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RCB0992 : HOS  update : 2019/12/11
CommentSensitive to further transformation with both virus and chemicals. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA87070202 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA87070202 prior to order.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Another name TE 85, clone F-5
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 13 years
Tissue bone
Case history Osteogenic sarcoma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 47-76(50) : 47(5),48(15),49(20),50(9),76(1)
deposit info
lot info
Reference information Reference 3
User's Publication 13

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1827  Rhim, J S, Cho, H Y, Huebner, R J  Non-producer human cells induced by murine sarcoma virus.  Int J Cancer  1975  15:23-9  PubMed ID: 165148
1828  Rhim, J S, Cho, H Y, Vernon, M L, Arnstein P, Huebner, R J, Gilden, R V  Characterization of non-producer human cells induced by Kirsten sarcoma virus.  Int J Cancer  1975  16:840-9  PubMed ID: 171229
1829  McAllister, R M, Gardner, M B, Greene, A E, Bradt C, Nichols, W W, Landing, B H  Cultivation in vitro of cells derived from a human osteosarcoma.  Cancer  1971  27:397-402  PubMed ID: 5100401

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User's Publication
10773  Yamada N, Kato-Kogoe N, Yamanegi K, Nishiura H, Fujihara Y, Fukunishi S, Okamura H, Terada N, Nakasho K.  Inhibition of Asparagine-linked Glycosylation Participates in Hypoxia-induced Down-regulation of Cell-surface MICA Expression.  Anticancer Res.  2018  38:1353-1359  PubMed ID: 29491059
4234  Ito T, Ando T, Suzuki-Karasaki M, Tokunaga T, Yoshida Y, Ochiai T, Tokuhashi Y, Suzuki-Karasaki Y.  Cold PSM, but not TRAIL, triggers autophagic cell death: A therapeutic advantage of PSM over TRAIL.  Int. J. Oncol.  2018    PubMed ID: 29845256
9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427
10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760 
7838  Tsunashima Y, Kondo A, Matsuda T, Togari A.  Hydrocortisone inhibits cellular proliferation by downregulating hepatocyte growth factor synthesis in human osteoblasts.  Biol. Pharm. Bull.  2011  34:700-3  PubMed ID: 21532160
8537  Shinji Iizuka, Yasusei Kudo, Maki Yoshida, Takaaki Tsunematsu, Yuji Yoshiko, Takashi Uchida, Ikuko Ogawa, Mutsumi Miyauchi, and Takashi Takata  Ameloblastin Regulates Osteogenic Differentiation by Inhibiting Src Kinase via Cross Talk between Integrin 1 and CD63  MOLECULAR AND CELLULAR BIOLOGY  2011  31:783–792 
8031  Iizuka S, Kudo Y, Yoshida M, Tsunematsu T, Yoshiko Y, Uchida T, Ogawa I, Miyauchi M, Takata T.  Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.  Mol. Cell. Biol.  2010  31:783-92  PubMed ID: 21149578
8056  Hori T, Kondo T, Lee H, Song CW, Park HJ.  Hyperthermia enhances the effect of β-lapachone to cause γH2AX formations and cell death in human osteosarcoma cells.  Int J Hyperthermia  2010  27:53-62  PubMed ID: 21070139
8261  Matsuda T, Kondo A, Tsunashima Y, Togari A.  Inhibitory effect of vitamin K(2) on interleukin-1beta-stimulated proliferation of human osteoblasts.  Biol. Pharm. Bull.  2010  33:804-8  PubMed ID: 20460758
8542  Hori T, Kondo T, Kanamori M, Tabuchi Y, Ogawa R, Zhao QL, Ahmed K, Yasuda T, Seki S, Suzuki K, Kimura T.  Nutlin-3 enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through up-regulation of death receptor 5 (DR5) in human sarcoma HOS cells and human colon cancer HCT116 cells.  Cancer Lett.  2009  287:98-108  PubMed ID: 19577358
3041  Terasaki, Tadashi, Kyo, Satoru, Takakura, Masahiro, Maida, Yoshiko, Tsuchiya, Hiroyuki, Tomita, Katsuro, Inoue, Masaki  Analysis of telomerase activity and telomere length in bone and soft tissue tumors.  Oncol Rep  2004  11:1307-11  PubMed ID: 15138570
3962  Yoshiko Y, Son A, Maeda S, Igarashi A, Takano S, Hu J, Maeda N.  Evidence for stanniocalcin gene expression in mammalian bone.  Endocrinology  1999  140(4):1869-74  PubMed ID: 10098526
3970  Kashima T, Kawaguchi J, Takeshita S, Kuroda M, Takanashi M, Horiuchi H, Imamura T, Ishikawa Y, Ishida T, Mori S, Machinami R, Kudo A.  Anomalous cadherin expression in osteosarcoma. Possible relationships to metastasis and morphogenesis.  Am J Pathol  1999  155(5):1549-55  PubMed ID: 10550312

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