci.  2009  100:821-7  PubMed ID: 19445015
10219  Kawata M, Taniguchi Y, Mori D, Yano F, Ohba S, Chung UI, Shimogori T, Mills AA, Tanaka S, Saito T.  Different regulation of limb development by p63 transcript variants.  PLoS ONE  2017  12:e0174122  PubMed ID: 28333962
10646  Fujimaki T, Mori S, Horikawa M, Fukui Y.  Isolation of proanthocyanidins from red wine, and their inhibitory effects on melanin synthesis in vitro.  Food Chem  2018  248:61-69  PubMed ID: 29329871
10698  Zhu WJ, Kobayashi M, Yamada K, Nishi K, Takahashi K, Mizutani A, Nishii R, Flores LG, Shikano N, Kunishima M, Kawai K.  Development of radioiodine labeled acetaminophen for specific, high-contrast imaging of malignant melanoma.  Nucl. Med. Biol.  2018  59:16-21  PubMed ID: 29413752
11043    Effect of melanogenesis inhibition by a yeast extract in comparison to that by other food extracts, and its mechanism of action  Journal of Food Biochemistry  2018  42, e12520 
4065    Bioactive isoquinoline alkaloids from Glaucium arabicum  Phytochemistry Letters  2018  8:139-144 
4066  Tomohiro Bito, Kyohei Koseki, Tomohiro Moriguchi, Yu Sasaki, Yukinori Yabuta, Tsuyoshi Ichiyanagi, Fumio Watanabe  Cycloalliin Inhibits Melanin Biosynthesis in B16 Mouse Melanoma Cells  Food Science and Technology Research  2018  24:627-633 
4073  Parichaya Aimvijarn, Teerapat Rodboon, Witchuda Payuhakrit, Prasit Suwannalert  Nymphaea pubescens Induces Apoptosis, Suppresses Cellular Oxidants-Related Cell Invasion in B16 Melanoma Cells  Pharmaceutical Sciences  2018  24, 199-206 
4152  Nishina A, Miura A, Goto M, Terakado K, Sato D, Kimura H, Hirai Y, Sato H, Phay N.  Mansonone E from Mansonia gagei Inhibited α-MSH-Induced Melanogenesis in B16 Cells by Inhibiting CREB Expression and Phosphorylation in the PI3K/Akt Pathway.  Biol. Pharm. Bull.  2018    PubMed ID: 29709914
4153  Bourhim T, Villareal MO, Gadhi C, Hafidi A, Isoda H.  Depigmenting effect of argan press-cake extract through the down-regulation of Mitf and melanogenic enzymes expression in B16 murine melanoma cells.  Cytotechnology  2018    PubMed ID: 29946948
4154  Ishihara A, Ide Y, Bito T, Ube N, Endo N, Sotome K, Maekawa N, Ueno K, Nakagiri A.  Novel tyrosinase inhibitors from liquid culture of Neolentinus lepideus.  Biosci. Biotechnol. Biochem.  2018    PubMed ID: 29297258
4155  Semba T, Sugihara E, Kamoshita N, Ueno S, Fukuda K, Yoshino M, Takao K, Yoshikawa K, Izuhara K, Arima Y, Suzuki M, Saya H.  Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma.  Cancer Sci.  2018    PubMed ID: 29498146
4156  Hatano T, Yashiro M, Fujikawa H, Motomura H.  C-X-C Motif Ligand 1 (CXCL1) from melanoma cells down-regulates the invasion of their metastatic melanoma cells.  Oncotarget  2018    PubMed ID: 30123429
4157  Aimvijarn P, Palipoch S, Okada S, Suwannalert P.  Thai Water Lily Extract Induces B16 Melanoma Cell Apoptosis and Inhibits Cellular Invasion Through the Role of Cellular Oxidants  Asian Pac. J. Cancer Prev.  2018    PubMed ID: 29373906
5400  Kawaguchi N, Noda M.  Mitf is expressed in osteoclast progenitors in vitro.  Exp Cell Res  2000  260(2):284-91  PubMed ID: 11035923
6339  Miyazaki J, Tsurui H, Kawasumi K, Kobayashi T.  Sensitivity enhancement of photothermal microscopy with radially segmented balanced detection.  Opt Lett  2015  40(4):479-82  PubMed ID: 25680129
6340  He J, Miyazaki J, Wang N, Tsurui H, Kobayashi T.  Label-free imaging of melanoma with nonlinear photothermal microscopy.  Opt Lett  2015  40(7):1141-4  PubMed ID: 25831277
6341  Miyazaki J, Tsurui H, Kawasumi K, Kobayashi T.  Simultaneous dual-wavelength imaging of nonfluorescent tissues with 3D subdiffraction photothermal microscopy.  Opt Express  2015  23(3):3647-56  PubMed ID: 25836217
6342  Hitoshi Kamauchi et al  New sesquiterpenoids isolated from Atractylodes lancea fermented by marine fungus  Tetrahedron  2015  71:1909-1914 

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