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RCB1089 : ψCRIP-MFGmGM-CSF  update : 2018/07/06
CommentProducer of MoMLV-derived retrovirus including mouse GM-CSF cDNA
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Hamada, Hirofumi
Originator Hamada, Hirofumi
Year of deposit 1994
Original cell ψCRIP
Animal mouse < Mammals
Strain name NIH Swiss
Genus Mus musculus
Species mus domesticus
Age at sampling embryo
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene Amp^r, Mouse GM-CSF cDNA
Vbno V00002
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Reference information Reference 0
User's Publication 0


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