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RCB0987 : C2C12  update : 2018/07/31
CommentSaid differentiate to myotubes, but need confirmation. Backup of ECA91031101 .
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 1994
Animal mouse < Mammals
Strain name C3H
Tissue muscle
Classification other
History overseas, ECACC
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 121


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Reference
Yaffe D, Saxel O  Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.  Nature  1977  270:725-7 

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User's Publication
Funato N, Ohyama K, Kuroda T, Nakamura M  Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription  J Biol Chem  2002  278:7486-7493  PubMed ID: 12493738
Yang XL, Matsuura H, Fu Y, Sugiyama T, Miura N  MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts.  FEBS Lett  2000  470:29-34 
Notoya M, Otsuka E, Yamaguchi A, Hagiwara H  Runx-2 is not essential for the vitamin D-regulated expression of RANKL and osteoprotegerin in osteoblastic cells.  Biochem Biophys Res Commun  2004  324:655-60  PubMed ID: 15474477
Akimoto T, Ushida T, Miyaki S, Akaogi H, Tsuchiya K, Yan Z, Williams RS, Tateishi T  Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling.  Biochem Biophys Res Commun  2005  329:381-5  PubMed ID: 15721317
Tsutsumi S, Inoue H, Sakamoto Y, Mizuta K, Kamata N, Itakura M  Molecular cloning and characterization of the murine gnathodiaphyseal dysplasia gene GDD1.  Biochem Biophys Res Commun  2005  331:1099-106  PubMed ID: 15882990
Suzuki J, Kaziro Y, Koide H  Positive regulation of skeletal myogenesis by R-Ras.  Oncogene  2000  19:1138-46 
Suzuki J, Yamazaki Y, Li G, Kaziro Y, Koide H  Involvement of Ras and Ral in chemotactic migration of skeletal myoblasts.  Mol Cell Biol  2000  20:4658-65 
Washio K, Kobayashi M, Saito N, Amagasa M, Kitamura H.  Propolis Ethanol Extract Stimulates Cytokine and Chemokine Production through NF-κB Activation in C2C12 Myoblasts.  Evid Based Complement Alternat Med  2015  2015:349751  PubMed ID: 26604971
Toda K, Takeda S, Hitoe S, Nakamura S, Matsuda H, Shimoda H.  Enhancement of energy production by black ginger extract containing polymethoxy flavonoids in myocytes through improving glucose, lactic acid and lipid metabolism.  J Nat Med  2015  70:163-72  PubMed ID: 26581843
Nozaki T, Nikai S, Okabe R, Nagahama K, Eto N.  A novel in vitro model of sarcopenia using BubR1 hypomorphic C2C12 myoblasts.  Cytotechnology  2015  68:1705-15  PubMed ID: 26464273
Oh S, Kato M, Zhang C, Guo Y, Beachy PA.  A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response.  PLoS ONE  2015  10:e0135804  PubMed ID: 26271100
Otani M, Furukawa S, Wakisaka S, Maeda T.  A novel adipokine C1q/TNF-related protein 3 is expressed in developing skeletal muscle and controls myoblast proliferation and differentiation.  Mol. Cell. Biochem.  2015  409:271-82  PubMed ID: 26272338
Ayabe T, Mizushige T, Ota W, Kawabata F, Hayamizu K, Han L, Tsuji T, Kanamoto R, Ohinata K.  A novel Alaska pollack-derived peptide, which increases glucose uptake in skeletal muscle cells, lowers the blood glucose level in diabetic mice.  Food Funct  2015  6:2749-57  PubMed ID: 26152190
Ichimura T, Chiu LD, Fujita K, Machiyama H, Kawata S, Watanabe TM, Fujita H.  Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.  Sci Rep  2015  5:11358  PubMed ID: 26079396
Minami K, Kasuya Y, Yamazaki T, Ji Q, Nakanishi W, Hill JP, Sakai H, Ariga K.  Highly Ordered 1D Fullerene Crystals for Concurrent Control of Macroscopic Cellular Orientation and Differentiation toward Large-Scale Tissue Engineering.  Adv. Mater. Weinheim  2015  27:4020-6  PubMed ID: 26033774
Wu S, Zhang J, Huang C, Jia H, Wang Y, Xu Z, Yang L, Miyagishi M, Kasim V.  Prolyl hydroxylase domain-2 silencing induced by hydrodynamic limb vein injection enhances vascular regeneration in critical limb ischemia mice through activation of multiple genes.  Curr Gene Ther  2015  15:313-25  PubMed ID: 25832622
Takeda I, Kawanabe M, Kaneko A.  Autonomous patterning of cells on microstructured fine particles.  Mater Sci Eng C Mater Biol Appl  2015  50:173-8  PubMed ID: 25746259
Takemoto K, Ishihara S, Mizutani T, Kawabata K, Haga H.  Compressive stress induces dephosphorylation of the myosin regulatory light chain via RhoA phosphorylation by the adenylyl cyclase/protein kinase A signaling pathway.  PLoS ONE  2015  10:e0117937  PubMed ID: 25734240
Takeda T, Tsuiji K, Li B, Tadakawa M, Yaegashi N.  Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells.  Clin Interv Aging  2015  10:445-51  PubMed ID: 25709418
Nakanishi K, Kakiguchi K, Yonemura S, Nakano A, Morishima N.  Transient Ca2+ depletion from the endoplasmic reticulum is critical for skeletal myoblast differentiation.  FASEB J.  2015  29:2137-49  PubMed ID: 25678623
Asano T, Ishizuka T, Morishima K, Yawo H.  Optogenetic induction of contractile ability in immature C2C12 myotubes.  Sci Rep  2015  5:8317  PubMed ID: 25661648
Kaneko K, Higuchi C, Kunugiza Y, Yoshida K, Sakai T, Yoshikawa H, Nakata K.  Hyaluronan inhibits BMP-induced osteoblast differentiation.  FEBS Lett.  2015  589:447-54  PubMed ID: 25592835
Yuki Igaki, Fumio Mizutani, Tomoyuki Yasukawa  Oxygen consumption of contractile C2C12 myotubes investigated by scanning electrochemical microscopy.  Chemistry Letters  2015  44:1031-1032 
Ogawa M, Yamaji R, Higashimura Y, Harada N, Ashida H, Nakano Y, Inui H.  17β-estradiol represses myogenic differentiation by increasing ubiquitin-specific peptidase 19 through estrogen receptor α.  J. Biol. Chem.  2011  286:41455-65  PubMed ID: 21971047
Tanaka S, Terada K, Nohno T.  Canonical Wnt signaling is involved in switching from cell proliferation to myogenic differentiation of mouse myoblast cells.  J Mol Signal  2011  6:12  PubMed ID: 21970630
Suzuki Y, Koshi K, Imai K, Takahashi T, Kizaki K, Hashizume K.  Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines.  Reproduction  2011  142:733-43  PubMed ID: 21862694
Morishima N, Nakanishi K, Nakano A.  Activating transcription factor-6 (ATF6) mediates apoptosis with reduction of myeloid cell leukemia sequence 1 (Mcl-1) protein via induction of WW domain binding protein 1.  J. Biol. Chem.  2011  286:35227-35  PubMed ID: 21841196
Akiyama K, Tone J, Okabe M, Nishimoto S, Sugahara T, Kakinuma Y.  Inhibition of myotube formation by paraquat in the myoblast cell line C2C12.  J Toxicol Sci  2011  36:243-6  PubMed ID: 21467752
Zang L, Nishikawa M, Machida K, Ando M, Takahashi Y, Watanabe Y, Takakura Y.  Inhibition of nuclear delivery of plasmid DNA and transcription by interferon γ: hurdles to be overcome for sustained gene therapy.  Gene Ther.  2011  18:891-7  PubMed ID: 21451577
Itoigawa Y, Kishimoto KN, Sano H, Kaneko K, Itoi E.  Molecular mechanism of fatty degeneration in rotator cuff muscle with tendon rupture.  J. Orthop. Res.  2011  29:861-6  PubMed ID: 21246616
Fujita H, Hirano M, Shimizu K, Nagamori E.  Rapid decrease in active tension generated by C2C12 myotubes after termination of artificial exercise.  J. Muscle Res. Cell. Motil.  2010  31:279-88  PubMed ID: 21120590
Fujita H, Van Dau T, Shimizu K, Hatsuda R, Sugiyama S, Nagamori E.  Designing of a Si-MEMS device with an integrated skeletal muscle cell-based bio-actuator.  Biomed Microdevices  2010  13:123-9  PubMed ID: 20957437
Miyake M, Hayashi S, Iwasaki S, Uchida T, Watanabe K, Ohwada S, Aso H, Yamaguchi T.  TIEG1 negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells.  J. Cell. Physiol.  2010  226:1128-36  PubMed ID: 20945337
Tajika Y, Takahashi M, Hino M, Murakami T, Yorifuji H.  VAMP2 marks quiescent satellite cells and myotubes, but not activated myoblasts.  Acta Histochem Cytochem  2010  43:107-14  PubMed ID: 20824121
Konishi H, Okuda A, Ohno Y, Kihara A.  Characterization of HACD1 K64Q mutant found in arrhythmogenic right ventricular dysplasia patients.  J. Biochem.  2010  148:617-22  PubMed ID: 20724468
Itoigawa Y, Kishimoto KN, Okuno H, Sano H, Kaneko K, Itoi E.  Hypoxia induces adipogenic differentitation of myoblastic cell lines.  Biochem. Biophys. Res. Commun.  2010  399:721-6  PubMed ID: 20692234
Kono S, Nishio T, Takahashi Y, Goto-Inoue N, Kinoshita M, Zaima N, Suzuki H, Fukutoku-Otsuji A, Setou M, Miyajima H.  Dominant-negative effects of a novel mutation in the filamin myopathy.  Neurology  2010  75:547-54  PubMed ID: 20697107
Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Mutou Y, Kobayashi Y, Yorifuji H.  Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells.  J Electron Microsc (Tokyo)  2010  59:495-501  PubMed ID: 20685830
Uemura T, Ohta Y, Nakao Y, Manaka T, Nakamura H, Takaoka K.  Epinephrine accelerates osteoblastic differentiation by enhancing bone morphogenetic protein signaling through a cAMP/protein kinase A signaling pathway.  Bone  2010  47:756-65  PubMed ID: 20637325
Fujita H, Endo A, Shimizu K, Nagamori E.  Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.  Biotechnol. Bioeng.  2010  107:894-901  PubMed ID: 20635352
Fujita H, Shimizu K, Morioka Y, Nagamori E.  Enhancement of C2C12 differentiation by perfluorocarbon-mediated oxygen delivery.  J. Biosci. Bioeng.  2010  110:359-62  PubMed ID: 20547328
Imazato S, Horikawa D, Takeda K, Kiba W, Izutani N, Yoshikawa R, Hayashi M, Ebisu S, Nakano T.  Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials.  Dent Mater J  2010  29:341-6  PubMed ID: 20467157
Yokoi H, Mizukami H, Nagatsu A, Tanabe H, Inoue M.  Hydroxy monounsaturated fatty acids as agonists for peroxisome proliferator-activated receptors.  Biol. Pharm. Bull.  2010  33:854-61  PubMed ID: 20460766
Fujita H, Shimizu K, Nagamori E.  Novel method for measuring active tension generation by C2C12 myotube using UV-crosslinked collagen film.  Biotechnol. Bioeng.  2010  106:482-9  PubMed ID: 20178119
Miyake M, Hayashi S, Iwasaki S, Chao G, Takahashi H, Watanabe K, Ohwada S, Aso H, Yamaguchi T.  Possible role of TIEG1 as a feedback regulator of myostatin and TGF-beta in myoblasts.  Biochem. Biophys. Res. Commun.  2010  393:762-6  PubMed ID: 20171187
Shimizu K, Fujita H, Nagamori E.  Micropatterning of single myotubes on a thermoresponsive culture surface using elastic stencil membranes for single-cell analysis.  J. Biosci. Bioeng.  2010  109:174-8  PubMed ID: 20129103
Shimizu K, Fujita H, Nagamori E.  Oxygen plasma-treated thermoresponsive polymer surfaces for cell sheet engineering.  Biotechnol. Bioeng.  2010  106:303-10  PubMed ID: 20091737
Yamazaki Y, Kamei Y, Sugita S, Akaike F, Kanai S, Miura S, Hirata Y, Troen BR, Kitamura T, Nishino I, Suganami T, Ezaki O, Ogawa Y.  The cathepsin L gene is a direct target of FOXO1 in skeletal muscle.  Biochem. J.  2010  427:171-8  PubMed ID: 20088826
Yuasa K, Masuda T, Yoshikawa C, Nagahama M, Matsuda Y, Tsuji A.  Subtilisin-like proprotein convertase PACE4 is required for skeletal muscle differentiation.  J. Biochem.  2009  146:407-15  PubMed ID: 19520771
Fujita H, Shimizu K, Nagamori E.  Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.  Biotechnol. Bioeng.  2009  103:1034-41  PubMed ID: 19350625
Takayama K, Suzuki A, Manaka T, Taguchi S, Hashimoto Y, Imai Y, Wakitani S, Takaoka K.  RNA interference for noggin enhances the biological activity of bone morphogenetic proteins in vivo and in vitro.  J. Bone Miner. Metab.  2009  27:402-11  PubMed ID: 19252814
Yano K, Hoshino M, Ohta Y, Manaka T, Naka Y, Imai Y, Sebald W, Takaoka K.  Osteoinductive capacity and heat stability of recombinant human bone morphogenetic protein-2 produced by Escherichia coli and dimerized by biochemical processing.  J. Bone Miner. Metab.  2009  27:355-63  PubMed ID: 19229473
Takayuki Hoshino, Tomohiro Konno, Kazuhiko Ishihara, and Keisuke Morishima  Live-Cell-Driven Insertion of a Nanoneedle  Jpn J Appl Phys.  2009  48:107002-1-107002-6 
Takeomi MIZUTANI, Kenichi DOI, Yasuyuki MORITA, Masakazu UCHINO, Mitsugu TODO, Hisashi HAGA and Kazushige KAWABATA  Displacement of Actin Filament and Actin Binding Proteins under Local Deformation Processed by Digital Image Correlation Method in a Myoblast  J Biomechanical Science and Engineering  2009  4:415-422 
Yagi M, Suzuki N, Takayama T, Arisue M, Kodama T, Yoda Y, Otsuka K, Ito K.  Effects of lactoferrin on the differentiation of pluripotent mesenchymal cells.  Cell Biol. Int.  2008  33:283-9  PubMed ID: 19103298
Honda M, Hosoda M, Kanzawa N, Tsuchiya T, Toyo-oka T.  Specific knockdown of delta-sarcoglycan gene in C2C12 in vitro causes post-translational loss of other sarcoglycans without mechanical stress.  Mol. Cell. Biochem.  2008  323:149-59  PubMed ID: 19083155
Yamauchi M, Kawai S, Kato T, Ooshima T, Amano A.  Odd-skipped related 1 gene expression is regulated by Runx2 and Ikzf1 transcription factors.  Gene  2008  426:81-90  PubMed ID: 18804520
Herningtyas EH, Okimura Y, Handayaningsih AE, Yamamoto D, Maki T, Iida K, Takahashi Y, Kaji H, Chihara K.  Branched-chain amino acids and arginine suppress MaFbx/atrogin-1 mRNA expression via mTOR pathway in C2C12 cell line.  Biochim. Biophys. Acta  2008  1780:1115-20  PubMed ID: 18616983
Yamachika E, Tsujigiwa H, Shirasu N, Ueno T, Sakata Y, Fukunaga J, Mizukawa N, Yamada M, Sugahara T.  Immobilized recombinant human bone morphogenetic protein-2 enhances the phosphorylation of receptor-activated Smads.  J Biomed Mater Res A  2008  88:599-607  PubMed ID: 18314893
Takayuki Hoshino, Yuichi Hori, Tomohiro Konno, Kazuhiko Ishihara, and Keisuke Morishima  CELL DRIVEN NANO LOCOMOTION    2008  :230-232 
Kawai S, Yamauchi M, Wakisaka S, Ooshima T, Amano A.  Zinc-finger transcription factor odd-skipped related 2 is one of the regulators in osteoblast proliferation and bone formation.  J. Bone Miner. Res.  2007  22:1362-72  PubMed ID: 17547533
Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
Ono Y, Sensui H, Okutsu S, Nagatomi R.  Notch2 negatively regulates myofibroblastic differentiation of myoblasts.  J. Cell. Physiol.  2006  210:358-69  PubMed ID: 17044085
Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y.  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp. Hematol.  2006  34:760-9  PubMed ID: 16728281
Ouyang X, Fujimoto M, Nakagawa R, Serada S, Tanaka T, Nomura S, Kawase I, Kishimoto T, Naka T.  SOCS-2 interferes with myotube formation and potentiates osteoblast differentiation through upregulation of JunB in C2C12 cells.  J. Cell. Physiol.  2006  207:428-36  PubMed ID: 16419040
Nakanishi K, Sudo T, Morishima N.  Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development.  J. Cell Biol.  2005  169:555-60  PubMed ID: 15897261
Hata K, Nishimura R, Ueda M, Ikeda F, Matsubara T, Ichida F, Hisada K, Nokubi T, Yamaguchi A, Yoneda T.  A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.  Mol. Cell. Biol.  2005  25:1971-9  PubMed ID: 15713650
Nobta M, Tsukazaki T, Shibata Y, Xin C, Moriishi T, Sakano S, Shindo H, Yamaguchi A.  Critical regulation of bone morphogenetic protein-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.  J. Biol. Chem.  2005  280:15842-8  PubMed ID: 15695512
Morishima N, Nakanishi K, Tsuchiya K, Shibata T, Seiwa E.  Translocation of Bim to the endoplasmic reticulum (ER) mediates ER stress signaling for activation of caspase-12 during ER stress-induced apoptosis.  J. Biol. Chem.  2004  279:50375-81  PubMed ID: 15452118
Ichida F, Nishimura R, Hata K, Matsubara T, Ikeda F, Hisada K, Yatani H, Cao X, Komori T, Yamaguchi A, Yoneda T.  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J. Biol. Chem.  2004  279:34015-22  PubMed ID: 15175325
Nishimura H, Nishimura M, Oda R, Yamanaka K, Matsubara T, Ozaki Y, Sekiya K, Hamada T, Kato Y.  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp. Cell Res.  2004  295:119-27  PubMed ID: 15051495
Funato N, Ohyama K, Kuroda T, Nakamura M.  Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription.  J. Biol. Chem.  2002  278:7486-93  PubMed ID: 12493738
Morishima N, Nakanishi K, Takenouchi H, Shibata T, Yasuhiko Y.  An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12.  J. Biol. Chem.  2002  277:34287-94  PubMed ID: 12097332
Enoki Y, Watanabe H, Arake R, Sugimoto R, Imafuku T, Tominaga Y, Ishima Y, Kotani S, Nakajima M, Tanaka M, Matsushita K, Fukagawa M, Otagiri M, Maruyama T.  Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1.  Sci Rep  2016  6:32084  PubMed ID: 27549031
Kitakaze T, Sakamoto T, Kitano T, Inoue N, Sugihara F, Harada N, Yamaji R.  The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy.  Biochem. Biophys. Res. Commun.  2016  478:1292-7  PubMed ID: 27553280
Takase F, Inui A, Mifune Y, Sakata R, Muto T, Harada Y, Ueda Y, Kokubu T, Kurosaka M.  Effect of platelet-rich plasma on degeneration change of rotator cuff muscles: In vitro and in vivo evaluations.  J. Orthop. Res.  2017  35:1806-1815  PubMed ID: 27684960
Arai S, Ikeda M, Ide T, Matsuo Y, Fujino T, Hirano K, Sunagawa K, Tsutsui H.  Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation.  Am. J. Physiol., Cell Physiol.  2017  312:C29-C39  PubMed ID: 27806939
Saito N, Kimura S, Miyamoto T, Fukushima S, Amagasa M, Shimamoto Y, Nishioka C, Okamoto S, Toda C, Washio K, Asano A, Miyoshi I, Takahashi E, Kitamura H.  Macrophage ubiquitin-specific protease 2 modifies insulin sensitivity in obese mice.  Biochem Biophys Rep  2017  9:322-329  PubMed ID: 28956020
Ono A, Ito A, Sato T, Yamaguchi M, Suzuki T, Kawabe Y, Kamihira M.  Hypoxia-responsive transgene expression system using RTP801 promoter and synthetic transactivator fused with oxygen-dependent degradation domain.  J. Biosci. Bioeng.  2017  124:115-124  PubMed ID: 28284789
Go S, Go S, Veillon L, Ciampa MG, Mauri L, Sato C, Kitajima K, Prinetti A, Sonnino S, Inokuchi JI.  Altered expression of ganglioside GM3 molecular species and a potential regulatory role during myoblast differentiation.  J. Biol. Chem.  2017  292:7040-7051  PubMed ID: 28275055
Enoki Y, Watanabe H, Arake R, Fujimura R, Ishiodori K, Imafuku T, Nishida K, Sugimoto R, Nagao S, Miyamura S, Ishima Y, Tanaka M, Matsushita K, Komaba H, Fukagawa M, Otagiri M, Maruyama T.  Potential therapeutic interventions for chronic kidney disease-associated sarcopenia via indoxyl sulfate-induced mitochondrial dysfunction.  J Cachexia Sarcopenia Muscle  2017  8:735-747  PubMed ID: 28608457
Kurashina Y, Hirano M, Imashiro C, Totani K, Komotori J, Takemura K.  Enzyme-free cell detachment mediated by resonance vibration with temperature modulation.  Biotechnol. Bioeng.  2017  114:2279-2288  PubMed ID: 28627736
Yoshida K, Imamura CK, Hara K, Mochizuki M, Tanigawara Y.  Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12).  Metabolomics  2017  13:98  PubMed ID: 28781589
Ono Y, Sakamoto K.  Lipopolysaccharide inhibits myogenic differentiation of C2C12 myoblasts through the Toll-like receptor 4-nuclear factor-κB signaling pathway and myoblast-derived tumor necrosis factor-α.  PLoS ONE  2017  12:e0182040  PubMed ID: 28742154
Ogawa M, Kitano T, Kawata N, Sugihira T, Kitakaze T, Harada N, Yamaji R.  Daidzein down-regulates ubiquitin-specific protease 19 expression through estrogen receptor β and increases skeletal muscle mass in young female mice.  J. Nutr. Biochem.  2017  49:63-70  PubMed ID: 28886438
Minami K, Mori T, Nakanishi W, Shigi N, Nakanishi J, Hill JP, Komiyama M, Ariga K.  Suppression of Myogenic Differentiation of Mammalian Cells Caused by Fluidity of a Liquid-Liquid Interface.  ACS Appl Mater Interfaces  2017  9:30553-30560  PubMed ID: 28836758
Asano T, Ishizuka T, Yawo H.  Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells.  Methods Mol. Biol.  2017  1668:135-145  PubMed ID: 28842907
Yamamoto Y, Sawa R, Wake I, Morimoto A, Okimura Y.  Glucose-mediated inactivation of AMP-activated protein kinase reduces the levels of L-type amino acid transporter 1 mRNA in C2C12 cells.  Nutr Res  2017  47:13-20  PubMed ID: 29241574
Okutani C, Wagatsuma A, Mabuchi K, Hoshino T.  Directed cell migration in co-cultures by topographic curvature for heterogeneous tissue engineering.  Conf Proc IEEE Eng Med Biol Soc  2017  2017:1607-1610  PubMed ID: 29060190
Fujiwara Y, Tsukahara C, Ikeda N, Sone Y, Ishikawa T, Ichi I, Koike T, Aoki Y.  Oleuropein improves insulin resistance in skeletal muscle by promoting the translocation of GLUT4.  J Clin Biochem Nutr  2017  61:196-202  PubMed ID: 29203961
Kawai S, Yamauchi M, Amano A.  Zinc-finger transcription factor Odd-skipped related 1 regulates cranial bone formation.  J. Bone Miner. Metab.  2018    PubMed ID: 29234951
Wagatsuma A, Takayama Y, Hoshino T, Shiozuka M, Yamada S, Matsuda R, Mabuchi K.  Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway.  Mol. Cell. Biochem.  2018    PubMed ID: 29248972
Hatazawa Y, Qian K, Gong DW, Kamei Y.  PGC-1α regulates alanine metabolism in muscle cells.  PLoS ONE  2018  13:e0190904  PubMed ID: 29315328
Kumeta M, Takahashi D, Takeyasu K, Yoshimura SH.  Cell type-specific suppression of mechanosensitive genes by audible sound stimulation.  PLoS ONE  2018  13:e0188764  PubMed ID: 29385174
Kitakaze T, Oshimo M, Kobayashi Y, Ryu M, Suzuki YA, Inui H, Harada N, Yamaji R.  Lactoferrin promotes murine C2C12 myoblast proliferation and differentiation and myotube hypertrophy.  Mol Med Rep  2018  17:5912-5920  PubMed ID: 29436684
Mizuguchi Y, Yatabe M, Morishima N, Morimoto S, Ichihara A.  Buffering roles of (pro)renin receptor in starvation-induced autophagy of skeletal muscles.  Physiol Rep  2018  6:  PubMed ID: 29488348
  Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell  Sensors and Actuators B: Chemical   2016  236, 659-667 
  Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes  Journal of Food and Nutrition Research  2016  4, 498 
  Fine virtual cathode display for biomolecules control and cell nano surgery  IEEE Xplore  2017   
  On-chip internalization process of an intracellular nanobot into a single cell  IEEE Xplore  2017   
  Cell descent caused by boundary curvature of a high topographical structure for a device that changes cell density  Japanese Journal of Applied Physics  2017  06GM03 
Fujiki J, Maeda N, Sato M, Ieko T, Inoue H, Iwasaki T, Iwano H, Yokota H.  Corticosterone biosynthesis in mouse clonal myoblastic C2C12 cells.  Steroids  2018    PubMed ID: 30018003
Yamasaki M, Hamada K, Fujii K, Nishiyama K, Yamasaki Y, Tari H, Araki K, Arakawa T.  Vaccinium ashei leaves extract alleviates insulin resistance via AMPK independent pathway in C2C12 myotube model.  Biochem Biophys Rep  2018    PubMed ID: 29872751
Yuasa K, Okubo K, Yoda M, Otsu K, Ishii Y, Nakamura M, Itoh Y, Horiuchi K.  Targeted ablation of p38α MAPK suppresses denervation-induced muscle atrophy.  Sci Rep  2018    PubMed ID: 29899565
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Sakai H, Kimura M, Tsukimura Y, Yabe S, Isa Y, Kai Y, Sato F, Kon R, Ikarashi N, Narita M, Chiba Y, Kamei J.  Dexamethasone exacerbates cisplatin-induced muscle atrophy.  Clin. Exp. Pharmacol. Physiol.  2018    PubMed ID: 30137654
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Tsukamoto S, Shibasaki A, Naka A, Saito H, Iida K.  Lactate Promotes Myoblast Differentiation and Myotube Hypertrophy via a Pathway Involving MyoD In Vitro and Enhances Muscle Regeneration In Vivo.  Int J Mol Sci  2018    PubMed ID: 30463265
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Nakao M, Imashiro C, Kuribara T, Kurashina Y, Totani K, Takemura K.  Formation of Large Scaffold-Free 3-D Aggregates in a Cell Culture Dish by Ultrasound Standing Wave Trapping.  Ultrasound Med Biol  2019    PubMed ID: 30799124
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Masahito Ban, Satoshi Tobea, Louie Takeuchi  Effects of diamond-like carbon thin film and wrinkle microstructure on cell proliferation  Diamond and Related Materials  2018  90:194-201 



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