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Cell No. : Cell Name
RCB1143 : DA-1  update : 2024/01/17
CommentIL-3 dependent mouse myeloid cells. MoLV induced.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Todokoro, Kazuo
Year of deposit 1995
Animal _mouse < Mammals
Strain name BALB/c
Tissue myeloid
Disease name Moloney murine leukemia
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2015
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 10U/ml rmouse IL-3
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Images
deposit info
lot info
Reference information Reference 4
User's Publication 1


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Reference
1980  Reisbach G, Ellwart J, Dormer P  Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.  Exp Cell Res  1990  190:175-8  PubMed ID: 2209720   DOI: 10.1016/0014-4827(90)90182-a
1982  Hultner L, Szots H, Van Snick J, Moeller J, Welle M, Dormer P  Interleukin 3 mediates interleukin 6 production in murine interleukin 3-dependent hemopoietic cells.  Growth Factors  1989  2:43-51  PubMed ID: 2635055   DOI: 10.3109/08977198909069080
1981  Pope, B L, Djeu, J Y, Almawi, W Y  Secretion of a suppressor cell inducing factor by an interleukin 3-dependent cell line with natural cytotoxic activity. III. Comparison with other interleukin 3-dependent cell lines.  Cell Immunol  1988  116:1-11  PubMed ID: 2971453   DOI: 10.1016/0008-8749(88)90204-3
1978  Pierce, J H, Di Fiore, P P, Aaronson, S A, Potter M, Pumphrey J, Scott A, Ihle, J N  Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism.  Cell  1985  41:685-93  PubMed ID: 2988783   DOI: 10.1016/s0092-8674(85)80049-0

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User's Publication
4871  Feng R, Kabayama A, Uchida K, Hoshino H, Miwa M.  Cell-free entry of human T-cell leukemia virus type 1 to mouse cells.  Jpn J Cancer Res  2001  92(4):410-6  PubMed ID: 11346463   DOI: 10.1111/j.1349-7006.2001.tb01110.x



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