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RCB0872 : LLC-GA5-CoL300  update : 2018/07/06
CommentPig kidney (LLC-PK1) cells expressing human P-glycoproteins.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Keio University
School of Medicine, Department of Clinical Pharmacokinetics and Pharmacodynamics
35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JAPAN
Dr.Tanigawara, Yusuke
Fax. +81-3-5269-4576



English
Address
Kyoto University
Faculty of Agriculture Division of Applied Life Sciences
Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
Dr.Ueda, Kazumitsu
Fax. +81-75-753-6104
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ueda, K. & Tanigawara, Y.
Year of deposit 1993
Original cell LLC-PK1
Animal pig < Mammals
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene human P-glycoprotein
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 + 10% FBS + 300ng/ml Colchicine
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 9


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Reference
1888  Human P-glycoprotein transports cortisol, aldosterone, and dexamethasone, but not progesterone.  Ueda K, Okamura N, Hirai M, Tanigawara Y, Saeki T, Kioka N, Komano T, Hori R  J Biol Chem  1992  267:24248-52  PubMed ID: 1360010
4897  Human P-glycoprotein transports cyclosporin A and FK506.  Saeki T1, Ueda K, Tanigawara Y, Hori R, Komano T.  J Biol Chem  1993  268(9):6077-80  PubMed ID: 7681059
4898  P-glycoprotein-mediated transcellular transport of MDR-reversing agents.  Saeki T, Ueda K, Tanigawara Y, Hori R, Komano T.  FEBS Lett  1993  324(1):99-102  PubMed ID: 8099333

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User's Publication
3432  In vitro and in vivo evaluation of the metabolism and bioavailability of ester prodrugs of mgs0039 (3-(3,4-dichlorobenzyloxy)-2-amino-6-fluorobicyclo[3.1.0]hexane-2,6-dicarb oxylic Acid), a potent metabotropic glutamate receptor antagonist.  Nakamura, Masato, Kawakita, Yasunori, Yasuhara, Akito, Fukasawa, Yoshiki, Yoshida, Koji, Sakagami, Kazunari, Nakazato, Atsuro  Drug Metab Dispos  2006  34:369-74  PubMed ID: 16326817
8458  Effects of Curcuma spp. on P-glycoprotein function.  Ampasavate C, Sotanaphun U, Phattanawasin P, Piyapolrungroj N.  Phytomedicine  2009  17:506-12  PubMed ID: 19879740
10873  Effect of acidless orange on P-glycoprotein function     Thai Journal of Pharmaceutical Sciences  2016  40:139-142 
4915  Outward transfer of dopamine precursor L-3,4-dihydroxyphenylalanine (L-dopa) by native and human P-glycoprotein in LLC-PK(1) and LLC-GA5 col300 renal cells.  Soares-Da-Silva P1, Serrão MP.  J Pharmacol Exp Ther  2000  293(2):697-704  PubMed ID: 10773046
4916  P-glycoprotein phosphorylation/dephosphorylation and cellular accumulation of L-DOPA in LLC-GA5 Col300 cells.  Sampaio-Maia B, Gomes R, Soares-da-Silva P.  J Auton Pharmacol  1999  19(3):173-9  PubMed ID: 10511474
4923  Contribution of P-glycoprotein to bunitrolol efflux across blood-brain barrier.  Matsuzaki J, Yamamoto C, Miyama T, Takanaga H, Matsuo H, Ishizuka H, Kawahara Y, Kuwano M, Naito M, Tsuruo T, Sawada Y.  Biopharm Drug Dispos  1999  20(2):85-90  PubMed ID: 10206323
4917  Contribution of P-glycoprotein to efflux of ramosetron, a 5-HT3 receptor antagonist, across the blood-brain barrier.  Yamamoto C, Murakami H, Koyabu N, Takanaga H, Matsuo H, Uchiumi T, Kuwano M, Naito M, Tsuruo T, Ohtani H, Sawada Y.  J Pharm Pharmacol  2002  54(8):1055-63  PubMed ID: 12195819
4918  Effect of furanocoumarin derivatives in grapefruit juice on the uptake of vinblastine by Caco-2 cells and on the activity of cytochrome P450 3A4.  Ohnishi A, Matsuo H, Yamada S, Takanaga H, Morimoto S, Shoyama Y, Ohtani H, Sawada Y.  Br J Pharmacol  2000  130(6):1369-77  PubMed ID: 10903978
11690  Natural Furanocoumarin as Potential Oral Absorption Enhancer  May Phyu Thein Maw, Panadda Phattanawasin, Uthai Sotanaphun, Nusara Piyapolrungroj  Key Engineering Materials  2019   



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