Cell No. : Cell Name RCB0867 : 3-2H3  update : 2024/01/17
Comment			Anti-human-myeloperoxidase MoAb producing.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Yamada, Michiyuki
		Originator	Hur, S. -J. & Yamada, M.
		Year of deposit	1993
		Animal	_mouse x mouse < Hybrid
		Tissue	hybridoma
		Classification	hybrid cells
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_J719
	hybridoma	Fusion_cell_1 name	spleen
		Fusion_cell_1 strain	mouse, BALB/c
		Fusion_cell_2 name	Sp2/0-Ag14
		Fusion_cell_2 strain	mouse
		Fusion method	PEG #4000, HAT selection
		Antibody name	3-2H3
		Subclass	IgG1
		Immunogen	Human myeloperoxidase
		Antigen_name	myeloperoxidase
		Antigen_m.w.	150000
		Antigen_character	Human origin native enzyme
		Antigen_positive	Blood granulocyte, plasma, bone marrow cells
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		DMEM (low glucose) + 10% FBS (heat inactivated)
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Antibody subclass		IgG1
Reference information		Reference	3
		User's Publication	4

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Reference

1835  Yamada M, Hur, S J, Toda H  Isolation and characterization of extracellular myeloperoxidase precursor in HL-60 cell cultures.  Biochem Biophys Res Commun  1990  166:852-9  PubMed ID: 2154223   DOI: 10.1016/0006-291x(90)90888-t

1836  Hur, S J, Toda, H K, Yamada M  Isolation and characterization of an unprocessed extracellular myeloperoxidase in HL-60 cell cultures.  J Biol Chem  1989  264:8542-8  PubMed ID: 2542279

1834  Hur, S J, Yamada M  Production and characterization of two monoclonal antibodies against human myeloperoxidase.  Biotechnol Appl Biochem  1986  8:510-5  PubMed ID: 3028450

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User's Publication

9767  Hakoda M.  Development of dielectrophoresis separator with an insulating porous membrane using DC-Offset AC Electric Fields.  Biotechnol. Prog.  2016  32:1292-1300  PubMed ID: 27453404   DOI: 10.1002/btpr.2330

8341  Hakoda M, Wakizaka Y, Hirota Y.  Separation of viable and nonviable animal cell using dielectrophoretic filter.  Biotechnol. Prog.  2010  26:1061-7  PubMed ID: 20205163   DOI: 10.1002/btpr.394

8471  Hirota Y, Hakoda M, Wakizaka Y.  Separation characteristics of animal cells using a dielectrophoretic filter.  Bioprocess Biosyst Eng  2010  33:607-12  PubMed ID: 19838737   DOI: 10.1007/s00449-009-0382-1

8929  Hakoda M, Hachisu T, Wakizaka Y, Mii S, Kitajima N.  Development of a method to analyze single cell activity by using dielectrophoretic levitation.  Biotechnol. Prog.  2005  21:1748-53  PubMed ID: 16321061   DOI: 10.1021/bp050009a