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Cell No. : Cell Name
RCB0985 : Ae  update : 2024/01/17
CommentWorm cell line established by Grace. Culturable in MGM-450-10%FBS.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Grace, T. D. C.
Year of deposit 1994
Animal _silkworm < Insect
Genus Antheraea
Species eucalypti
Tissue ovary
Classification other
History Grace, T.D.C., - Mitsuhashi, J., - Kyusyu Uni. - National Inst. Sericultural and Entomological Scien
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z109
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium Grace's Insect Medium, Supplemented + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 23-25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4919  GRACE TD.  Establishment of four strains of cells from insect tissues grown in vitro.  Nature  1962  195:788-9.  PubMed ID: 13900940   DOI: 10.1038/195788a0

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