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RCB1155 : Hepa-E1  update : 2018/07/31
CommentEel hepatocyte. Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kong Zwe-Ling
Year of deposit 1995
Animal eel < Fishes
Tissue liver
Classification other
History overseas
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives E-RDF + 5% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 28 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Images
deposit info
lot info
Reference information Reference 0
User's Publication 3


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Reference

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User's Publication
9912  Hiroshi Suzuki, Yuichi Ozaki, Shigeho Ijiri, Koichiro Gen, Yukinori Kazeto  17β-Hydroxysteroid Dehydrogenase Type 12a Responsible for Testicular 11-ketotestosterone Synthesis in the Japanese Eel, Anguilla Japonica  J Steroid Biochem Mol Biol  2020  198:105550  PubMed ID: 31778803
11574  Franco ME, Hill BN, Brooks BW, Lavado R.  Prymnesium parvum differentially triggers sublethal fish antioxidant responses in vitro among salinity and nutrient conditions.  Aquat. Toxicol.  2019    PubMed ID: 31185429
9477  Kotowa Sakai, Yuya Yamamoto 1, Toshitaka Ikeuchi 2  Vertebrates Originally Possess Four Functional Subtypes of G Protein-Coupled Melatonin Recepto  Sci Rep  2019  9(1):9465  PubMed ID: 31263128



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