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RCB1034 : CJM  update : 2018/07/06
CommentHuman cell line derived from metastasis of cancer occurred in oral cavity, the same patient as T3M-1 Clone2 and T3M-1 Cl-10.
Comment from the depositor
Use conditiona
Use condition descriptionBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name CJM-108
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue oral, the thoracic cavity meta
Metastastatic ability Yes
Metastatsd tissue the thoracic cavity
Classification cancer
Year of origin 1977
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Chromosome mode 61-69(50) : /63(5),64(7),65(7),66(9),67(9),68(8)/
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
Nishizawa T, Nakano K, Harada A, Kakiuchi M, Funahashi SI, Suzuki M, Ishikawa S, Aburatani H.  DGC-specific <i>RHOA</i> mutations maintained cancer cell survival and promoted cell migration via ROCK inactivation.  Oncotarget  2018    PubMed ID: 29796182



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