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RCB1127 : MC3T3-G2/PA6  update : 2018/07/06
CommentSupport hematpoietic stem cell and osteoclast differentiation. Pre-adipocyte.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kodama, Hiroaki
Originator Kodama, Hiroaki
Year of deposit 1994
Another name PA6
Original cell MC3T3-G2
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Classification other
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 51


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Reference
1846  Hormonal responsiveness of a preadipose cell line derived from newborn mouse calvaria.  Kodama, H A, Amagai Y, Koyama H, Kasai S  J Cell Physiol  1982  112:83-8  PubMed ID: 7107717
1847  A new preadipose cell line derived from newborn mouse calvaria can promote the proliferation of pluripotent hemopoietic stem cells in vitro.  Kodama, H A, Amagai Y, Koyama H, Kasai S  J Cell Physiol  1982  112:89-95  PubMed ID: 7107718

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User's Publication
2925  Fate of pre-differentiated mouse embryonic stem cells transplanted in unilaterally 6-hydroxydopamine lesioned rats: histological characterization of the grafted cells.  Thinyane K, Baier PC, Schindehutte J, Mansouri A, Paulus W, Trenkwalder C, Flugge G, Fuchs E  Brain Res  2005  1045:80-7  PubMed ID: 15910765
2274  A third-generation bisphosphonate, YM175, inhibits osteoclast formation in murine cocultures by inhibiting proliferation of precursor cells via supporting cell-dependent mechanisms.  Nishikawa M, Yamamoto M, Murakami T, Akatsu T, Kugai N, Nagata N  J Bone Miner Res  1998  13:986-95  PubMed ID: 9626630
3359  Behavioral changes in unilaterally 6-hydroxy-dopamine lesioned rats after transplantation of differentiated mouse embryonic stem cells without morphological integration.  Baier, Paul Christian, Schindehutte, Jan, Thinyane, Keneuoe, Flugge, Gabriele, Fuchs, Eberhard, Mansouri, Ahmed, Paulus, Walter, Gruss, Peter, Trenkwalder, Claudia  Stem Cells  2004  22:396-404  PubMed ID: 15153616
3373  Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells.  Pomp, Oz, Brokhman, Irina, Ben-Dor, Israel, Reubinoff, Benjamin, Goldstein, Ronald S  Stem Cells  2005  23:923-30  PubMed ID: 15883233
7759  Establishment and characterization of a fibroblast cell line derived from Mongolian sheep.  Liu CQ, Guo Y, Guan WJ, Ma YH.  Anim. Sci. J.  2011  82:215-22  PubMed ID: 21729198
7841  Varicella-zoster virus (VZV) infection of neurons derived from human embryonic stem cells: direct demonstration of axonal infection, transport of VZV, and productive neuronal infection.  Markus A, Grigoryan S, Sloutskin A, Yee MB, Zhu H, Yang IH, Thakor NV, Sarid R, Kinchington PR, Goldstein RS.  J. Virol.  2011  85:6220-33  PubMed ID: 21525353
8061  Efficient generation of nonhuman primate induced pluripotent stem cells.  Zhong B, Trobridge GD, Zhang X, Watts KL, Ramakrishnan A, Wohlfahrt M, Adair JE, Kiem HP.  Stem Cells Dev.  2010  20:795-807  PubMed ID: 21058905
8065  Efficient generation of schwann cells from human embryonic stem cell-derived neurospheres.  Ziegler L, Grigoryan S, Yang IH, Thakor NV, Goldstein RS.  Stem Cell Rev  2010  7:394-403  PubMed ID: 21052870
8107  Reversion-inducing cysteine-rich protein with Kazal motifs interferes with epidermal growth factor receptor signaling.  Kitajima S, Miki T, Takegami Y, Kido Y, Noda M, Hara E, Shamma A, Takahashi C.  Oncogene  2010  30:737-50  PubMed ID: 20890302
8281  Creating a ventral midbrain stem cell niche in an animal model for Parkinson's disease.  Fernando CV, Moses D, Parish CL, Tomas D, Drago J, Horne MK.  Stem Cells Dev.  2010  19:1995-2007  PubMed ID: 20415529
8452  Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells.  Goldstein RS, Pomp O, Brokhman I, Ziegler L.  Methods Mol. Biol.  2009  584:283-300  PubMed ID: 19907983
8480  A human neuron injury model for molecular studies of axonal regeneration.  Ziegler L, Segal-Ruder Y, Coppola G, Reis A, Geschwind D, Fainzilber M, Goldstein RS.  Exp. Neurol.  2009  223:119-27  PubMed ID: 19804775
8527  Transplantation of mouse induced pluripotent stem cells into the cochlea.  Nishimura K, Nakagawa T, Ono K, Ogita H, Sakamoto T, Yamamoto N, Okita K, Yamanaka S, Ito J.  Neuroreport  2009  20:1250-4  PubMed ID: 19625987
8719  Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus.  Ogita H, Nakagawa T, Lee KY, Inaoka T, Okano T, Kikkawa YS, Sakamoto T, Ito J.  ORL J. Otorhinolaryngol. Relat. Spec.  2008  71:32-9  PubMed ID: 18971600
8747  Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells.  Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J.  J. Neurosci. Res.  2008  86:3075-85  PubMed ID: 18543340
8863  Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.  Sato N, Brivanlou AH.  Methods Mol. Biol.  2006  331:115-28  PubMed ID: 16881513
8921  Cell transplantation to the auditory nerve and cochlear duct.  Sekiya T, Kojima K, Matsumoto M, Kim TS, Tamura T, Ito J.  Exp. Neurol.  2005  198:12-24  PubMed ID: 16376874
8938  Engraftment of embryonic stem cell-derived neurons into the cochlear modiolus.  Okano T, Nakagawa T, Endo T, Kim TS, Kita T, Tamura T, Matsumoto M, Ohno T, Sakamoto T, Iguchi F, Ito J.  Neuroreport  2005  16:1919-22  PubMed ID: 16272879
9025  Innervation of stem cell-derived neurons into auditory epithelia of mice.  Matsumoto M, Nakagawa T, Higashi T, Kim TS, Kojima K, Kita T, Sakamoto T, Ito J.  Neuroreport  2005  16:787-90  PubMed ID: 15891570
9867  Ameliorating effects of Juzentaihoto on restraint stress and P. gingivalis-induced alveolar bone loss.  Takeda O, Toyama T, Watanabe K, Sato T, Sasaguri K, Akimoto S, Sato S, Kawata T, Hamada N.  Arch. Oral Biol.  2014  59:1130-8  PubMed ID: 25064760
10023  Study of mouse induced pluripotent stem cell transplantation intoWistar albino rat cochleae after hair cell damage.  Gökcan MK, Mülazimoğlu S, Ocak E, Can P, Çalışkan M, Beşaltı Ö, Dizbay Sak S, Kaygusuz G.  Turk J Med Sci  2016  46:1603-1610  PubMed ID: 27966334
10340  Abnormal differentiation of Sandhoff disease model mouse-derived multipotent stem cells toward a neural lineage.  Ogawa Y, Kaizu K, Yanagi Y, Takada S, Sakuraba H, Oishi K.  PLoS ONE  2017  12:e0178978  PubMed ID: 28575132
10471  Ceramicines M-P from Chisocheton ceramicus: isolation and structure-activity relationship study.  Nugroho AE, Hashimoto A, Wong CP, Yokoe H, Tsubuki M, Kaneda T, Hadi AHA, Morita H.  J Nat Med  2018  72:64-72  PubMed ID: 28822030
11367  The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation.  Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K.  Mol. Cell. Endocrinol.  2019    PubMed ID: 30615908
4284  Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis.  Carr-Wilkinson J, Prathalingam N, Pal D, Moad M, Lee N, Sundaresh A, Forgham H, James P, Herbert M, Lako M, Tweddle DA.  Stem Cells Int  2018    PubMed ID: 30515222
4285  Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives.  Méndez-Maldonado K, Vega-López G, Caballero-Chacón S, Aybar MJ, Velasco I.  Int J Mol Sci  2018    PubMed ID: 30551562
4492  Stem cell colony interspacing effect on differentiation to neural cells.  Joshi R, Fuller B, Mosadegh B, Tavana H.  J Tissue Eng Regen Med  2018  12(10):2041-2054  PubMed ID: 30058271
5418  Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus.  Wong CP, Kaneda T, Hadi AH, Morita H.  J Nat Med  2014  68(1):22-30  PubMed ID: 23494817
3960  Isolation and characterization of the distal promoter region of mouse Cbfa1.  Fujiwara M, Tagashira S, Harada H, Ogawa S, Katsumata T, Nakatsuka M, Komori T, Takada H.  Biochim Biophys Acta  1999  1446(3):265-72  PubMed ID: 10524201
3964  Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation.  Nagai M, Sato N.  Biochem Biophys Res Commun  1999  257(3):719-23  PubMed ID: 10208850
3990  Intracellular calcium and protein kinase C mediate expression of receptor activator of nuclear factor-kappaB ligand and osteoprotegerin in osteoblasts.  Takami M, Takahashi N, Udagawa N, Miyaura C, Suda K, Woo JT, Martin TJ, Nagai K, Suda T.  Endocrinology  2000  141(12):4711-9  PubMed ID: 11108286
5779  Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53.  Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S.  Exp Hematol  2010  38(2):154-62  PubMed ID: 19922768
5974  Axonal growth regulation of fetal and embryonic stem cell-derived dopaminergic neurons by Netrin-1 and Slits.  Lin L1, Isacson O.  Stem Cells  2006  24(11):2504-13  PubMed ID: 16840550
5975  Selection of embryonic stem cell-derived enhanced green fluorescent protein-positive dopamine neurons using the tyrosine hydroxylase promoter is confounded by reporter gene expression in immature cell populations.  Hedlund E, Pruszak J, Ferree A, Viñuela A, Hong S, Isacson O, Kim KS.  Stem Cells  2007  25(5):1126-35  PubMed ID: 17234989
5981  Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro.  Carpenter M, Rao MS, Freed W, Zeng X.  Methods Mol Biol  2006  331:153-67.  PubMed ID: 16881516
5985  Assessment of stromal-derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells.  Vazin T, Chen J, Lee CT, Amable R, Freed WJ.  Stem Cells  2008  26(6):1517-25  PubMed ID: 18388303
5986  Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells.  Jiang X, Gwye Y, McKeown SJ, Bronner-Fraser M, Lutzko C, Lawlor ER.  Stem Cells Dev  2009  18(7):1059-70  PubMed ID: 19099373
5987  Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.  Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M.  Proc Jpn Acad Ser B Phys Biol Sci  2009  85(8):348-62.  PubMed ID: 19838014
5988  Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.  Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, Sasao M, Kubo Y, Hattori K, Saito S, Horimoto K, Yuba S, Ohgushi H.  J Biol Chem  2010  285(38):29270-8  PubMed ID: 20595386
5993  Neurons derived from human embryonic stem cells extend long-distance axonal projections through growth along host white matter tracts after intra-cerebral transplantation.  enham M, Parish CL, Leaw B, Wright J, Reid CA, Petrou S, Dottori M, Thompson LH.  Front Cell Neurosci  2012  6:11  PubMed ID: 22470319
5998  Differentiation of human embryonic stem cells into cells with corneal keratocyte phenotype.  Chan AA, Hertsenberg AJ, Funderburgh ML, Mann MM, Du Y, Davoli KA, Mich-Basso JD, Yang L, Funderburgh JL.  PLoS One  2013  8(2):e56831  PubMed ID: 23437251
6004  Generation of Corneal Keratocytes from Human Embryonic Stem Cells.  Hertsenberg AJ, Funderburgh JL.  Methods Mol Biol  2016  1341:285-94  PubMed ID: 26026882
6134  Implication of replicative stress-related stem cell ageing in radiation-induced murine leukaemia.  Ban N, Kai M  Br J Cancer.   2009  101(2):363-71  PubMed ID: 19513063
6149  Failure of transdifferentiation of adult hematopoietic stem cells into neurons.  Roybon L, Ma Z, Asztely F, Fosum A, Jacobsen SE, Brundin P, Li JY.  Stem Cells  2006  24(6):1594-604  PubMed ID: 16556707
6169  A novel combination of factors, termed SPIE, which promotes dopaminergic neuron differentiation from human embryonic stem cells.  Vazin 1, Becker KG, Chen J, Spivak CE, Lupica CR, Zhang Y, Worden L, Freed WJ.  PLoS One  2009  4(8):e6606  PubMed ID: 19672298
6173  Dopamine receptors in human embryonic stem cell neurodifferentiation.  Belinsky GS, Sirois CL, Rich MT, Short SM, Moore AR, Gilbert SE, Antic SD.  Stem Cells Dev  2013  22(10):1522-40  PubMed ID: 23286225
6208  Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease.  Ogawa Y, Tanaka M, Tanabe M, Suzuki T, Togawa T, Fukushige T, Kanekura T, Sakuraba H, Oishi K.  PLoS One  2013  8(1):e55856  PubMed ID: 23383290
6629  Generating murine osteoclasts from bone marrow.  Takahashi N, Udagawa N, Tanaka S, Suda T.  Methods Mol Med  2003  80:129-44  PubMed ID: 12728715
6634  Porphyromonas gingivalis 67-kDa fimbriae induced cytokine production and osteoclast differentiation utilizing TLR2.  Hiramine H, Watanabe K, Hamada N, Umemoto T.  FEMS Microbiol Lett  2003  229(1):49-55  PubMed ID: 14659542
6828  Insulin signaling in adipocytes differentiated from mouse stromal MC3T3-G2/PA6 cells.  Morito S, Yaguchi K, Imada M, Tachikawa C, Nomura M, Moritani S, Igarashi M, Yokogawa K, Miyamoto K.  Biol Pharm Bull  2005  28(11):2040-5  PubMed ID: 16272686
3158  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Nishimura, Haruki, Nishimura, Masahiro, Oda, Ryo, Yamanaka, Katsuyuki, Matsubara, Takehiro, Ozaki, Yoshie, Sekiya, Kensuke, Hamada, Taizo, Kato, Yukio  Exp Cell Res  2004  295:119-27  PubMed ID: 15051495



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