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RCB1154 : KGN  update : 2019/04/16
CommentGranulosa cell tumor. Possible to produce progesterone after HCG addition. Cell growth is slow.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Nishi, Y. & Nawata, H.
Originator Nishi, Yoshihiro & Nawata, Hajime
Year of deposit 1995
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 69 years
Tissue ovary, peritoneum
Case history granulosa cell tumor
Classification cancer
Year of origin 1993
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 43-46(50) : 43(1),44(1),45(45),46(3)
deposit info
lot info
Reference information Reference 0
User's Publication 26

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User's Publication
7361  Diamanti-Kandarakis E, Chatzigeorgiou A, Papageorgiou E, Koundouras D, Koutsilieris M.  Advanced glycation end-products and insulin signaling in granulosa cells.  Exp. Biol. Med. (Maywood)  2015  241:1438-45  PubMed ID: 25956684
7370  Jiang C, Hou X, Wang C, May JV, Butnev VY, Bousfield GR, Davis JS.  Hypoglycosylated hFSH Has Greater Bioactivity Than Fully Glycosylated Recombinant hFSH in Human Granulosa Cells.  J. Clin. Endocrinol. Metab.  2015  100:E852-60  PubMed ID: 25915568
7417  Francois CM, Wargnier R, Petit F, Goulvent T, Rimokh R, Treilleux I, Ray-Coquard I, Zazzu V, Cohen-Tannoudji J, Guigon CJ.  17β-estradiol inhibits spreading of metastatic cells from granulosa cell tumors through a non-genomic mechanism involving GPER1.  Carcinogenesis  2015  36:564-73  PubMed ID: 25823895
7428  He C, Lv X, Hua G, Lele SM, Remmenga S, Dong J, Davis JS, Wang C.  YAP forms autocrine loops with the ERBB pathway to regulate ovarian cancer initiation and progression.  Oncogene  2015  34:6040-54  PubMed ID: 25798835
7703  Kuo FT, Bentsi-Barnes IK, Barlow GM, Pisarska MD.  Mutant Forkhead L2 (FOXL2) proteins associated with premature ovarian failure (POF) dimerize with wild-type FOXL2, leading to altered regulation of genes associated with granulosa cell differentiation.  Endocrinology  2011  152:3917-29  PubMed ID: 21862621
8127  Horling K, Santos AN, Fischer B.  The AhR is constitutively activated and affects granulosa cell features in the human cell line KGN.  Mol. Hum. Reprod.  2010  17:104-14  PubMed ID: 20823264
8239  Zhang Y, Huang Q, Cheng JC, Nishi Y, Yanase T, Huang HF, Leung PC.  Homeobox A7 increases cell proliferation by up-regulation of epidermal growth factor receptor expression in human granulosa cells.  Reprod. Biol. Endocrinol.  2010  8:61  PubMed ID: 20540809
8474  Ohno S, Yukinawa F, Noda M, Nakajin S.  Mono-(2-ethylhexyl) phthalate induces NR4A subfamily and GIOT-1 gene expression, and suppresses CYP19 expression in human granulosa-like tumor cell line KGN.  Toxicol. Lett.  2009  191:353-9  PubMed ID: 19822197
8546  Komagata S, Nakajima M, Takagi S, Mohri T, Taniya T, Yokoi T.  Human CYP24 catalyzing the inactivation of calcitriol is post-transcriptionally regulated by miR-125b.  Mol. Pharmacol.  2009  76:702-9  PubMed ID: 19570947
8888  Tsuchiya Y, Nakajima M, Takagi S, Katoh M, Zheng W, Jefcoate CR, Yokoi T.  Binding of steroidogenic factor-1 to the regulatory region might not be critical for transcriptional regulation of the human CYP1B1 gene.  J. Biochem.  2006  139:527-34  PubMed ID: 16567417
9513  Ernst J, Grabiec U, Greither T, Fischer B, Dehghani F.  The endocannabinoid system in the human granulosa cell line KGN.  Mol. Cell. Endocrinol.  2016  423:67-76  PubMed ID: 26773729
9709  Hirano M, Wada-Hiraike O, Fu H, Akino N, Isono W, Sakurabashi A, Fukuda T, Morita Y, Tanikawa M, Miyamoto Y, Nishi Y, Yanase T, Harada M, Oishi H, Yano T, Koga K, Oda K, Kawana K, Fujii T, Osuga Y.  The Emerging Role of FOXL2 in Regulating the Transcriptional Activation Function of Estrogen Receptor β: An Insight Into Ovarian Folliculogenesis.  Reprod Sci  2016    PubMed ID: 27252187
9719  Dou YD, Zhao H, Huang T, Zhao SG, Liu XM, Yu XC, Ma ZX, Zhang YC, Liu T, Gao X, Li L, Lu G, Chan WY, Gao F, Liu HB, Chen ZJ.  STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells.  Sci Rep  2016  6:26691  PubMed ID: 27270953
9805  Chao YN, Sun D, Peng YC, Wu YL.  Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells.  Int J Mol Sci  2016  17:  PubMed ID: 27548147
10083  Hoffmann M, Fiedor E, Ptak A.  Bisphenol A and its derivatives tetrabromobisphenol A and tetrachlorobisphenol A induce apelin expression and secretion in ovarian cancer cells through a peroxisome proliferator-activated receptor gamma-dependent mechanism.  Toxicol. Lett.  2017  269:15-22  PubMed ID: 28111160
10221  Colombe S, Houllier L, Fleurot E, Levallet G, Benhaïm A, Bonnamy PJ, Levallet J.  Syndecan 1 represses cell growth and FSH responsiveness in human granulosa cells.  Reproduction  2017  153:797-808  PubMed ID: 28348070
10394  Ma X, Hayes E, Biswas A, Seger C, Prizant H, Hammes SR, Sen A.  Androgens Regulate Ovarian Gene Expression Through Modulation of Ezh2 Expression and Activity.  Endocrinology  2017  158:2944-2954  PubMed ID: 28666321
10465  Hoffmann M, Gogola J, Kotula-Balak M, Ptak A.  Stimulation of ovarian cell proliferation by tetrabromobisphenol A but not tetrachlorobisphenol A through G protein-coupled receptor 30.  Toxicol In Vitro  2017  45:54-59  PubMed ID: 28811233
10723  Dang Y, Wang X, Hao Y, Zhang X, Zhao S, Ma J, Qin Y, Chen ZJ.  MicroRNA-379-5p is associate with biochemical premature ovarian insufficiency through PARP1 and XRCC6.  Cell Death Dis  2018  9:106  PubMed ID: 29367615
10810  Hoffmann M, Rak A, Ptak A.  Bisphenol A and its derivatives decrease expression of chemerin, which reverses its stimulatory action in ovarian cancer cells.  Toxicol. Lett.  2018  291:61-69  PubMed ID: 29653259
10879  Hua G, He C, Lv X, Fan L, Wang C, Remmenga SW, Rodabaugh KJ, Yang L, Lele SM, Yang P, Karpf AR, Davis JS, Wang C.  The four and a half LIM domains 2 (FHL2) regulates ovarian granulosa cell tumor progression via controlling AKT1 transcription  Cell Death Dis  2016  7:e2297  PubMed ID: 27415427
11114  Hillman RT, Celestino J, Terranova C, Beird HC, Gumbs C, Little L, Nguyen T, Thornton R, Tippen S, Zhang J, Lu KH, Gershenson DM, Rai K, Broaddus RR, Futreal PA.  KMT2D/MLL2 inactivation is associated with recurrence in adult-type granulosa cell tumors of the ovary.  Nat Commun  2018    PubMed ID: 29950560
11293  Gogola J, Hoffmann M, Ptak A.  Persistent endocrine-disrupting chemicals found in human follicular fluid stimulate the proliferation of granulosa tumor spheroids via GPR30 and IGF1R but not via the classic estrogen receptors.  Chemosphere  2019    PubMed ID: 30414542
11466  Buck T, Hack CT, Berg D, Berg U, Kunz L, Mayerhofer A.  The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells.  Sci Rep  2019    PubMed ID: 30837663
11481  Li Y, Xiang Y, Song Y, Wan L, Yu G, Tan L.  Dysregulated miR-142, -33b, and -423 in granulosa cells target TGFBR1 and SMAD7: a possible role in polycystic ovary syndrome.  Mol. Hum. Reprod.  2019    PubMed ID: 30865275
4273  Hao J, Tuck AR, Sjödin MOD, Lindberg J, Sand A, Niklasson B, Argyraki M, Hovatta O, Damdimopoulou P.  Resveratrol supports and alpha-naphthoflavone disrupts growth of human ovarian follicles in an in vitro tissue culture model.  Toxicol. Appl. Pharmacol.  2018    PubMed ID: 29146461

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