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RCB0993 : IEC 6  update : 2018/07/31
CommentRat small intestine epithelial cells. Backup of ECA88071401 .
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Animal rat < Mammals
Genus Rattus
Species norvegicus
Tissue small intestine
Classification normal
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% FBS + 4μg/ml Insulin
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 23


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Reference
Quaroni A, Wands J, Trelstad, R L, Isselbacher, K J  Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria.  J Cell Biol  1979  80:248-65 

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User's Publication
Uesaka T, Kageyama N, Watanabe H  Identifying target genes regulated downstream of Cdx2 by microarray analysis.  J Mol Biol  2004  337:647-60  PubMed ID: 15019784
Ikenoue, Tsuneo, Hikiba, Yohko, Kanai, Fumihiko, Aragaki, Jun, Tanaka, Yasuo, Imamura, Jun, Imamura, Takaaki, Ohta, Miki, Ijichi, Hideaki, Tateishi, Keisuke, Kawakami, Takayuki, Matsumura, Masayuki, Kawabe, Takao, Omata, Masao  Different effects of point mutations within the B-Raf glycine-rich loop in colorectal tumors on mitogen-activated protein/extracellular signal-regulated kinase kinase/extracellular signal-regulated kinase and nuclear factor kappaB pathway and cellular transformation.  Cancer Res  2004  64:3428-35  PubMed ID: 15150094
Uesaka, Toshihiro, Kageyama, Noriko  Cdx2 homeodomain protein regulates the expression of MOK, a member of the mitogen-activated protein kinase superfamily, in the intestinal epithelial cells.  FEBS Lett  2004  573:147-54  PubMed ID: 15327990
Qian, Wei, Nishikawa, Manabu, Haque, Anwarul Md, Hirose, Masaki, Mashimo, Masayuki, Sato, Eisuke, Inoue, Masayasu  Mitochondrial density determines the cellular sensitivity to cisplatin-induced cell death.  Am J Physiol Cell Physiol  2005  289:C1466-75  PubMed ID: 16107504
Yokoe S, Nakagawa T, Kojima Y, Higuchi K, Asahi M.  Indomethacin-induced intestinal epithelial cell damage is mediated by pVHL activation through the degradation of collagen I and HIF-1α.  Biochem. Biophys. Res. Commun.  2015  468:671-6  PubMed ID: 26551465
Tamaki K, Otaka M, Shibuya T, Sakamoto N, Yamamoto S, Odashima M, Itoh H, Watanabe S.  Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer.  Evid Based Complement Alternat Med  2011  2012:278958  PubMed ID: 21822440
Matsuo T, Miyata K, Inui K, Ito H, Horiuchi R, Suzuki T, Yoneyama T, Oguma K, Niwa K, Watanabe T, Ohyama T.  Characterization of sugar recognition by the toxin complex produced by the Clostridium botulinum serotype C variant strain Yoichi.  FEMS Immunol. Med. Microbiol.  2011  63:35-43  PubMed ID: 21635567
Ishihara H, Tanaka I, Yakumaru H, Tanaka M, Satoh A, Ishiwata A, Yokochi K, Kurematsu A, Ueda J, Shibata T, Hachiya M, Akashi M.  Acceleration of regeneration of mucosa in small intestine damaged by ionizing radiation using anabolic steroids.  Radiat. Res.  2011  175:367-74  PubMed ID: 21388280
Tamaki K, Otaka M, Takada M, Yamamoto S, Odashima M, Itoh H, Watanabe S.  Evidence for enhanced cytoprotective function of HSP90-overexpressing small intestinal epithelial cells.  Dig. Dis. Sci.  2011  56:1954-61  PubMed ID: 21253835
Ito H, Sagane Y, Miyata K, Inui K, Matsuo T, Horiuchi R, Ikeda T, Suzuki T, Hasegawa K, Kouguchi H, Oguma K, Niwa K, Ohyama T, Watanabe T.  HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer.  FEMS Immunol. Med. Microbiol.  2011  61:323-31  PubMed ID: 21219447
Takeda K, Hashimoto K, Uchikawa R, Tegoshi T, Yamada M, Arizono N.  Direct effects of IL-4/IL-13 and the nematode Nippostrongylus brasiliensis on intestinal epithelial cells in vitro.  Parasite Immunol.  2010  32:420-9  PubMed ID: 20500673
Sato N, Morimoto H, Baba R, Nakamata J, Doi Y, Yamaguchi K.  Functional expression of double-stranded RNA-dependent protein kinase in rat intestinal epithelial cells.  J. Cell. Biochem.  2010  110:104-11  PubMed ID: 20213745
Takada M, Otaka M, Takahashi T, Izumi Y, Tamaki K, Shibuya T, Sakamoto N, Osada T, Yamamoto S, Ishida R, Odashima M, Itoh H, Watanabe S.  Overexpression of a 60-kDa heat shock protein enhances cytoprotective function of small intestinal epithelial cells.  Life Sci.  2010  86:499-504  PubMed ID: 20159025
Niwa K, Yoneyama T, Ito H, Taira M, Chikai T, Kouguchi H, Suzuki T, Hasegawa K, Miyata K, Inui K, Ikeda T, Watanabe T, Ohyama T.  Sialic acid-dependent binding and transcytosis of serotype D botulinum neurotoxin and toxin complex in rat intestinal epithelial cells.  Vet. Microbiol.  2009  141:312-20  PubMed ID: 19786330
Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
Miyashita S, Sagane Y, Suzuki T, Matsumoto T, Niwa K, Watanabe T.  Non-Toxic Proteins of the Botulinum Toxin Complex Exert In-vivo Toxicity.  Sci Rep  2016  6:31043  PubMed ID: 27507612
Kojo Y, Matsunaga S, Suzuki H, Sato H, Seto Y, Onoue S.  Improved oral absorption profile of itraconazole in hypochlorhydria by self-micellizing solid dispersion approach.  Eur J Pharm Sci  2017  97:55-61  PubMed ID: 27810562
Tsukahara T, Hamouda N, Utsumi D, Matsumoto K, Amagase K, Kato S.  G protein-coupled receptor 35 contributes to mucosal repair in mice via migration of colonic epithelial cells.  Pharmacol. Res.  2017  123:27-39  PubMed ID: 28648739
Kamao M, Hirota Y, Suhara Y, Tsugawa N, Nakagawa K, Okano T, Hasegawa H.  Determination of Menadione by Liquid Chromatography-Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring.  Anal Sci  2017  33:863-867  PubMed ID: 28690267
Mashima H, Watanabe N, Sekine M, Matsumoto S, Asano T, Yuhashi K, Sagihara N, Urayoshi S, Uehara T, Fujiwara J, Ishii T, Tsuboi R, Miyatani H, Ohnishi H.  The role of Gαq/Gα11 signaling in intestinal epithelial cells.  Biochem Biophys Rep  2018  13:93-98  PubMed ID: 29387814
Islam MS, Kaji N, Mikawa S, Yang Q, Kusabe M, Hori M, Ozaki H.  Induction of myosin light chain kinase and CPI-17 by TGF-β accelerates contractile activity in intestinal epithelial cells.  J. Vet. Med. Sci.  2018    PubMed ID: 29695674
  Interleukin-33, an interleukin-1 like cytokine accelerates cell migration in IEC-6 cells  Asian Journal of Medical and Biological Research  2016   2 
Matsui T, Ichikawa H, Fujita T, Takemura S, Takagi T, Osada-Oka M, Minamiyama Y.  Histidine and arginine modulate intestinal cell restitution via transforming growth factor-β1.  Eur. J. Pharmacol.  2019    PubMed ID: 30753862



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