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RCB1124 : OP9  update : 2018/07/06
CommentSupport hematpoietic stem cell and ES cell differentiation to blood cells and lymphocytes.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. For commercial use, please contact RegCell Co., Ltd.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kodama, H. & Nakano, T.
Originator Kodama, Hiroaki
Year of deposit 1994
Animal mouse < Mammals
Strain name B6C3Fe-a/a, op
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Genetics op/op
Classification other
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 20% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP (mouse) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 29


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Reference

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User's Publication
3411  de Pooter, Renee F, Schmitt, Thomas M, de la Pompa, Jose Luis, Fujiwara, Yuko, Orkin, Stuart H, Zuniga-Pflucker, Juan Carlos  Notch signaling requires GATA-2 to inhibit myelopoiesis from embryonic stem cells and primary hemopoietic progenitors.  J Immunol  2006  176:5267-75  PubMed ID: 16621992
2428  Okada S, Yoshida T, Hong Z, Ishii G, Hatano M, Kuro-O M, Nabeshima Y, Nabeshima Y, Tokuhisa T  Impairment of B lymphopoiesis in precocious aging (klotho) mice.  Int Immunol  2000  12:861-71  PubMed ID: 10837414
2666  Kawamura, Toshihiko, Koka, Rima, Ma, Averil, Kumar, Vinay  Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction.  J Immunol  2003  171:5085-90  PubMed ID: 14607906
3171  Niida, Shumpei, Kondo, Takako, Hiratsuka, Sachie, Hayashi, Shin-Ichi, Amizuka, Norio, Noda, Tetsuo, Ikeda, Kyoji, Shibuya, Masabumi  VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice.  Proc Natl Acad Sci U S A  2005  102:14016-21  PubMed ID: 16172397
7000  Wakabayashi T, Naito H, Suehiro JI, Lin Y, Kawaji H, Iba T, Kouno T, Ishikawa-Kato S, Furuno M, Takara K, Muramatsu F, Weizhen J, Kidoya H, Ishihara K, Hayashizaki Y, Nishida K, Yoder MC, Takakura N.  CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties.  Cell Stem Cell  2018    PubMed ID: 29429943
7053  Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.  Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.  Cancer Res.  2016  76:6839-6850  PubMed ID: 27872100
7479  Kato S, Kato Y, Shibata H, Saitoh Y, Miwa N.  Repressive effects of oat extracts on intracellular lipid-droplet formation in adipocytes and a three-dimensional subcutaneous adipose tissue model.  Mater Sci Eng C Mater Biol Appl  2015  49:269-73  PubMed ID: 25686949
7573  Yoshimitsu M, Higuchi K, Arima N, Medin JA, Takenaka T  Successful Induction of Pluripotent Stem Cells From a Fabry Disease Mouse Model: Toward the Development of Safe Lentiviral Gene Therapy.  J Stem Cell Res Ther  2015  5:1000259 
8040  Nishimura K, Sano M, Ohtaka M, Furuta B, Umemura Y, Nakajima Y, Ikehara Y, Kobayashi T, Segawa H, Takayasu S, Sato H, Motomura K, Uchida E, Kanayasu-Toyoda T, Asashima M, Nakauchi H, Yamaguchi T, Nakanishi M.  Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming.  J. Biol. Chem.  2010  286:4760-71  PubMed ID: 21138846
8154  Sumitomo A, Ishino R, Urahama N, Inoue K, Yonezawa K, Hasegawa N, Horie O, Matsuoka H, Kondo T, Roeder RG, Ito M.  The transcriptional mediator subunit MED1/TRAP220 in stromal cells is involved in hematopoietic stem/progenitor cell support through osteopontin expression.  Mol. Cell. Biol.  2010  30:4818-27  PubMed ID: 20713445
8233  Mohtashami M, Shah DK, Nakase H, Kianizad K, Petrie HT, Zuniga-Pflucker JC.  Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes.  J. Immunol.  2010  185:867-76  PubMed ID: 20548034
8250  Xiao L, Aoshima H, Saitoh Y, Miwa N.  The effect of squalane-dissolved fullerene-C60 on adipogenesis-accompanied oxidative stress and macrophage activation in a preadipocyte-monocyte co-culture system.  Biomaterials  2010  31:5976-85  PubMed ID: 20488530
8423  Suzuki N, Itou T, Hasegawa Y, Okazaki T, Ikeno M.  Cell to cell transfer of the chromatin-packaged human beta-globin gene cluster.  Nucleic Acids Res.  2009  38:e33  PubMed ID: 20007595
8722  Nagao K, Ohta T, Hinohara A, Tahara T, Hagiwara T, Maeda Y, Yoneya T, Sohma Y, Heike T, Nakahata T, Inagaki Y, Nishikawa M.  Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis.  Biochem. Biophys. Res. Commun.  2008  377:205-9  PubMed ID: 18840400
9954  Morgan BJ, Crabtree D, Skatrud JB.  Chemoreflex sensitization augments sympathetic vasomotor outflow in awake humans.  Adv. Exp. Med. Biol.  1994  360:269-71  PubMed ID: 7872100
10017  Tanaka S, Maekawa A, Matsubara L, Imanishi A, Yano M, Roeder RG, Hasegawa N, Asano S, Ito M.  Periostin supports hematopoietic progenitor cells and niche-dependent myeloblastoma cells in vitro.  Biochem. Biophys. Res. Commun.  2016  478:1706-12  PubMed ID: 27596966
10294  Nishimura T, Unezaki N, Kanegi R, Wijesekera DPH, Hatoya S, Sugiura K, Kawate N, Tamada H, Imai H, Inaba T.  Generation of Canine Induced Extraembryonic Endoderm-Like Cell Line That Forms Both Extraembryonic and Embryonic Endoderm Derivatives.  Stem Cells Dev.  2017  26:1111-1120  PubMed ID: 28474540
10826  Tadokoro Y, Hoshii T, Yamazaki S, Eto K, Ema H, Kobayashi M, Ueno M, Ohta K, Arai Y, Hara E, Harada K, Oshima M, Oshima H, Arai F, Yoshimura A, Nakauchi H, Hirao A.  Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress.  Cell Stem Cell  2018  22:713-725.e8  PubMed ID: 29706577
4047  Naito H, Iba T, Wakabayashi T, Tai-Nagara I, Suehiro JI, Jia W, Eino D, Sakimoto S, Muramatsu F, Kidoya H, Sakurai H, Satoh T, Akira S, Kubota Y, Takakura N.  TAK1 Prevents Endothelial Apoptosis and Maintains Vascular Integrity  Dev Cell  2019  48(2):151-166  PubMed ID: 30639056
5454  Kudo H, Wada H, Sasaki H, Tsuji H, Otsuka R, Baghdadi M, Kojo S, Chikaraishi T, Seino K.  Induction of macrophage-like immunosuppressive cells from mouse ES cells that contribute to prolong allogeneic graft survival.  PLoS One.  2014  9(10):e111826  PubMed ID: 25356669
4000  Okada A, Tashiro K, Yamaguchi T, Kawabata K.  Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers.  Methods Mol Biol  2016  1341:181-95  PubMed ID: 26138986
3948  Tashiro K, Hirata N, Okada A, Yamaguchi T, Takayama K, Mizuguchi H, Kawabata K.  Expression of coxsackievirus and adenovirus receptor separates hematopoietic and cardiac progenitor cells in fetal liver kinase 1-expressing mesoderm.  Stem Cells Transl Med  2015  4(5):424-36  PubMed ID: 25762001
5989  Tanaka Y, Era T, Nishikawa S, Kawamata S.  Forced expression of Nanog in hematopoietic stem cells results in a gammadeltaT-cell disorder.  Blood  2007  110(1):107-15  PubMed ID: 17360937
6066  Awong G, Zúñiga-Pflücker JC.  Generation, isolation, and engraftment of in vitro-derived human T cell progenitors.  Methods Mol Biol  2013  946:103-13  PubMed ID: 23179828
6084  Kato S, Saitoh Y, Miwa N.  Repressive effects of a capacitive-resistive electric transfer (CRet) hyperthermic apparatus combined with provitamin C on intracellular lipid-droplets formation in adipocytes.  Int J Hyperthermia  2013  29(1):30-7  PubMed ID: 23286696
6130  Holmes R, Zúñiga-Pflücker JC.  The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro.  Cold Spring Harb Protoc. 2009 Feb  2009  2009(2):pdb.prot5156  PubMed ID: 20147086
6137  Awong G1, La Motte-Mohs RN, Zúñiga-Pflücker JC.  In vitro human T cell development directed by notch-ligand interactions.  Methods Mol Biol  2008  430:135-42  PubMed ID: 18370296
3357  Hiroyama, Takashi, Miharada, Kenichi, Aoki, Naoko, Fujioka, Tsuyoshi, Sudo, Kazuhiro, Danjo, Inaho, Nagasawa, Toshiro, Nakamura, Yukio  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp Hematol  2006  34:760-9  PubMed ID: 16728281
3358  Takayanagi, Shin-ichiro, Hiroyama, Takashi, Yamazaki, Satoshi, Nakajima, Tomoko, Morita, Yohei, Usui, Joichi, Eto, Koji, Motohashi, Tsutomu, Shiomi, Kensuke, Keino-Masu, Kazuko, Masu, Masayuki, Oike, Yuichi, Mori, Shigeo, Yoshida, Nobuaki, Iwama, Atsushi, Nakauchi, Hiromitsu  Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell-surface protein with the thrombospondin-1 domain.  Blood  2006  107:4317-25  PubMed ID: 16455951



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