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RCB1130 : TGBC2TKB  update : 2018/07/31
CommentJapanese gallbladder carcinoma from the same patient of TGBC1TKB. Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Todoroki, T. & Ohno, T.
Originator Todoroki, T. & Ohno, T. & Kim, B.
Year of deposit 1994
Another name TGBC-2, Na
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue gallbladder
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 54-59(41) : 54(5),55(7),56(11),57(12),58(5),59(1)
deposit info
lot info
Reference information Reference 4
User's Publication 35

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3704  Targeting interleukin-4 receptor alpha by hybrid Peptide for novel biliary tract cancer therapy.  Seto 1, Shoda J, Horibe T, Warabi E, Kohno M, Yanagawa T, Bukawa H, Nakanuma Y, Kawakami K.  Int J Hepatol  2014  584650  PubMed ID: 24868471
3707  Expression of UDP-N-acetyl-alpha-D-galactosamine-polypeptide N-acetylgalactosaminyltransferase isozyme 3 in the subserosal layer correlates with postsurgical survival of pathological tumor stage 2 carcinoma of the gallbladder.  Miyahara N, Shoda J, Kawamoto T, Furukawa M, Ueda T, Todoroki T, Tanaka N, Matsuo K, Yamada Y, Kohno K, Irimura T.  Clin Cancer Res  2004  10(6):2090-9  PubMed ID: 15041730
3708  Potent in vitro and in vivo antitumor activity of interleukin-4-conjugated Pseudomonas exotoxin against human biliary tract carcinoma.  Ishige K, Shoda J, Kawamoto T, Matsuda S, Ueda T, Hyodo I, Ohkohchi N, Puri RK, Kawakami K.  Int J Cancer  2008  123(12):2915-22  PubMed ID: 18798553
3716  Expression of N-acetylglucosaminyltransferase V in the subserosal layer correlates with postsurgical survival of pathological tumor stage 2 carcinoma of the gallbladder.  Onuki K1, Sugiyama H, Ishige K, Kawamoto T, Ota T, Ariizumi S, Yamato M, Kadota S, Takeuchi K, Ishikawa A, Onodera M, Onizawa K, Yamamoto M, Miyoshi E, Shoda J.  J Gastroenterol  2014  49(4):702-14  PubMed ID: 23588887

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User's Publication
7862  Gene expression analysis for predicting gemcitabine resistance in human cholangiocarcinoma.  Sato J, Kimura T, Saito T, Anazawa T, Kenjo A, Sato Y, Tsuchiya T, Gotoh M.  J Hepatobiliary Pancreat Sci  2011  18:700-11  PubMed ID: 21451941
8464  Histone deacetylase inhibitor (SAHA) and repression of EZH2 synergistically inhibit proliferation of gallbladder carcinoma.  Yamaguchi J, Sasaki M, Sato Y, Itatsu K, Harada K, Zen Y, Ikeda H, Nimura Y, Nagino M, Nakanuma Y.  Cancer Sci.  2009  101:355-62  PubMed ID: 19860841
8548  Papillary hyperplasia of the gallbladder in pancreaticobiliary maljunction represents a senescence-related lesion induced by lysolecithin.  Yamaguchi J, Sasaki M, Harada K, Zen Y, Sato Y, Ikeda H, Itatsu K, Yokoyama Y, Ando H, Ohta T, Kubota A, Shimizu K, Nimura Y, Nagino M, Nakanuma Y.  Lab. Invest.  2009  89:1018-31  PubMed ID: 19564843
10170  Small molecule inhibitor screening identifified HSP90 inhibitor 17-AAG as potential therapeutic agent for gallbladder cancer.  Weber H, Valbuena JR, Barbhuiya MA, Stein S, Kunkel H, García P, Bizama C, Riquelme I, Espinoza JA, Kurtz SE, Tyner JW, Calderon JF, Corvalán AH, Grez M, Pandey A, Leal-Rojas P, Roa JC.  Oncotarget  2017  8:26169-26184  PubMed ID: 28412732
10222  MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer.  Yang L, Huang S, Ma H, Wu X, Feng F.  Tumour Biol.  2017  39:1010428317692249  PubMed ID: 28347246
11395  PIM1 kinase promotes gallbladder cancer cell proliferation via inhibition of proline-rich Akt substrate of 40 kDa (PRAS40).  Subbannayya T, Leal-Rojas P, Zhavoronkov A, Ozerov IV, Korzinkin M, Babu N, Radhakrishnan A, Chavan S, Raja R, Pinto SM, Patil AH, Barbhuiya MA, Kumar P, Guerrero-Preston R, Navani S, Tiwari PK, Kumar RV, Prasad TSK, Roa JC, Pandey A, Sidransky D, Gowda H, Izumchenko E, Chatterjee A.  J Cell Commun Signal  2019    PubMed ID: 30666556
3690  The ERK/MAPK pathway is overexpressed and activated in gallbladder cancer.  uchegger K, Silva R2, López J1, Ili C1, Araya JC3, Leal P2, Brebi P1, Riquelme I4, Roa JC5.  Pathol Res Pract.  2017  213(5):476-482  PubMed ID: 28285962
3691  Rapamycin and WYE-354 suppress human gallbladder cancer xenografts in mice.  Weber H, Leal P, Stein S, Kunkel H, García P, Bizama C, Espinoza JA, Riquelme I, Nervi B, Araya JC, Grez M, Roa JC.  Oncotarget  2015  6(31):31877-88  PubMed ID: 26397134
3693  AKT/mTOR substrate P70S6K is frequently phosphorylated in gallbladder cancer tissue and cell lines.  Leal P, Garcia P, Sandoval A, Buchegger K, Weber H, Tapia O, Roa JC.  Onco Targets Ther  2013  6:1373-84  PubMed ID: 24124380
3694  Inhibition of SCAMP1 suppresses cell migration and invasion in human pancreatic and gallbladder cancer cells.  Yang S, Lee KT, Lee JY, Lee JK, Lee KH, Rhee JC.  Tumour Biol  2013  34(5):2731-9  PubMed ID: 2365338
3697  Inhibitory effect of epigallocatechin-3-gallate on growth and invasion in human biliary tract carcinoma cells.  Takada M, Ku Y, Habara K, Ajiki T, Suzuki Y, Kuroda Y.  World J Surg  2002  26(6):683-6  PubMed ID: 12053219
3699  Therapeutic efficacy of G207, a conditionally replicating herpes simplex virus type 1 mutant, for gallbladder carcinoma in immunocompetent hamsters.  Nakano K, Todo T, Chijiiwa K, Tanaka M.  Mol Ther  2001  3(4):431-7.  PubMed ID: 11319903
3701  Relations of hTERT and β-Catenin Expression with Cell Growth and Invasion in Gallbladder Cell Line  DINGAng TONGSaixiong SUOTao  Clinical Medical Journal of China  2005   
3705  Overexpression of beta3/gamma2 chains of laminin-5 and MMP7 in biliary cancer.  Oka T, Yamamoto H, Sasaki S, Ii M, Hizaki K, Taniguchi H, Adachi Y, Imai K, Shinomura Y.  World J Gastroenterol  2009  15(31):3865-73  PubMed ID: 19701966
3709  Overexpression and gene amplification of EGFR, HER2, and HER3 in biliary tract carcinomas, and the possibility for therapy with the HER2-targeting antibody pertuzumab.  Kawamoto T, Ishige K, Thomas M, Yamashita-Kashima Y, Shu S, Ishikura N, Ariizumi S, Yamamoto M, Kurosaki K, Shoda J.  J Gastroenterol  2015  50(4):467-79  PubMed ID: 25112701
3711  Hepatocyte growth factor activator inhibitor type 1 suppresses metastatic pulmonary colonization of pancreatic carcinoma cells.  Fukushima T, Kawaguchi M, Yamasaki M, Tanaka H, Yorita K, Kataoka H.  Cancer Sci  2011  102(2):407-13  PubMed ID: 21166957
3712  Transcriptional silencing of Dickkopf gene family by CpG island hypermethylation in human gastrointestinal cancer.  Maehata T, Taniguchi H, Yamamoto H, Nosho K, Adachi Y, Miyamoto N, Miyamoto C, Akutsu N, Yamaoka S, Itoh F.  World J Gastroenterol  2008  14(17):2702-14  PubMed ID: 18461655
3713  Frequent epigenetic inactivation of Wnt inhibitory factor-1 in human gastrointestinal cancers.  Taniguchi H, Yamamoto H, Hirata T, Miyamoto N, Oki M, Nosho K, Adachi Y, Endo T, Imai K, Shinomura Y.  Oncogene  2005  24(53):7946-52  PubMed ID: 16007117
3714  Cancer detection by ubiquitin carboxyl-terminal esterase L1 methylation in pancreatobiliary fluids.  Kato N, Yamamoto H, Adachi Y, Ohashi H, Taniguchi H, Suzuki H, Nakazawa M, Kaneto H, Sasaki S, Imai K, Shinomura Y.  World J Gastroenterol  2013  19(11):1718-27  PubMed ID: 23555160
3717  Frequent loss of RUNX3 gene expression in human bile duct and pancreatic cancer cell lines.  Wada M, Yazumi S, Takaishi S, Hasegawa K, Sawada M, Tanaka H, Ida H, Sakakura C, Ito K, Ito Y, Chiba T.  Oncogene  2004  23(13):2401-7  PubMed ID: 14743205
3722  Insulin-like growth factor receptor expression is associated with aggressive phenotypes and has therapeutic activity in biliary tract cancers.  Ohashi H, Adachi Y, Yamamoto H, Taniguchi H, Nosho K, Suzuki H, Arimura Y, Imai K, Carbone DP, Shinomura Y.  Cancer Sci  2012  103(2):252-61  PubMed ID: 22044563
3723  Establishment and characterization of a novel highly aggressive gallbladder cancer cell line, TJ-GBC2.  Liu ZY, Xu GL, Tao HH, Yang YQ, Fan YZ.  Cancer Cell Int  2017  17:20  PubMed ID: 2819409
3724  Characteristics of a novel cell line ZJU-0430 established from human gallbladder carcinoma.  Zhou F, Zhang Y, Sun J, Yang X.  Cancer Cell Int  2019  19:190  PubMed ID: 31367188
3725  Tropomyosin-related kinase B mediated signaling contributes to the induction of malignant phenotype of gallbladder cancer.  Kawamoto M, Onishi H, Ozono K, Yamasaki A, Imaizumi A, Kamakura S, Nakano K, Oda Y, Sumimoto H, Nakamura M  Oncotarget  2017  8(22):36211-36224  PubMed ID: 28423707
3726  Dickkopf Homolog 3 (DKK3) Acts as a Potential Tumor Suppressor in Gallbladder Cancer  Kirti Gondkar, Krishna Patel, Geeta V. Patil Okaly, Bipin Nair, Akhilesh Pandey, Harsha Gowda and Prashant Kumar  Front Oncol  2019  9:1121 
3727  K-Ras mutation and amplification status is predictive of resistance and high basal pAKT is predictive of sensitivity to everolimus in biliary tract cancer cell lines.  Yeung Y, Lau DK, Chionh F, Tran H, Tse JWT, Weickhardt AJ, Nikfarjam M, Scott AM, Tebbutt NC, Mariadason JM  Mol Oncol  2017  11(9):1130-1142  PubMed ID: 28544747
3728  TrkB-mediated signaling contributes to malignant phenotypes of gallbladder cancer  Makoto Kawamoto, Hideya Onishi, Keigo Ozono, Akio Yamasaki, Akira Imaizumi and Masafumi Nakamura  AACR Annual Meeting  2017  1-5 
3729  Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer.  Subbannayya T et al  BMC Cancer  2015  15:843  PubMed ID: 26530123
3730  Gene Signature-Based Approach Identified MEK1/2 as a Potential Target Associated With Relapse After Anti-TNFα Treatment for Crohn's Disease.  Gamo K, Okuzono Y, Yabuki M, Ochi T, Sugimura K, Sato Y, Sagara M, Hayashi H, Ishimura Y, Nishimoto Y, Murakawa Y, Shiokawa Z, Gotoh M, Miyazaki T, Ebisuno Y.  Inflamm Bowel Dis  2018  24(6):1251-1265  PubMed ID: 29669006
3731  Targeting the hedgehog pathway for gallbladder cancer therapy?  Mittal B, Yadav S.  Chin Clin Oncol  2016  5(1):2  PubMed ID: 26932426
3732  Histone Deacetylase Inhibitors Inhibit the Proliferation of Gallbladder Carcinoma Cells by Suppressing AKT/mTOR Signaling.  Zhang P, Guo Z, Wu Y, Hu R, Du J, He X, Jiao X, Zhu X.  PLoS One  2015  10(8):e0136193  PubMed ID: 26287365
3738  Molecular targeting of HER2-overexpressing biliary tract cancer cells with trastuzumab emtansine, an antibody-cytotoxic drug conjugate.  Yamashita-Kashima Y, Yoshimura Y, Fujimura T, Shu S, Yanagisawa M, Yorozu K, Furugaki K, Higuchi R, Shoda J, Harada N.  Cancer Chemother Pharmacol.  2019  83(4):659-671  PubMed ID: 30659304
6101  Prostate stem cell antigen, a presumable organ-dependent tumor suppressor gene, is down-regulated in gallbladder carcinogenesis.  Ono H, Hiraoka N, Lee YS, Woo SM, Lee WJ, Choi IJ, Saito A, Yanagihara K, Kanai Y, Ohnami S, Chiwaki F, Sasaki H, Sakamoto H, Yoshida T, Saeki N.  Genes Chromosomes Cancer.  2012  51(1):30-41  PubMed ID: 21936014
6319  Alpha-methylacyl coenzyme A racemase overexpression in gallbladder carcinoma confers an independent prognostic indicator.  Wu LC, Chen LT, Tsai YJ, Lin CM, Lin CY, Tian YF, Sheu MJ, Uen YH, Shiue YL, Wang YH, Yang SJ, Wu WR, Li SH, Iwamuro M, Kobayasshi N, Huang HY, Li CF.  J Clin Pathol  2012  65(4):309-14  PubMed ID: 22267983
2673  Establishment and characterization of unique human gallbladder cancer cell lines.  Ghosh, Mila, Koike, Naoto, Yanagimoto, Go, Tsunoda, Shin-Ichi, Kaul, Sunil, Hirano, Takashi, Emura, Fabian, Kashiwagi, Hironobu, Kawamoto, Toru, Ohkohchi, Nobuhiro, Saijo, Kaoru, Ohno, Tadao, Miwa, Masanao, Todoroki, Takeshi  Int J Oncol  2004  24:1189-96  PubMed ID: 15067341

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