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RCB1026 : GM95  update : 2018/07/06
CommentGlucosylceramide synthetase-deficient B16 melanoma mutant.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN Center for Brain Science

Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Dr.Hirabayashi, Yoshio
Fax. +81-48-467-6372
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Hirabayashi, Yoshio
Originator Sakiyama, Hisako
Year of deposit 1994
Animal mouse < Mammals
Strain name C57BL/6J
Tissue skin
Case history B16 melanoma mutant
Genetics glucosylceramide synthetase deficient
Classification mutant
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
deposit info
lot info
Reference information Reference 1
User's Publication 6

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1892  Nozue M, Sakiyama H, Tsuchiya K, Hirabayashi Y, Taniguchi M  Melanoma antigen expression and metastatic ability of mutant B16 melanoma clones.  Int J Cancer  1988  42:734-8  PubMed ID: 3053466

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User's Publication
9471  Nobumasa Takasugi, Tomoki Sasaki, Mitsuru Shinohara, Takeshi Iwatsubo, Taisuke Tomita   Synthetic Ceramide Analogues Increase Amyloid-β 42 Production by Modulating γ-Secretase Activity  Biochem Biophys Res Commun  2015  457(2):194-9  PubMed ID: 25545059
9103  Lorena Sánchez-Felipe, Enrique Villar, Isabel Muñoz-Barroso  Entry of Newcastle Disease Virus Into the Host Cell: Role of Acidic pH and Endocytosis  Biochim Biophys Acta  2014  1838 (1 Pt B), 300-9  PubMed ID: 23994097
7729  van der Poel S, Wolthoorn J, van den Heuvel D, Egmond M, Groux-Degroote S, Neumann S, Gerritsen H, van Meer G, Sprong H.  Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity.  Traffic  2011  12:1634-47  PubMed ID: 21810155
7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194
3410  Tamboli, Irfan Y, Prager, Kai, Barth, Esther, Heneka, Michael, Sandhoff, Konrad, Walter, Jochen  Inhibition of glycosphingolipid biosynthesis reduces secretion of the beta-amyloid precursor protein and amyloid beta-peptide.  J Biol Chem  2005  280:28110-7  PubMed ID: 15923191
9031  S Ablan, S S Rawat, R Blumenthal, A Puri  Entry of Influenza Virus Into a Glycosphingolipid-Deficient Mouse Skin Fibroblast Cell Line  Arch Virol  2001  146 (11), 2227-38  PubMed ID: 11765924

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