Cell No. : Cell Name
RCB1164 : MOLT-3
update : 2024/01/17
|
| Comment | Human T cell leukemia, the same patient as RCB0206 MOLT-4. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
DSMZ
|
| Originator |
Minowada, Jun
|
| Year of deposit |
1995
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
19 years
|
| Tissue |
blood, T-cell leukemia
|
| Classification |
cancer
|
| History |
DSMZ (ACC84)
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_0624
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Culture method |
|
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
94-100(50) : /95(5), 96(7), 97(15), 98(14), 99(5)/
|
| Surface antigen |
|
CD3(-), CD4(+,low), CD8(+)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
7
|
| User's Publication |
3
|
| Reference |
|
4748
Nakazawa S, Minowada J, Tsubota T, Sinks LF.
Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling.
Cancer Res
1978
38(6):1661-6
PubMed ID: 306283
|
|
162
Ohnuma T, Holland JF, Arkin H, Minowada J.
L-asparagine requirements of human T-lymphocytes and B-lymphocytes in culture
J Natl Cancer Inst
1977
59(4):1061-3
PubMed ID: 302863
DOI: 10.1093/jnci/59.4.1061
|
|
164
Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D.
Correlation of surface markers of cells of human lymphatic leukemias with disease type
J Natl Cancer Inst
1977
59(3):845-50
PubMed ID: 302342
DOI: 10.1093/jnci/59.3.845
|
|
160
Hayata I, Oshimura M, Minowada J, Sandberg AA.
Chromosomal banding of cultured T and B lymphocytes
In Vitro
1975
11(6):361-8.
PubMed ID: 1081484
DOI: 10.1007/BF02616372
|
|
163
Kataoka K, Minowada J, Pressman D.
Electron microscope study on human lymphocyte-sheep erythrocyte rosettes
J Natl Cancer Inst
1975
55(6):1323-5
PubMed ID: 1239515
DOI: 10.1093/jnci/55.6.1323
|
|
165
Srivastava BI, Minowada J, Moore GE.
High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin
J Natl Cancer Inst
1975
55(1):11-14
PubMed ID: 808634
DOI: 10.1093/jnci/55.1.11
|
|
494
Minowada J, Onuma T, Moore, G E
Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
J Natl Cancer Inst
1972
49:891-5
PubMed ID: 4567231
|
| User's Publication |
|
7587
Ogawa A, Akatsuka R, Tamauchi H, Hio K, Kanematsu H.
Influence of the nickel-titanium alloy components on biological functions.
BMC Proc
2011
5 Suppl 8:P79
PubMed ID: 22373219
DOI: 10.1186/1753-6561-5-S8-P79
|
|
8914
Hisakage Funabashi, Riko Matsuzawaa, Makiko Nakamura, Masayasu Mie and Eiry Kobatake
Bioluminescent enumeration of surface antigen-specific cells using the streptavidin?luciferase fusion protein
Sensors and Actuators B: Chemical
2006
120:51-56
DOI: 10.1016/j.snb.2006.01.042
|
|
15089
Kuraya M, Matsushita M, Endo Y, Thiel S, Fujita T.
Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G
Int Immunol
2003
15(1):109-17
PubMed ID: 12502731
DOI: 10.1093/intimm/dxg008
|
Back
Top
CELL BANK Website