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RCB1190 : 7-TD-1  update : 2020/01/22
CommentResponding to IL-6 in the bioassay. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 23 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 23 prior to order.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor DSMZ
Year of deposit 1995
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
History overseas
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, C57B1/6
Fusion_cell_2 name myeloma
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name IL-6 dependent cell line
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 5% FBS + 0.025mM 2-Mercaptoethanol + 25U/ml IL-6
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
2034  Van Snick J, Cayphas S, Vink A, Uyttenhove C, Coulie, P G, Rubira, M R, Simpson, R J  Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas.  Proc Natl Acad Sci U S A  1986  83:9679-83  PubMed ID: 2948184

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User's Publication
9571  Fujiwara Y, Yang L, Takaiwa F, Sekikawa K.  Expression and Purification of Recombinant Mouse Interleukin-4 and -6 from Transgenic Rice Seeds.  Mol. Biotechnol.  2016  58:223-31  PubMed ID: 26876890
3790  Kishikawa H, Miura S, Yoshida H, Hirokawa M, Nakamizo H, Higuchi H, Adachi M, Nakatsumi RC, Suzuki H, Saito H, Ishii H. Author information  Transmural pressure induces IL-6 secretion by intestinal epithelial cells.  Clin Exp Immunol  2002  129(1):86-91  PubMed ID: 12100026
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265



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