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RCB1027 : MEB4  update : 2018/07/06
CommentNot expressing melanoma antigen. Control for GM-95 cell line.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
RIKEN Center for Brain Science

Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Dr.Hirabayashi, Yoshio
Fax. +81-48-467-6372
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Hirabayashi, Yoshio
Originator Sakiyama, Hisako
Year of deposit 1994
Original cell B16 melanoma
Animal mouse < Mammals
Strain name C57BL/6J
Tissue skin
Genetics melanoma
Classification cancer
History Yakiyama, Hisako - Hirabayashi, Yoshio
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 4


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Reference
1893  Ichikawa S, Nakajo N, Sakiyama H, Hirabayashi Y  A mouse B16 melanoma mutant deficient in glycolipids.  Proc Natl Acad Sci U S A  1994  91:2703-7  PubMed ID: 8146177
1892  Nozue M, Sakiyama H, Tsuchiya K, Hirabayashi Y, Taniguchi M  Melanoma antigen expression and metastatic ability of mutant B16 melanoma clones.  Int J Cancer  1988  42:734-8  PubMed ID: 3053466

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User's Publication
9665  Szerencsei RT, Ginger RS, Green MR, Schnetkamp PP.  Identification and Characterization of K(+)-Dependent Na(+)-Ca(2+) Exchange Transport in Pigmented MEB4 Cells Mediated by NCKX4.  Biochemistry  2016  55:2704-12  PubMed ID: 27093457
7729  van der Poel S, Wolthoorn J, van den Heuvel D, Egmond M, Groux-Degroote S, Neumann S, Gerritsen H, van Meer G, Sprong H.  Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity.  Traffic  2011  12:1634-47  PubMed ID: 21810155
2655  Matsushima-Hibiya, Yuko, Watanabe, Masahiko, Hidari, Kazuya I-P, Miyamoto, Daisei, Suzuki, Yasuo, Kasama, Takeshi, Kasama, Takashi, Koyama, Kotaro, Sugimura, Takashi, Wakabayashi, Keiji  Identification of glycosphingolipid receptors for pierisin-1, a guanine-specific ADP-ribosylating toxin from the cabbage butterfly.  J Biol Chem  2003  278:9972-8  PubMed ID: 12645583
2558  Kanazawa T, Watanabe M, Matsushima-Hibiya Y, Kono T, Tanaka N, Koyama K, Sugimura T, Wakabayashi K  Distinct roles for the N- and C-terminal regions in the cytotoxicity of pierisin-1, a putative ADP-ribosylating toxin from cabbage butterfly, against mammalian cells.  Proc Natl Acad Sci U S A  2001  98:2226-31  PubMed ID: 11226221



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