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RCB1005 : KP4  update : 2018/07/06
CommentPancreatic ductal cell carcinoma. Mother cell line of KP4-1, -2, and -3.
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nishi, Y. & Nawata, H.
Originator Nawata, H. & Nishi, Y.
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 50 years
Tissue pancreas, ascites meta
Case history pancreatic ductal cell carcino
Metastastatic ability Yes
Metastatsd tissue liver, lung, etc
Classification cancer
Year of origin 1990
History Direct from originator.
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Chromosome mode 62-174(50) : /64(6),65(6),67(8),69(6),70(5),71(2)/
Images
deposit info
lot info
Reference information Reference 1
User's Publication 11


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Reference
Nishi, Y., Haji, M., Takayanagi, R., Iguchi, H., Shimazoe, T., Hirata, J., and Nawata, H.  Establishment and characterization of PTHrP-producing human pancreatic cancer cell line  Int J Oncol  1994  5:33-39 

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User's Publication
Takeda, Ami, Osaki, Mitsuhiko, Adachi, Keiko, Honjo, Soichiro, Ito, Hisao  Role of the phosphatidylinositol 3'-kinase-Akt signal pathway in the proliferation of human pancreatic ductal carcinoma cell lines.  Pancreas  2004  28:353-8  PubMed ID: 15084985
Kawakubo K, Ohnishi S, Hatanaka Y, Hatanaka KC, Hosono H, Kubota Y, Kamiya M, Kuwatani M, Kawakami H, Urano Y, Sakamoto N.  Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study.  Mol Imaging Biol  2015  18:463-71  PubMed ID: 26431952
Miwa T, Kokuryo T, Yokoyama Y, Yamaguchi J, Nagino M.  Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer.  Cancer Med  2015  4:1091-100  PubMed ID: 25914189
Seki T, Kokuryo T, Yokoyama Y, Suzuki H, Itatsu K, Nakagawa A, Mizutani T, Miyake T, Uno M, Yamauchi K, Nagino M.  Antitumor effects of α-bisabolol against pancreatic cancer.  Cancer Sci.  2011  102:2199-205  PubMed ID: 21883695
Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550
Jin H, Yang R, Zheng Z, Romero M, Ross J, Bou-Reslan H, Carano RA, Kasman I, Mai E, Young J, Zha J, Zhang Z, Ross S, Schwall R, Colbern G, Merchant M.  MetMAb, the one-armed 5D5 anti-c-Met antibody, inhibits orthotopic pancreatic tumor growth and improves survival.  Cancer Res.  2008  68:4360-8  PubMed ID: 18519697
Sellmann C, Doerner A, Knuehl C, Rasche N, Sood V, Krah S, Rhiel L, Messemer A, Wesolowski J, Schuette M, Becker S, Toleikis L, Kolmar H, Hock B.  Balancing Selectivity and Efficacy of Bispecific Epidermal Growth Factor Receptor (EGFR) × c-MET Antibodies and Antibody-Drug Conjugates.  J. Biol. Chem.  2016  291:25106-25119  PubMed ID: 27694443
Wong M, Funasaka K, Obayashi T, Miyahara R, Hirooka Y, Hamaguchi M, Goto H, Senga T.  AMPD3 is associated with the malignant characteristics of gastrointestinal stromal tumors.  Oncol Lett  2017  13:1281-1287  PubMed ID: 28454247
Murata Y, Kokuryo T, Yokoyama Y, Yamaguchi J, Miwa T, Shibuya M, Yamamoto Y, Nagino M.  The Anticancer Effects of Novel α-Bisabolol Derivatives Against Pancreatic Cancer.  Anticancer Res.  2017  37:589-598  PubMed ID: 28179305
Kania E, Pająk B, O'Prey J, Sierra Gonzalez P, Litwiniuk A, Urbańska K, Ryan KM, Orzechowski A.  Verapamil treatment induces cytoprotective autophagy by modulating cellular metabolism.  FEBS J.  2017  284:1370-1387  PubMed ID: 28342290
  Population Pharmacokinetics of Liposomal Irinotecan  CLINICAL PHARMACOLOGY & THERAPEUTICS  2017  102 



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