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RCB0991 : G-361  update : 2018/07/31
CommentHuman melanoma producing melanin up to 50 PDL. Backup of ECA88030401 .
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 31 years
Tissue skin
Case history malignant melanoma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 65-133(50) : /67(3),68(5),69(13),70(7),71(6),72(4)/
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
    Pediatr Res  1978  0.836805556 

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User's Publication
Thang ND, Yajima I, Nakagawa K, Tsuzuki T, Kumasaka MY, Ohgami N, Ly TB, Iwamoto T, Watanabe D, Kato M.  A novel hairless mouse model for malignant melanoma.  J. Dermatol. Sci.  2011  65:207-12  PubMed ID: 22169325



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