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RCB0993 : IEC 6  update : 2019/12/11
CommentRat small intestine epithelial cells. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88071401 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88071401 prior to order.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Animal rat < Mammals
Genus Rattus
Species norvegicus
Tissue small intestine
Classification normal
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 5% FBS + 4μg/ml Insulin
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 27

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1831  Quaroni A, Wands J, Trelstad, R L, Isselbacher, K J  Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria.  J Cell Biol  1979  80:248-65  PubMed ID: 88453

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User's Publication
2907  Uesaka T, Kageyama N, Watanabe H  Identifying target genes regulated downstream of Cdx2 by microarray analysis.  J Mol Biol  2004  337:647-60  PubMed ID: 15019784
2864  Ikenoue, Tsuneo, Hikiba, Yohko, Kanai, Fumihiko, Aragaki, Jun, Tanaka, Yasuo, Imamura, Jun, Imamura, Takaaki, Ohta, Miki, Ijichi, Hideaki, Tateishi, Keisuke, Kawakami, Takayuki, Matsumura, Masayuki, Kawabe, Takao, Omata, Masao  Different effects of point mutations within the B-Raf glycine-rich loop in colorectal tumors on mitogen-activated protein/extracellular signal-regulated kinase kinase/extracellular signal-regulated kinase and nuclear factor kappaB pathway and cellular transformation.  Cancer Res  2004  64:3428-35  PubMed ID: 15150094
3178  Uesaka, Toshihiro, Kageyama, Noriko  Cdx2 homeodomain protein regulates the expression of MOK, a member of the mitogen-activated protein kinase superfamily, in the intestinal epithelial cells.  FEBS Lett  2004  573:147-54  PubMed ID: 15327990
3408  Qian, Wei, Nishikawa, Manabu, Haque, Anwarul Md, Hirose, Masaki, Mashimo, Masayuki, Sato, Eisuke, Inoue, Masayasu  Mitochondrial density determines the cellular sensitivity to cisplatin-induced cell death.  Am J Physiol Cell Physiol  2005  289:C1466-75  PubMed ID: 16107504
7156  Yokoe S, Nakagawa T, Kojima Y, Higuchi K, Asahi M.  Indomethacin-induced intestinal epithelial cell damage is mediated by pVHL activation through the degradation of collagen I and HIF-1α.  Biochem. Biophys. Res. Commun.  2015  468:671-6  PubMed ID: 26551465
7726  Tamaki K, Otaka M, Shibuya T, Sakamoto N, Yamamoto S, Odashima M, Itoh H, Watanabe S.  Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer.  Evid Based Complement Alternat Med  2011  2012:278958  PubMed ID: 21822440
7796  Matsuo T, Miyata K, Inui K, Ito H, Horiuchi R, Suzuki T, Yoneyama T, Oguma K, Niwa K, Watanabe T, Ohyama T.  Characterization of sugar recognition by the toxin complex produced by the Clostridium botulinum serotype C variant strain Yoichi.  FEMS Immunol. Med. Microbiol.  2011  63:35-43  PubMed ID: 21635567
7890  Ishihara H, Tanaka I, Yakumaru H, Tanaka M, Satoh A, Ishiwata A, Yokochi K, Kurematsu A, Ueda J, Shibata T, Hachiya M, Akashi M.  Acceleration of regeneration of mucosa in small intestine damaged by ionizing radiation using anabolic steroids.  Radiat. Res.  2011  175:367-74  PubMed ID: 21388280
7981  Tamaki K, Otaka M, Takada M, Yamamoto S, Odashima M, Itoh H, Watanabe S.  Evidence for enhanced cytoprotective function of HSP90-overexpressing small intestinal epithelial cells.  Dig. Dis. Sci.  2011  56:1954-61  PubMed ID: 21253835
7998  Ito H, Sagane Y, Miyata K, Inui K, Matsuo T, Horiuchi R, Ikeda T, Suzuki T, Hasegawa K, Kouguchi H, Oguma K, Niwa K, Ohyama T, Watanabe T.  HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer.  FEMS Immunol. Med. Microbiol.  2011  61:323-31  PubMed ID: 21219447
8245  Takeda K, Hashimoto K, Uchikawa R, Tegoshi T, Yamada M, Arizono N.  Direct effects of IL-4/IL-13 and the nematode Nippostrongylus brasiliensis on intestinal epithelial cells in vitro.  Parasite Immunol.  2010  32:420-9  PubMed ID: 20500673
8339  Sato N, Morimoto H, Baba R, Nakamata J, Doi Y, Yamaguchi K.  Functional expression of double-stranded RNA-dependent protein kinase in rat intestinal epithelial cells.  J. Cell. Biochem.  2010  110:104-11  PubMed ID: 20213745
8361  Takada M, Otaka M, Takahashi T, Izumi Y, Tamaki K, Shibuya T, Sakamoto N, Osada T, Yamamoto S, Ishida R, Odashima M, Itoh H, Watanabe S.  Overexpression of a 60-kDa heat shock protein enhances cytoprotective function of small intestinal epithelial cells.  Life Sci.  2010  86:499-504  PubMed ID: 20159025
8485  Niwa K, Yoneyama T, Ito H, Taira M, Chikai T, Kouguchi H, Suzuki T, Hasegawa K, Miyata K, Inui K, Ikeda T, Watanabe T, Ohyama T.  Sialic acid-dependent binding and transcytosis of serotype D botulinum neurotoxin and toxin complex in rat intestinal epithelial cells.  Vet. Microbiol.  2009  141:312-20  PubMed ID: 19786330
9781  Miyashita S, Sagane Y, Suzuki T, Matsumoto T, Niwa K, Watanabe T.  Non-Toxic Proteins of the Botulinum Toxin Complex Exert In-vivo Toxicity.  Sci Rep  2016  6:31043  PubMed ID: 27507612
9907  Kojo Y, Matsunaga S, Suzuki H, Sato H, Seto Y, Onoue S.  Improved oral absorption profile of itraconazole in hypochlorhydria by self-micellizing solid dispersion approach.  Eur J Pharm Sci  2017  97:55-61  PubMed ID: 27810562
10382  Tsukahara T, Hamouda N, Utsumi D, Matsumoto K, Amagase K, Kato S.  G protein-coupled receptor 35 contributes to mucosal repair in mice via migration of colonic epithelial cells.  Pharmacol. Res.  2017  123:27-39  PubMed ID: 28648739
10414  Kamao M, Hirota Y, Suhara Y, Tsugawa N, Nakagawa K, Okano T, Hasegawa H.  Determination of Menadione by Liquid Chromatography-Tandem Mass Spectrometry Using Pseudo Multiple Reaction Monitoring.  Anal Sci  2017  33:863-867  PubMed ID: 28690267
10726  Mashima H, Watanabe N, Sekine M, Matsumoto S, Asano T, Yuhashi K, Sagihara N, Urayoshi S, Uehara T, Fujiwara J, Ishii T, Tsuboi R, Miyatani H, Ohnishi H.  The role of Gαq/Gα11 signaling in intestinal epithelial cells.  Biochem Biophys Rep  2018  13:93-98  PubMed ID: 29387814
10825  Islam MS, Kaji N, Mikawa S, Yang Q, Kusabe M, Hori M, Ozaki H.  Induction of myosin light chain kinase and CPI-17 by TGF-β accelerates contractile activity in intestinal epithelial cells.  J. Vet. Med. Sci.  2018    PubMed ID: 29695674
10931    Interleukin-33, an interleukin-1 like cytokine accelerates cell migration in IEC-6 cells  Asian Journal of Medical and Biological Research  2016  2 
11431  Matsui T, Ichikawa H, Fujita T, Takemura S, Takagi T, Osada-Oka M, Minamiyama Y.  Histidine and arginine modulate intestinal cell restitution via transforming growth factor-β1.  Eur. J. Pharmacol.  2019    PubMed ID: 30753862
3789  Uesaka T, Lu H, Katoh O, Watanabe H.  Heparin-binding EGF-like growth factor gene transcription regulated by Cdx2 in the intestinal epithelium.  Am J Physiol Gastrointest Liver Physiol  2002  283(4):G840-7  PubMed ID: 12223343
3877  Yuki T, Ishihara S, Rumi MA, Ortega-Cava CF, Kadowaki Y, Kazumori H, Ishimura N, Amano Y, Moriyama N, Kinoshita Y.  Increased expression of midkine in the rat colon during healing of experimental colitis.  Am J Physiol Gastrointest Liver Physiol  2006  291(4):G735-43  PubMed ID: 16959957
3894  Nagano Y1, Matsui H, Shimokawa O, Hirayama A, Tamura M, Nakamura Y, Kaneko T, Rai K, Indo HP, Majima HJ, Hyodo I.  Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cells.  J Physiol Pharmacol  2012  63(2):137-42  PubMed ID: 22653900
3909  Nagano Y, Matsui H, Shimokawa O, Hirayama A, Nakamura Y, Tamura M, Rai K, Kaneko T, Hyodo I.  Bisphosphonate-induced gastrointestinal mucosal injury is mediated by mitochondrial superoxide production and lipid peroxidation.  J Clin Biochem Nutr  2012  51(3):196-203  PubMed ID: 23170047
3919  Tatsunobu MatsuiM.S. et al  Histidine deficiency attenuates cell viability in rat intestinal epithelial cells by apoptosis via mitochondrial dysfunction  Journal of Nutrition & Intermediary Metabolism  2017  8:21-28 

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