Cell No. : Cell Name
RCB1158 : B16F10/mGM
update : 2022/07/20
|
| Comment | Mouse GM-CSF producing B16 melanoma. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Hamada, Hirofumi
|
| Originator |
Hamada, Hirofumi
|
| Year of deposit |
1995
|
| Another name |
B16F10/mGM-CSF
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6J
|
| Tissue |
skin
|
| Disease name |
melanoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
MFG-mGM-CSF
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_L292
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
0
|
| User's Publication |
5
|
| User's Publication |
|
21194
Fukaya T, Uto T, Mitoma S, Takagi H, Nishikawa Y, Tominaga M, Choijookhuu N, Hishikawa Y, Sato K.
Gut dysbiosis promotes the breakdown of oral tolerance mediated through dysfunction of mucosal dendritic cells.
Cell Rep
2023
42(5):112431
PubMed ID: 37099426
DOI: 10.1016/j.celrep.2023.112431
|
|
12087
Minh Nguyen H, Truong Nguyen H, Win N, Piow Wong C, Vu Huynh KL, Hoang NN, Do KM, Thi Nguyen H, Viet Ho D, Duc Nguyen M, Morita H.
Antimelanogenic Activity of Ocotillol-Type Saponins from Panax vietnamensis.
Chem. Biodivers.
2020
PubMed ID: 32163220
DOI: 10.1002/cbdv.202000037
|
|
4159
Win NN, Woo SY, Ngwe H, Prema, Wong CP, Ito T, Okamoto Y, Tanaka M, Imagawa H, Asakawa Y, Abe I, Morita H.
Tetrahydrofuran lignans: Melanogenesis inhibitors from Premna integrifolia wood collected in Myanmar.
Fitoterapia
2018
PubMed ID: 29540314
DOI: 10.1016/j.fitote.2018.03.002
|
|
9840
Sato K, Ando R, Kobayashi H, Nishio T.
2-Ethoxybenzamide stimulates melanin synthesis in B16F1 melanoma cells via the CREB signaling pathway.
Mol. Cell. Biochem.
2016
423:39-52
PubMed ID: 27633503
DOI: 10.1007/s11010-016-2823-x
|
|
14583
Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N.
Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease
Lab Invest
2016
96(4):468-80
PubMed ID: 26808709
DOI: 10.1038/labinvest.2015.166
|
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