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RCB0991 : G-361  update : 2019/12/11
CommentHuman melanoma producing melanin up to 50 PDL. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88030401 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA88030401 prior to order.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 31 years
Tissue skin
Case history malignant melanoma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 65-133(50) : /67(3),68(5),69(13),70(7),71(6),72(4)/
deposit info
lot info
Reference information Reference 1
User's Publication 4

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125  Paul T Peebles, Timothy Trisch & Alex G Papageorge   727 ISOLATION OF FOUR UNUSUAL PEDIATRIC SOLID TUMOR CELL LINES  Pediatric Research  1978  12:485 

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User's Publication
11645  Yamada H, Hakozaki M, Uemura A, Yamashita T.  Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.  J. Lipid Res.  2019    PubMed ID: 31345992
4187  Fujiwara S, Nagai H, Jimbo H, Jimbo N, Tanaka T, Inoie M, Nishigori C.  Gene Expression and Methylation Analysis in Melanomas and Melanocytes From the Same Patient: Loss of NPM2 Expression Is a Potential Immunohistochemical Marker for Melanoma.  Front Oncol  2018    PubMed ID: 30719424
7610  Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.  A novel hairless mouse model for malignant melanoma.  J. Dermatol. Sci.  2011  65:207-12  PubMed ID: 22169325
9063  Daisuke Watanabe, Fumi Goshima, Isamu Mori, Yasuhiko Tamada, Yoshinari Matsumoto, Yukihiro Nishiyama  Oncolytic Virotherapy for Malignant Melanoma With Herpes Simplex Virus Type 1 Mutant HF10  J Dermatol Sci  2008  50 (3), 185-96  PubMed ID: 18226503

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