Cell No. : Cell Name RCB1171 : HOUFXXX  update : 2023/09/19
Comment			Stopped distribution. This cell line has been distributed as “Human fibroblasts derived from vagina. 47,XX,+X” until August 2023. It was proved that this cell line was a misidentified cell line of HOEF (RCB1010), human fibroblasts derived from endometrium with 47,XX,+X, deposited from the same scientist.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ishiwata, Isamu
		Originator	Ishiwata, Isamu
		Year of deposit	1995
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	32 years
		Tissue	vagina
		Disease name	Trisomy X (47,XX,+X)
		Classification	normal
		Year of origin	1995
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_E957
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF12 + 15% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		47(50)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	0
		User's Publication	0

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