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RCB0995 : MDCK  update : 2018/07/31
CommentCanine kidney epithelial. Sensitive to wide range of viruses. Backup of ECA84121903 .
Comment from the depositor
Use conditiona
Use condition descriptionBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Originator Madin, S. H. & Darby, N. B.
Year of deposit 1994
Animal dog < Mammals
Genus Canis
Species familiaris
Tissue kidney
Classification other
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 31

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Madin, S.H. and Darby, N.B.    Proc Soc Exp Biol Med  1958  98:574 
Oberleithner H, Vogel U, Kersting U, Steigner W  Madin-Darby canine kidney cells. II. Aldosterone stimulates Na+/H+ and Cl-/HCO3- exchange.  Pflugers Arch  1990  416:533-9 

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User's Publication
Fujisawa H  Inhibitory role of neutrophils on influenza virus multiplication in the lungs of mice.  Microbiol Immunol  2001  45:679-88 
Yazawa S, Okada M, Ono M, Fujii S, Okuda Y, Shibata I, Kida H  Experimental dual infection of pigs with an H1N1 swine influenza virus (A/Sw/Hok/2/81) and Mycoplasma hyopneumoniae.  Vet Microbiol  2004  98:221-8 
Sugimoto K, Fukuda R, Himeno M  Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein.  Cell Struct Funct  2000  25:253-61 
Nagahama, Masahiro, Yamaguchi, Akiwo, Hagiyama, Tohko, Ohkubo, Noriko, Kobayashi, Keiko, Sakurai, Jun  Binding and internalization of Clostridium perfringens iota-toxin in lipid rafts  Infect Med  2004  72:3267-3275  PubMed ID: 15155629
Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.  Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss.  EMBO Mol Med  2016  8:1310-1324  PubMed ID: 27707755
Fukao M, Kondo E, Nishino H, Hattori R, Horie A, Hashimoto Y.  Presence of an H(+)/Quinidine Antiport System in Madin-Darby Canine Kidney Cells.  Eur J Drug Metab Pharmacokinet  2015  41:819-824  PubMed ID: 26628429
Yoshizaki H, Kuwajima Y, Minato H, Kiyokawa E.  Regulation of Ripply1 expression in MDCK organoids.  Biochem. Biophys. Res. Commun.  2015  468:337-42  PubMed ID: 26514726
Hosokawa-Muto J, Fujinami Y, Mizuno N.  Evaluation of the Universal Viral Transport system for long-term storage of virus specimens for microbial forensics.  J Forensic Leg Med  2015  34:29-33  PubMed ID: 26165655
Daidoji T, Watanabe Y, Ibrahim MS, Yasugi M, Maruyama H, Masuda T, Arai F, Ohba T, Honda A, Ikuta K, Nakaya T.  Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH.  J. Biol. Chem.  2015  290:10627-42  PubMed ID: 25673693
K Furusawa, T Mizutani, H Machino, S Yahata, A Fukui, N Sasaki  Application of multichannel collagen gels in construction of epithelial lumen-like engineered tissues.  ACS Biomater Sci Eng  2015  1:539-548 
Hosono Y, Yamaguchi T, Mizutani E, Yanagisawa K, Arima C, Tomida S, Shimada Y, Hiraoka M, Kato S, Yokoi K, Suzuki M, Takahashi T.  MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis.  EMBO J.  2011  31:481-93  PubMed ID: 22085929
Nagahama M, Itohayashi Y, Hara H, Higashihara M, Fukatani Y, Takagishi T, Oda M, Kobayashi K, Nakagawa I, Sakurai J.  Cellular vacuolation induced by Clostridium perfringens epsilon-toxin.  FEBS J.  2011  278:3395-407  PubMed ID: 21781280
Watanabe Y, Ibrahim MS, Ellakany HF, Kawashita N, Mizuike R, Hiramatsu H, Sriwilaijaroen N, Takagi T, Suzuki Y, Ikuta K.  Acquisition of human-type receptor binding specificity by new H5N1 influenza virus sublineages during their emergence in birds in Egypt.  PLoS Pathog.  2011  7:e1002068  PubMed ID: 21637809
Kumagai Y, Kamioka Y, Yagi S, Matsuda M, Kiyokawa E.  A genetically encoded Forster resonance energy transfer biosensor for two-photon excitation microscopy.  Anal. Biochem.  2011  413:192-9  PubMed ID: 21352796
Mochida S, Tsuzuki S, Inouye K, Fushiki T.  A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface.  J. Biochem.  2010  148:721-32  PubMed ID: 20855298
Tsukamoto A, Hayashida Y, Furukawa KS, Ushida T.  Spatio-temporal PLC activation in parallel with intracellular Ca2+ wave propagation in mechanically stimulated single MDCK cells.  Cell Calcium  2010  47:253-63  PubMed ID: 20060585
Zhu X, Hatanaka K, Yamagata T, Sato T.  Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS.  Electrophoresis  2009  30:3519-26  PubMed ID: 19813238
Nagahama M, Hagiyama T, Kojima T, Aoyanagi K, Takahashi C, Oda M, Sakaguchi Y, Oguma K, Sakurai J.  Binding and internalization of Clostridium botulinum C2 toxin.  Infect. Immun.  2009  77:5139-48  PubMed ID: 19720757
Sugibayashi K, Onuki Y, Takayama K.  Displacement of tight junction proteins from detergent-resistant membrane domains by treatment with sodium caprate.  Eur J Pharm Sci  2008  36:246-53  PubMed ID: 19013238
Emoto Y.  Cellular aggregation facilitates anoikis in MDCK cells.  J Physiol Sci  2008  58:371-80  PubMed ID: 18842164
Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
Nagahama M, Yamaguchi A, Hagiyama T, Ohkubo N, Kobayashi K, Sakurai J.  Binding and internalization of Clostridium perfringens iota-toxin in lipid rafts.  Infect. Immun.  2004  72:3267-75  PubMed ID: 15155629
Kawabata N, Matsuda M.  Cell Density-Dependent Increase in Tyrosine-Monophosphorylated ERK2 in MDCK Cells Expressing Active Ras or Raf.  PLoS ONE  2016  11:e0167940  PubMed ID: 27936234
Hirashima T, Hoshuyama M, Adachi T.  In vitro tubulogenesis of Madin-Darby canine kidney (MDCK) spheroids occurs depending on constituent cell number and scaffold gel concentration.  J. Theor. Biol.  2017  435:110-115  PubMed ID: 28923372
Aoki K, Kondo Y, Naoki H, Hiratsuka T, Itoh RE, Matsuda M.  Propagating Wave of ERK Activation Orients Collective Cell Migration.  Dev. Cell  2017  43:305-317.e5  PubMed ID: 29112851
Zhou Y, Saito M, Miyamoto T, Novak P, Shevchuk AI, Korchev YE, Fukuma T, Takahashi Y.  Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy.  Anal. Chem.  2018  90:2891-2895  PubMed ID: 29345134
Abdellatef SA, Nakanishi J.  Photoactivatable substrates for systematic study of the impact of an extracellular matrix ligand on appearance of leader cells in collective cell migration.  Biomaterials  2018  169:72-84  PubMed ID: 29655082
  Development of the evaluation system for barrier functions of engineered epithelial lumens        PubMed ID:
Xu X, Yoshizaki H, Ishigaki Y, Kubo E, Minato H, Kiyokawa E.  Upregulation of multiple signaling pathways by Dock5 deletion in epithelial cells  Mol Vis  2017  23:1081-1092  PubMed ID: 29872253
Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K.  Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a LightResponsive Polymer  Commun Biol  2018  1:218  PubMed ID: 30534610
Miyamoto R, Kurita S, Tani H, Ikeda T, Ishizaka M, Saima H, Kobayashi M, Tamura K, Bonkobara M.  Canine squamous cell carcinoma cell lines with high expression of survivin are sensitive to survivin inhibitor YM155.  Vet. J.  2018    PubMed ID: 30268330

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