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RCB1027 : MEB4  update : 2018/07/06
CommentNot expressing melanoma antigen. Control for GM-95 cell line.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN Center for Brain Science

Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Dr.Hirabayashi, Yoshio
Fax. +81-48-467-6372
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Hirabayashi, Yoshio
Originator Sakiyama, Hisako
Year of deposit 1994
Original cell B16 melanoma
Animal mouse < Mammals
Strain name C57BL/6J
Tissue skin
Genetics melanoma
Classification cancer
History Yakiyama, Hisako - Hirabayashi, Yoshio
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 4

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1893  Ichikawa S, Nakajo N, Sakiyama H, Hirabayashi Y  A mouse B16 melanoma mutant deficient in glycolipids.  Proc Natl Acad Sci U S A  1994  91:2703-7  PubMed ID: 8146177
1892  Nozue M, Sakiyama H, Tsuchiya K, Hirabayashi Y, Taniguchi M  Melanoma antigen expression and metastatic ability of mutant B16 melanoma clones.  Int J Cancer  1988  42:734-8  PubMed ID: 3053466

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User's Publication
9665  Szerencsei RT, Ginger RS, Green MR, Schnetkamp PP.  Identification and Characterization of K(+)-Dependent Na(+)-Ca(2+) Exchange Transport in Pigmented MEB4 Cells Mediated by NCKX4.  Biochemistry  2016  55:2704-12  PubMed ID: 27093457
7729  van der Poel S, Wolthoorn J, van den Heuvel D, Egmond M, Groux-Degroote S, Neumann S, Gerritsen H, van Meer G, Sprong H.  Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity.  Traffic  2011  12:1634-47  PubMed ID: 21810155
2655  Matsushima-Hibiya, Yuko, Watanabe, Masahiko, Hidari, Kazuya I-P, Miyamoto, Daisei, Suzuki, Yasuo, Kasama, Takeshi, Kasama, Takashi, Koyama, Kotaro, Sugimura, Takashi, Wakabayashi, Keiji  Identification of glycosphingolipid receptors for pierisin-1, a guanine-specific ADP-ribosylating toxin from the cabbage butterfly.  J Biol Chem  2003  278:9972-8  PubMed ID: 12645583
2558  Kanazawa T, Watanabe M, Matsushima-Hibiya Y, Kono T, Tanaka N, Koyama K, Sugimura T, Wakabayashi K  Distinct roles for the N- and C-terminal regions in the cytotoxicity of pierisin-1, a putative ADP-ribosylating toxin from cabbage butterfly, against mammalian cells.  Proc Natl Acad Sci U S A  2001  98:2226-31  PubMed ID: 11226221

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