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RCB1127 : MC3T3-G2/PA6  update : 2018/07/06
CommentSupport hematpoietic stem cell and osteoclast differentiation. Pre-adipocyte.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kodama, Hiroaki
Originator Kodama, Hiroaki
Year of deposit 1994
Another name PA6
Original cell MC3T3-G2
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Classification other
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 27

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1846  Kodama, H A, Amagai Y, Koyama H, Kasai S  Hormonal responsiveness of a preadipose cell line derived from newborn mouse calvaria.  J Cell Physiol  1982  112:83-8 
1847  Kodama, H A, Amagai Y, Koyama H, Kasai S  A new preadipose cell line derived from newborn mouse calvaria can promote the proliferation of pluripotent hemopoietic stem cells in vitro.  J Cell Physiol  1982  112:89-95 

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User's Publication
2925  Thinyane K, Baier PC, Schindehutte J, Mansouri A, Paulus W, Trenkwalder C, Flugge G, Fuchs E  Fate of pre-differentiated mouse embryonic stem cells transplanted in unilaterally 6-hydroxydopamine lesioned rats: histological characterization of the grafted cells.  Brain Res  2005  1045:80-7  PubMed ID: 15910765
2274  Nishikawa M, Yamamoto M, Murakami T, Akatsu T, Kugai N, Nagata N  A third-generation bisphosphonate, YM175, inhibits osteoclast formation in murine cocultures by inhibiting proliferation of precursor cells via supporting cell-dependent mechanisms.  J Bone Miner Res  1998  13:986-95  PubMed ID: 9626630
3359  Baier, Paul Christian, Schindehutte, Jan, Thinyane, Keneuoe, Flugge, Gabriele, Fuchs, Eberhard, Mansouri, Ahmed, Paulus, Walter, Gruss, Peter, Trenkwalder, Claudia  Behavioral changes in unilaterally 6-hydroxy-dopamine lesioned rats after transplantation of differentiated mouse embryonic stem cells without morphological integration.  Stem Cells  2004  22:396-404  PubMed ID: 15153616
3468  Pomp, Oz, Brokhman, Irina, Ben-Dor, Israel, Reubinoff, Benjamin, Goldstein, Ronald S  Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells.  Stem Cells  2005  23:923-30  PubMed ID: 15883233
7759  Liu CQ, Guo Y, Guan WJ, Ma YH.  Establishment and characterization of a fibroblast cell line derived from Mongolian sheep.  Anim. Sci. J.  2011  82:215-22  PubMed ID: 21729198
7841  Markus A, Grigoryan S, Sloutskin A, Yee MB, Zhu H, Yang IH, Thakor NV, Sarid R, Kinchington PR, Goldstein RS.  Varicella-zoster virus (VZV) infection of neurons derived from human embryonic stem cells: direct demonstration of axonal infection, transport of VZV, and productive neuronal infection.  J. Virol.  2011  85:6220-33  PubMed ID: 21525353
8018  Preynat-seauve, Olivier (Habare-Lullin, FR)Krause, Karl-heinz (Geneva, CH)  SELECTION OF STEM CELL CLONES WITH DEFINED DIFFERENTIATION CAPABILITIES  United States Patent  2011  :20110136681 
8061  Zhong B, Trobridge GD, Zhang X, Watts KL, Ramakrishnan A, Wohlfahrt M, Adair JE, Kiem HP.  Efficient generation of nonhuman primate induced pluripotent stem cells.  Stem Cells Dev.  2010  20:795-807  PubMed ID: 21058905
8065  Ziegler L, Grigoryan S, Yang IH, Thakor NV, Goldstein RS.  Efficient generation of schwann cells from human embryonic stem cell-derived neurospheres.  Stem Cell Rev  2010  7:394-403  PubMed ID: 21052870
8107  Kitajima S, Miki T, Takegami Y, Kido Y, Noda M, Hara E, Shamma A, Takahashi C.  Reversion-inducing cysteine-rich protein with Kazal motifs interferes with epidermal growth factor receptor signaling.  Oncogene  2010  30:737-50  PubMed ID: 20890302
8281  Fernando CV, Moses D, Parish CL, Tomas D, Drago J, Horne MK.  Creating a ventral midbrain stem cell niche in an animal model for Parkinson's disease.  Stem Cells Dev.  2010  19:1995-2007  PubMed ID: 20415529
8452  Goldstein RS, Pomp O, Brokhman I, Ziegler L.  Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells.  Methods Mol. Biol.  2009  584:283-300  PubMed ID: 19907983
8480  Ziegler L, Segal-Ruder Y, Coppola G, Reis A, Geschwind D, Fainzilber M, Goldstein RS.  A human neuron injury model for molecular studies of axonal regeneration.  Exp. Neurol.  2009  223:119-27  PubMed ID: 19804775
8527  Nishimura K, Nakagawa T, Ono K, Ogita H, Sakamoto T, Yamamoto N, Okita K, Yamanaka S, Ito J.  Transplantation of mouse induced pluripotent stem cells into the cochlea.  Neuroreport  2009  20:1250-4  PubMed ID: 19625987
8719  Ogita H, Nakagawa T, Lee KY, Inaoka T, Okano T, Kikkawa YS, Sakamoto T, Ito J.  Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus.  ORL J. Otorhinolaryngol. Relat. Spec.  2008  71:32-9  PubMed ID: 18971600
8747  Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J.  Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells.  J. Neurosci. Res.  2008  86:3075-85  PubMed ID: 18543340
8863  Sato N, Brivanlou AH.  Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.  Methods Mol. Biol.  2006  331:115-28  PubMed ID: 16881513
8921  Sekiya T, Kojima K, Matsumoto M, Kim TS, Tamura T, Ito J.  Cell transplantation to the auditory nerve and cochlear duct.  Exp. Neurol.  2005  198:12-24  PubMed ID: 16376874
8938  Okano T, Nakagawa T, Endo T, Kim TS, Kita T, Tamura T, Matsumoto M, Ohno T, Sakamoto T, Iguchi F, Ito J.  Engraftment of embryonic stem cell-derived neurons into the cochlear modiolus.  Neuroreport  2005  16:1919-22  PubMed ID: 16272879
9025  Matsumoto M, Nakagawa T, Higashi T, Kim TS, Kojima K, Kita T, Sakamoto T, Ito J.  Innervation of stem cell-derived neurons into auditory epithelia of mice.  Neuroreport  2005  16:787-90  PubMed ID: 15891570
9867  Takeda O, Toyama T, Watanabe K, Sato T, Sasaguri K, Akimoto S, Sato S, Kawata T, Hamada N.  Ameliorating effects of Juzentaihoto on restraint stress and P. gingivalis-induced alveolar bone loss.  Arch. Oral Biol.  2014  59:1130-8  PubMed ID: 25064760
10023  Gökcan MK, Mülazimoğlu S, Ocak E, Can P, Çalışkan M, Beşaltı Ö, Dizbay Sak S, Kaygusuz G.  Study of mouse induced pluripotent stem cell transplantation intoWistar albino rat cochleae after hair cell damage.  Turk J Med Sci  2016  46:1603-1610  PubMed ID: 27966334
10471  Nugroho AE, Hashimoto A, Wong CP, Yokoe H, Tsubuki M, Kaneda T, Hadi AHA, Morita H.  Ceramicines M-P from Chisocheton ceramicus: isolation and structure-activity relationship study.  J Nat Med  2018  72:64-72  PubMed ID: 28822030
11367  Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K.  The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation.  Mol. Cell. Endocrinol.  2019    PubMed ID: 30615908
4284  Carr-Wilkinson J, Prathalingam N, Pal D, Moad M, Lee N, Sundaresh A, Forgham H, James P, Herbert M, Lako M, Tweddle DA.  Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis.  Stem Cells Int  2018    PubMed ID: 30515222
4285  Méndez-Maldonado K, Vega-López G, Caballero-Chacón S, Aybar MJ, Velasco I.  Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives.  Int J Mol Sci  2018    PubMed ID: 30551562
4492  Joshi R, Fuller B, Mosadegh B, Tavana H.  Stem cell colony interspacing effect on differentiation to neural cells.  J Tissue Eng Regen Med  2018  12(10):2041-2054  PubMed ID: 30058271

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