Cell No. : Cell Name RCB1139 : HFSKF-AE-V  update : 2022/05/12
Comment			Human fibroblasts derived from fetal skin (31 weekly age).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ishiwata, Isamu
		Originator	Ishiwata, I. & Ishikawa, H.
		Year of deposit	1994
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	embryo
		Tissue	embryo/fetus, skin
		Classification	normal
		Year of origin	1994
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_5513
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF12 + 15% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 1-2 times/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		46(50)
		STR(human)		OK
Reference information		Reference	0
		User's Publication	3

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Reference

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User's Publication

21133  Yamamoto T, Koizumi S, Oishi T, Horikawa M, Asakawa T, Yamasaki T, Sameshima T, Mitani Y, Namba H, Kurozumi K.  Migration Capacity of Stem Cells from Human Exfoliated Deciduous Teeth Towards Glioma.  J Integr Neurosci  2022  22(1):1  PubMed ID: 36722233   DOI: 10.31083/j.jin2201001

3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

3085  Shimizu, Makiko, Matsuda, Akira, Yanagisawa, Ken, Hirota, Tomomitsu, Akahoshi, Mitsuteru, Inomata, Naoko, Ebe, Kouji, Tanaka, Keiko, Sugiura, Hisashi, Nakashima, Kazuko, Tamari, Mayumi, Takahashi, Naomi, Obara, Kazuhiko, Enomoto, Tadao, Okayama, Yoshimichi, Gao, Pei-Song, Huang, Shau-Ku, Tominaga, Shin-Ichi, Ikezawa, Zenro, Shirakawa, Taro  Functional SNPs in the distal promoter of the ST2 gene are associated with atopic dermatitis.  Hum Mol Genet  2005  14:2919-27  PubMed ID: 16118232   DOI: 10.1093/hmg/ddi323