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RCB0995 : MDCK  update : 2019/12/11
CommentCanine kidney epithelial. Sensitive to wide range of viruses. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA84121903 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA84121903 prior to order.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Originator Madin, S. H. & Darby, N. B.
Year of deposit 1994
Animal dog < Mammals
Genus Canis
Species familiaris
Tissue kidney
Classification other
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 53


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Reference
2581  Madin-Darby canine kidney cells. II. Aldosterone stimulates Na+/H+ and Cl-/HCO3- exchange.  Oberleithner H, Vogel U, Kersting U, Steigner W  Pflugers Arch  1990  416:533-9  PubMed ID: 2172916
260  Characterization of an established line of canine kidney cells (MDCK)  Gaush CR, Hard WL, Smith TF.  Proc Soc Exp Biol Med.  1966  122(3):931-5  PubMed ID: 5918973

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User's Publication
2636  Inhibitory role of neutrophils on influenza virus multiplication in the lungs of mice.  Fujisawa H  Microbiol Immunol  2001  45:679-88  PubMed ID: 11762750
3080  Experimental dual infection of pigs with an H1N1 swine influenza virus (A/Sw/Hok/2/81) and Mycoplasma hyopneumoniae.  Yazawa S, Okada M, Ono M, Fujii S, Okuda Y, Shibata I, Kida H  Vet Microbiol  2004  98:221-8  PubMed ID: 15036530
7058  Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss.  Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.  EMBO Mol Med  2016  8:1310-1324  PubMed ID: 27707755
7126  Presence of an H(+)/Quinidine Antiport System in Madin-Darby Canine Kidney Cells.  Fukao M, Kondo E, Nishino H, Hattori R, Horie A, Hashimoto Y.  Eur J Drug Metab Pharmacokinet  2015  41:819-824  PubMed ID: 26628429
7165  Regulation of Ripply1 expression in MDCK organoids.  Yoshizaki H, Kuwajima Y, Minato H, Kiyokawa E.  Biochem. Biophys. Res. Commun.  2015  468:337-42  PubMed ID: 26514726
7286  Evaluation of the Universal Viral Transport system for long-term storage of virus specimens for microbial forensics.  Hosokawa-Muto J, Fujinami Y, Mizuno N.  J Forensic Leg Med  2015  34:29-33  PubMed ID: 26165655
7485  Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH.  Daidoji T, Watanabe Y, Ibrahim MS, Yasugi M, Maruyama H, Masuda T, Arai F, Ohba T, Honda A, Ikuta K, Nakaya T.  J. Biol. Chem.  2015  290:10627-42  PubMed ID: 25673693
7534  Application of multichannel collagen gels in construction of epithelial lumen-like engineered tissues.  K Furusawa, T Mizutani, H Machino, S Yahata, A Fukui, N Sasaki  ACS Biomater Sci Eng  2015  1:539-548 
7635  MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis.  Hosono Y, Yamaguchi T, Mizutani E, Yanagisawa K, Arima C, Tomida S, Shimada Y, Hiraoka M, Kato S, Yokoi K, Suzuki M, Takahashi T.  EMBO J.  2011  31:481-93  PubMed ID: 22085929
7743  Cellular vacuolation induced by Clostridium perfringens epsilon-toxin.  Nagahama M, Itohayashi Y, Hara H, Higashihara M, Fukatani Y, Takagishi T, Oda M, Kobayashi K, Nakagawa I, Sakurai J.  FEBS J.  2011  278:3395-407  PubMed ID: 21781280
7798  Acquisition of human-type receptor binding specificity by new H5N1 influenza virus sublineages during their emergence in birds in Egypt.  Watanabe Y, Ibrahim MS, Ellakany HF, Kawashita N, Mizuike R, Hiramatsu H, Sriwilaijaroen N, Takagi T, Suzuki Y, Ikuta K.  PLoS Pathog.  2011  7:e1002068  PubMed ID: 21637809
7907  A genetically encoded Forster resonance energy transfer biosensor for two-photon excitation microscopy.  Kumagai Y, Kamioka Y, Yagi S, Matsuda M, Kiyokawa E.  Anal. Biochem.  2011  413:192-9  PubMed ID: 21352796
8048  Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice.  Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N, Suzuki T.  Int. Immunopharmacol.  2011  11:199-203  PubMed ID: 21095259
8117  A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface.  Mochida S, Tsuzuki S, Inouye K, Fushiki T.  J. Biochem.  2010  148:721-32  PubMed ID: 20855298
8397  Spatio-temporal PLC activation in parallel with intracellular Ca2+ wave propagation in mechanically stimulated single MDCK cells.  Tsukamoto A, Hayashida Y, Furukawa KS, Ushida T.  Cell Calcium  2010  47:253-63  PubMed ID: 20060585
8479  Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS.  Zhu X, Hatanaka K, Yamagata T, Sato T.  Electrophoresis  2009  30:3519-26  PubMed ID: 19813238
8503  Binding and internalization of Clostridium botulinum C2 toxin.  Nagahama M, Hagiyama T, Kojima T, Aoyanagi K, Takahashi C, Oda M, Sakaguchi Y, Oguma K, Sakurai J.  Infect. Immun.  2009  77:5139-48  PubMed ID: 19720757
8710  Displacement of tight junction proteins from detergent-resistant membrane domains by treatment with sodium caprate.  Sugibayashi K, Onuki Y, Takayama K.  Eur J Pharm Sci  2008  36:246-53  PubMed ID: 19013238
8721  Cellular aggregation facilitates anoikis in MDCK cells.  Emoto Y.  J Physiol Sci  2008  58:371-80  PubMed ID: 18842164
3219  Binding and internalization of Clostridium perfringens iota-toxin in lipid rafts.  Nagahama M, Yamaguchi A, Hagiyama T, Ohkubo N, Kobayashi K, Sakurai J.  Infect. Immun.  2004  72:3267-75  PubMed ID: 15155629
9986  Cell Density-Dependent Increase in Tyrosine-Monophosphorylated ERK2 in MDCK Cells Expressing Active Ras or Raf.  Kawabata N, Matsuda M.  PLoS ONE  2016  11:e0167940  PubMed ID: 27936234
10515  In vitro tubulogenesis of Madin-Darby canine kidney (MDCK) spheroids occurs depending on constituent cell number and scaffold gel concentration.  Hirashima T, Hoshuyama M, Adachi T.  J. Theor. Biol.  2017  435:110-115  PubMed ID: 28923372
10604  Propagating Wave of ERK Activation Orients Collective Cell Migration.  Aoki K, Kondo Y, Naoki H, Hiratsuka T, Itoh RE, Matsuda M.  Dev. Cell  2017  43:305-317.e5  PubMed ID: 29112851
10717  Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy.  Zhou Y, Saito M, Miyamoto T, Novak P, Shevchuk AI, Korchev YE, Fukuma T, Takahashi Y.  Anal. Chem.  2018  90:2891-2895  PubMed ID: 29345134
10801  Photoactivatable substrates for systematic study of the impact of an extracellular matrix ligand on appearance of leader cells in collective cell migration.  Abdellatef SA, Nakanishi J.  Biomaterials  2018  169:72-84  PubMed ID: 29655082
10863  Development of the evaluation system for barrier functions of engineered epithelial lumens    Regenerative Therapy  2016  3, 82-89 
11030  Upregulation of multiple signaling pathways by Dock5 deletion in epithelial cells  Xu X, Yoshizaki H, Ishigaki Y, Kubo E, Minato H, Kiyokawa E.  Mol Vis  2017  23:1081-1092  PubMed ID: 29872253
11055  Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a LightResponsive Polymer  Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K.  Commun Biol  2018  1:218  PubMed ID: 30534610
11225  Canine squamous cell carcinoma cell lines with high expression of survivin are sensitive to survivin inhibitor YM155.  Miyamoto R, Kurita S, Tani H, Ikeda T, Ishizaka M, Saima H, Kobayashi M, Tamura K, Bonkobara M.  Vet. J.  2018    PubMed ID: 30268330
11494  Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport.  Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N.  J. Cell Biol.  2018  217(12):4164-4183  PubMed ID: 30297389
11238  Interaction of Clostridium perfringens Iota Toxin and Lipolysis-Stimulated Lipoprotein Receptor (LSR).  Nagahama M, Takehara M, Kobayashi K.  Toxins (Basel)  2018    PubMed ID: 30297616
11278  An Application of Photoactivatable Substrate for the Evaluation of Epithelial-mesenchymal Transition Inhibitors.  Nakanishi J, Sugiyama K, Matsuo H, Takahashi Y, Omura S, Nakashima T.  Anal Sci  2019    PubMed ID: 30393243
11315  Genetic Compatibility of Reassortants between Avian H5N1 and H9N2 Influenza Viruses with Higher Pathogenicity in Mammals.  Arai Y, Ibrahim MS, Elgendy EM, Daidoji T, Ono T, Suzuki Y, Nakaya T, Matsumoto K, Watanabe Y.  J. Virol.  2019    PubMed ID: 30463961
11362  Induced Proton Perturbation for Sensitive and Selective Detection of Tight Junction Breakdown.  Hatano H, Goda T, Matsumoto A, Miyahara Y.  Anal. Chem.  2019    PubMed ID: 30596415
4121  N-terminal dual lipidation-coupled molecular targeting into the primary cilium.  Kumeta M, Panina Y, Yamazaki H, Takeyasu K, Yoshimura SH.  Genes Cells  2018    PubMed ID: 29900630
4289  Acid Sphingomyelinase Promotes Cellular Internalization of Clostridium perfringens Iota-Toxin.  Nagahama M, Takehara M, Miyamoto K, Ishidoh K, Kobayashi K.  Toxins (Basel)  2018    PubMed ID: 29783772
4290  A Novel Morphological Marker for the Analysis of Molecular Activities at the Single-cell Level.  Imanishi A, Murata T, Sato M, Hotta K, Imayoshi I, Matsuda M, Terai K.  Cell Struct. Funct.  2018    PubMed ID: 29962383
4465  Characterization of H5N1 Influenza Virus Quasispecies with Adaptive Hemagglutinin Mutations from Single-Virus Infections of Human Airway Cells.  Watanabe Y, Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Kajikawa J, Hiramatsu H, Sriwilaijaroen N, Ono T, Takagi T, Takahashi K, Shioda T, Matsumoto K, Suzuki Y, Nakaya T.  J. Virol.  2018  92(11)  PubMed ID: 29563293
5331  FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics.  Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata S, Matsuda M.  Nat Methods  2019  16(10):1029-1036  PubMed ID: 31501546
5332  Activated Ras protein accelerates cell cycle progression to perturb Madin-Darby canine kidney cystogenesis.  Sakurai A, Matsuda M, Kiyokawa E.  J Biol Chem  2012  287(38):31703-11  PubMed ID: 22829590
5335  Chimaerin suppresses Rac1 activation at the apical membrane to maintain the cyst structure.  Yagi S, Matsuda M, Kiyokawa E.  PLoS One  2012  7(12):e52258  PubMed ID: 23284959
5336  Comparative lipid analysis in the normal and cancerous organoids of MDCK cells.  Yoshizaki H, Ogiso H, Okazaki T, Kiyokawa E.  J Biochem  2016  159(6):573-84  PubMed ID: 26783265
5338  Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothionein expression.  Sogawa N, Hirai K, Sogawa C, Ohyama K, Miyazaki I, Tsukamoto G, Asanuma M, Sasaki A, Kitayama S.  Life Sci  2013  92(12):727-32  PubMed ID: 2339970
5339  Reduced adhesive ligand density in engineered extracellular matrices induces an epithelial-mesenchymal-like transition.  Marlar S, Abdellatef SA, Nakanishi J.  Acta Biomater  2016  39:106-113  PubMed ID: 27163400
5340  Insufficient ability of Rac1b to perturb cystogenesis.  Mori Y, Yagi S, Sakurai A, Matsuda M, Kiyokawa E.  Small GTPases  2013  4(1):9-15  PubMed ID: 23411476
5342  Small molecular mass inhibitor of growth of MDCK cells derived from pig spinal cord.  Emoto Y, Kawahara E, Koga Y, Ito T, Tawada K.  J Cell Physiol  2001  186(3):350-6  PubMed ID: 11169973
5343  Dynamic behaviors of vimentin induced by interaction with GlcNAc molecules.  Komura K, Ise H, Akaike T.  Glycobiology  2012  22(12):1741-59  PubMed ID: 22846177
5344  Elasticity and Topography-Controlled Collagen Hydrogels Mimicking Native Cellular Milieus  Tomoko Gowa Oyama, View ORCID ProfileKotaro Oyama, Atsushi Kimura, Fumiya Yoshida, Ryo Ishida, Masashi Yamazaki, Hiromi Miyoshi, View ORCID ProfileMitsumasa Taguchi  bioRxiv  2019   
5436  Situations Leading to Reduced Effectiveness of Current Hand Hygiene against Infectious Mucus from Influenza Virus-Infected Patients.  Hirose R, Nakaya T, Naito Y, Daidoji T, Bandou R, Inoue K, Dohi O, Yoshida N, Konishi H, Itoh Y.  mSphere  2019  4(5)  PubMed ID: 31533996
3765  Tyrosine phosphorylation and cellular redistribution of ezrin in MDCK cells treated with pervanadate.  Wu YX, Uezato T, Fujita M.  J Cell Biochem  2000  79(2):311-21  PubMed ID: 10967558
5776  Photoresponsive Aqueous Dissolution of Poly( N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition.  Sumaru K, Takagi T, Morishita K, Kanamori T.  Biomacromolecules  2018  19(7):2913-2922  PubMed ID: 29641886
11621  PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.  Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Ono T, Takagi T, Nakaya T, Matsumoto K, Watanabe Y.  PLoS Pathog.  2019    PubMed ID: 31265471
11826  Nanaomycin K, a new epithelial-mesenchymal transition inhibitor produced by the actinomycete "Streptomyces rosa subsp. notoensis" OS-3966.  Matsuo H, Nakanishi J, Noguchi Y, Kitagawa K, Shigemura K, Sunazuka T, Takahashi Y, Ōmura S, Nakashima T.  J. Biosci. Bioeng.  2019    PubMed ID: 31582334



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